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Cell Meter™ Live Cell Caspase 3/7 and Phosphatidylserine Detection Kit
Triple Fluorescence Colors
Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions. In the process of apoptosis, one of key events is the activation of caspases. The activation of caspase 3/7 is an important for the initiation of apoptosis. It has been proven that caspase 3/7 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses SR-DEVD-FMK as a fluorescent indicator to detect caspase 3/7 activities. SR-DEVD-FMK is cell permeable and nontoxic, once bound to caspases, the fluorescent reagent is retained inside the cell. The binding event prevents the caspases from further catalysis but will not stop apoptosis from proceeding. SR-DEVD-FMK is a red label reagent. Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is used to detect apoptotic cells that express phosphatidylserine (PS) on the cell surface. The appearance of PS on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis. Annexin V-dye conjugates monitor cell apoptosis through measuring the translocation of PS. The Annexin V-iFluor 488™ used in this kit is a green labeling reagent, with Ex/Em = 490/525 nm. The kit is designed to detect apoptosis by simultaneously monitoring Caspase 3/7 and Annexin V activities in mammalian cells. The kit also provides a Hoechst dye for labeling the nucleus of the whole population of the cells, and propidium iodide dye for staining necrosis cells. This kit is applicable for fluorescence microscope, flow cytometer, and fluorescence microplate reader. The kit provides all the essential components with an optimized assay protocol.
The fluorescence image analysis indicated the increased expression of caspase 3/7 (red, stained by TF3- DEVD-FMK) and Annexin V (Green, stained by Annexin V-iFluor 488™) in Jurkat cells induced by 1 μM staurosporine for 3 hour. The fluorescence images of the cells (300,000 cells/ well) were taken with Olympus fluorescence microscope through the DAPI, FITC, and TRITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown above. A: Non-induced control cells; B: Doublestaining of staurosporine-induced cells for caspase 3/7 (red) and Annexin V (green); C: Triple staining of staurosporine-induced cells for caspase 3/7(red), Annexin V (green) and nuclear (blue).
The fluorescence image analysis indicated the increased expression of caspase 3/7 (red, stained by TF3- DEVD-FMK) and Annexin V (Green, stained by Annexin V-iFluor 488™) in Jurkat cells induced by 1 μM staurosporine for 3 hour. The fluorescence images of the cells (300,000 cells/ well) were taken with Olympus fluorescence microscope through the DAPI, FITC, and TRITC channel respectively. Individual images taken from each channel from the same cell population were merged as shown above. A: Non-induced control cells; B: Doublestaining of staurosporine-induced cells for caspase 3/7 (red) and Annexin V (green); C: Triple staining of staurosporine-induced cells for caspase 3/7(red), Annexin V (green) and nuclear (blue).
CatalogSize
Price
Quantity
22850100 Tests
Price
 
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings

Flow cytometer
Excitation488 nm laser
Emission530/30 nm, 575/26 nm, 610/20 nm filter
Instrument specification(s)FITC, PE, PE-Texas Red channel

Fluorescence microscope
Recommended plateBlack wall/clear bottom
Instrument specification(s) FITC channel for Annexin V-iFluor 488™, TRITC channel for TF3-DEVD-FMK,

Fluorescence microplate reader
Excitation490 nm, 550 nm
Emission525 nm, 595 nm
Cutoff515 nm, 570 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on October 9, 2025