Name: Cell Meter™ Live Cell Caspase 3/7 and Phosphatidylserine Detection Kit *Triple Fluorescence Colors*
Brand: AAT Bioquest
SKU: 22850
Price: 348 USD
Availability: InStock

Cell Meter™ Live Cell Caspase 3/7 and Phosphatidylserine Detection Kit

Triple Fluorescence Colors

Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions. In the process of apoptosis, one of key events is the activation of caspases. The activation of caspase 3/7 is an important for the initiation of apoptosis. It has been proven that caspase 3/7 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses SR-DEVD-FMK as a fluorescent indicator to detect caspase 3/7 activities. SR-DEVD-FMK is cell permeable and nontoxic, once bound to caspases, the fluorescent reagent is retained inside the cell. The binding event prevents the caspases from further catalysis but will not stop apoptosis from proceeding. SR-DEVD-FMK is a red label reagent. Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is used to detect apoptotic cells that express phosphatidylserine (PS) on the cell surface. The appearance of PS on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis. Annexin V-dye conjugates monitor cell apoptosis through measuring the translocation of PS. The Annexin V-iFluor 488™ used in this kit is a green labeling reagent, with Ex/Em = 490/525 nm. The kit is designed to detect apoptosis by simultaneously monitoring Caspase 3/7 and Annexin V activities in mammalian cells. The kit also provides a Hoechst dye for labeling the nucleus of the whole population of the cells, and propidium iodide dye for staining necrosis cells. This kit is applicable for fluorescence microscope, flow cytometer, and fluorescence microplate reader. The kit provides all the essential components with an optimized assay protocol.

The fluorescence image analysis indicated the increased expression of caspase 3/7 (red, stained by TF3- DEVD-FMK) and Annexin V (Green, stained by Annexin V-iFluor 488™) in Jurkat cells induced by 1 μM staurosporine for 3 hour. The fluorescence images of the cells (300,000 cells/ well) were taken with Olympus fluorescence microscope through the DAPI, FITC, and TRITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown above. A: Non-induced control cells; B: Doublestaining of staurosporine-induced cells for caspase 3/7 (red) and Annexin V (green); C: Triple staining of staurosporine-induced cells for caspase 3/7(red), Annexin V (green) and nuclear (blue).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22850	100 Tests	Price

Citations

View all 7 citations: Citation Explorer

High-dose ascorbate exerts anti-tumor activities and improves inhibitory effect of carboplatin through the pro-oxidant function pathway in uterine serous carcinoma cell lines

Authors:

Shen, Xiaochang and Wang, Jiandong and Deng, Boer and Chen, Shuning and John, Catherine and Zhao, Ziyi and Sinha, Nikita and Haag, Jennifer and Sun, Wenchuan and Kong, Weimin and others,

Journal:

Gynecologic Oncology (2024): 93--102

The Effect of Therapeutic Klotho Protein on Cell Viability in HT-29 Cell Line

Authors:

Sariboyaci, Ayla Eker and Uysal, Onur and Soykan, Merve Nur and Gunes, Sibel

Enhancement of Apo2L/TRAIL signaling pathway receptors by the activation of Klotho gene with CRISPR/Cas9 in Caco-2 colon cancer cells

Authors:

Gunes, Sibel and Soykan, Merve Nur and Sariboyaci, Ayla Eker and Uysal, Onur and Sevimli, Tugba Semerci

Journal:

Medical Oncology (2021): 1--10

Helicobacter pylori secreted protein HP1286 triggers apoptosis in macrophages via TNF-independent and ERK MAPK-dependent pathways

Authors:

Tavares, Raquel and Pathak, Sushil Kumar

Journal:

Frontiers in Cellular and Infection Microbiology (2017): 58

Anthocyanin-rich blackcurrant extract inhibits proliferation of the MCF10A healthy human breast epithelial cell line through induction of G0/G1 arrest and apoptosis

Authors:

Nanashima, Naoki and Horie, Kayo and Chiba, Mitsuru and Nakano, Manabu and Maeda, Hayato and Nakamura, Toshiya

Journal:

Molecular Medicine Reports (2017): 6134--6141

References

View all 71 references: Citation Explorer

Rapid simultaneous determination of apoptosis, necrosis, and viability in sulfur mustard exposed HaCaT cell cultures

Authors:

Heinrich A, Balszuweit F, Thiermann H, Kehe K.

Journal:

Toxicol Lett (2009): 260

PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes

Authors:

Foldbjerg R, Olesen P, Hougaard M, Dang DA, Hoffmann HJ, Autrup H.

Journal:

Toxicol Lett (2009): 156

Concurrent induction of necrosis, apoptosis, and autophagy in ischemic preconditioned human livers formerly treated by chemotherapy

Authors:

Domart MC, Esposti DD, Sebagh M, Olaya N, Harper F, Pierron G, Franc B, Tanabe KK, Debuire B, Azoulay D, Brenner C, Lemoine A.

Journal:

J Hepatol (2009): 881

RAS mutations affect tumor necrosis factor-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects

Authors:

Kreeger PK, M and hana R, Alford SK, Haigis KM, Lauffenburger DA.

Journal:

Cancer Res (2009): 8191

Susceptibility of the C2 canine mastocytoma cell line to the effects of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)

Authors:

Elders RC, Baines SJ, Catchpole B.

Journal:

Vet Immunol Immunopathol (2009): 11

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm, 575/26 nm, 610/20 nm filter
Instrument specification(s)	FITC, PE, PE-Texas Red channel

Fluorescence microscope
Recommended plate	Black wall/clear bottom
Instrument specification(s)	FITC channel for Annexin V-iFluor 488™, TRITC channel for TF3-DEVD-FMK,

Fluorescence microplate reader
Excitation	490 nm, 550 nm
Emission	525 nm, 595 nm
Cutoff	515 nm, 570 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2025

Component A: TF3-DEVD-FMK	1 vial
Component B: 100X Annexin V-iFluor 488™ conjugate	1 vial (200 µL)
Component C: 500X Hoechst Stain	1 vial (100 µL)
Component D: 500X Propidium Iodide	1 vial (100 µL)
Component E: Washing Buffer	1 bottle (100 mL)