Cell Meter™ Live Cell Caspase 1 Binding Assay Kit *Green Fluorescence*

Cell Meter™ Live Cell Caspase 1 Binding Assay Kit

Green Fluorescence

Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. This Cell Meter™ Live Cell Caspase 1 Activity Assay Kit is designed to detect cell apoptosis by measuring caspase 1 activation in live cells. It is used for the quantification of activated caspase 1 activities in apoptotic cells, or for screening caspase 1 inhibitors. FAM-YVAD-FMK, the green label reagent, allows for direct detection of activated caspase 1 in apoptotic cells by fluorescence microscopy, flow cytometer, or fluorescent microplate reader. The kit provides all the essential components with an optimized assay protocol.

FAM-YVAD-FMK fluorometric detection of active caspase 1 using Kit #20108 in Jurkat cells. The cells were treated with 1 μM staurosporine for 3 hours (Red) while untreated cells were used as a control (Blue). Cells were incubated with FAM-YVAD-FMK for 1 hour at 37°C. The Fluorescence intensity (300, 000 cells/ 100 μL/well) was measured at Ex/Em = 490/525 nm (Cutoff = 515 nm) with a FlexStation microplate reader using bottom read mode.

Protocol

SDS

Catalog	Size	Price	Quantity
20108	25 Tests	Price

Citations

View all 5 citations: Citation Explorer

Cancer-derived exosomal Alu RNA promotes colorectal cancer progression

Authors:

Magliacane Trotta, Sara and Adinolfi, Antonio and D’Orsi, Luca and Panico, Sonia and Mercadante, Grazia and Mehlen, Patrick and Ambati, Jayakrishna and De Falco, Sandro and Tarallo, Valeria

Journal:

Experimental \& Molecular Medicine (2024): 1--11

Cancer-derived exosomal-Alu RNA promotes colorectal cancer progression

Authors:

Tarallo, Valeria and Trotta, Sara Magliacane and Adinolfi, Antonio and D'Orsi, Luca and Panico, Sonia and Mercadante, Grazia and Mehlen, Patrick and Ambati, Jayakrishna and De Falco, Sandro

Helicobacter pylori Secreted Protein HP1286 Triggers Apoptosis in Macrophages via TNF-Independent and ERK MAPK-Dependent Pathways

Authors:

Tavares, Raquel and Pathak, Sushil Kumar

Journal:

Frontiers in Cellular and Infection Microbiology (2017): 58

Death receptor 3 mediates necroptotic cell death

Authors:

Bittner, Sebastian and Knoll, Gertrud and Ehrenschwender, Martin

Journal:

Cellular and Molecular Life Sciences (2016): 1--12

Helicobacter pylori protein JHP0290 exhibits proliferative and anti-apoptotic effects in gastric epithelial cells

Authors:

Tavares, Raquel and Pathak, Sushil Kumar

Journal:

PloS one (2015): e0124407

References

View all 50 references: Citation Explorer

Structure of human caspase-6 in complex with Z-VAD-FMK: New peptide binding mode observed for the non-canonical caspase conformation

Authors:

Muller I, Lamers MB, Ritchie AJ, Dominguez C, Munoz-Sanjuan I, Kiselyov A.

Journal:

Bioorg Med Chem Lett (2011): 5244

Intracochlear perfusion of leupeptin and z-VAD-FMK: influence of antiapoptotic agents on gunshot-induced hearing loss

Authors:

Abaamrane L, Raffin F, Schmerber S, Sendowski I.

Journal:

Eur Arch Otorhinolaryngol (2011): 987

In vitro effect of different mediators of apoptosis on canine cranial and caudal cruciate ligament fibroblasts and its reversibility by pancaspase inhibitor zVAD.fmk

Authors:

Forterre S, Zurbriggen A, Spreng D.

Journal:

Vet Immunol Immunopathol (2011): 264

Experimental study on treatment of rabbits optic nerve injury with Caspase-3 inhibitor z-DEVD-fmk

Authors:

Zhang W, Yu JG, Wang X, Shen ZS, Zhang JK, Yan H.

Journal:

Zhonghua Yan Ke Za Zhi (2010): 1084

Caspase inhibitor ZVAD-fmk facilitates engraftment of donor hematopoietic stem cells in intra-bone marrow-bone marrow transplantation

Authors:

Imai Y, Adachi Y, Shi M, Shima C, Yanai S, Okigaki M, Yamashima T, Kaneko K, Ikehara S.

Journal:

Stem Cells Dev (2010): 461

Spectral properties

Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Flow cytometer
Excitation	FL1 channel
Emission	FL1 channel
Instrument specification(s)	FL2 channel for propidium iodide staining

Fluorescence microscope
Excitation	FITC channel
Emission	FITC Channel
Recommended plate	Black wall/clear bottom
Instrument specification(s)	TRITC channel for propidium iodide staining, DAPI channel for Hoechst staining

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on July 15, 2026

Component A: FAM-YVAD-FMK	1 vial
Component B: Washing Buffer	1 bottle (100 mL)
Component C: 500X Propidium Iodide	1 vial (100 µL)
Component D: 500X Hoechst Stain	1 vial (100 µL)