logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
Cell Meter™ Live Cell Caspase 1 Binding Assay Kit
Green Fluorescence
Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases. These inhibitors are cell permeable and non-cytotoxic. Once inside the cell, the caspase inhibitors bind covalently to the active caspases. This Cell Meter™ Live Cell Caspase 1 Activity Assay Kit is designed to detect cell apoptosis by measuring caspase 1 activation in live cells. It is used for the quantification of activated caspase 1 activities in apoptotic cells, or for screening caspase 1 inhibitors. FAM-YVAD-FMK, the green label reagent, allows for direct detection of activated caspase 1 in apoptotic cells by fluorescence microscopy, flow cytometer, or fluorescent microplate reader. The kit provides all the essential components with an optimized assay protocol.
FAM-YVAD-FMK fluorometric detection of active caspase 1 using Kit #20108 in Jurkat cells. The cells were treated with 1 μM staurosporine for 3 hours (Red) while untreated cells were used as a control (Blue). Cells were incubated with FAM-YVAD-FMK for 1 hour at 37°C. The Fluorescence intensity (300, 000 cells/ 100 μL/well) was measured at Ex/Em = 490/525 nm (Cutoff = 515 nm) with a FlexStation microplate reader using bottom read mode.
FAM-YVAD-FMK fluorometric detection of active caspase 1 using Kit #20108 in Jurkat cells. The cells were treated with 1 μM staurosporine for 3 hours (Red) while untreated cells were used as a control (Blue). Cells were incubated with FAM-YVAD-FMK for 1 hour at 37°C. The Fluorescence intensity (300, 000 cells/ 100 μL/well) was measured at Ex/Em = 490/525 nm (Cutoff = 515 nm) with a FlexStation microplate reader using bottom read mode.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
2010825 Tests
Price
 
Spectral properties
Correction factor (260 nm)0.32
Correction factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)
83000
Excitation (nm)493
Emission (nm)517
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
ExcitationFL1 channel
EmissionFL1 channel
Instrument specification(s)FL2 channel for propidium iodide staining
Fluorescence microscope
ExcitationFITC channel
EmissionFITC Channel
Recommended plateBlack wall/clear bottom
Instrument specification(s)TRITC channel for propidium iodide staining, DAPI channel for Hoechst staining
Fluorescence microplate reader
Excitation490 nm
Emission525 nm
Cutoff515 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
Contact us
Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on October 8, 2025