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Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit
Green Fluorescence
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring Caspase 3 activation. Caspase 3 is widely accepted as a reliable indicator for cell apoptosis since the activation of caspase-3 (CPP32/apopain) is important for the initiation of apoptosis. Caspase 3 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This kit uses Z-DEVD-Rh 110-DVED-Z as a fluorogenic indicator for caspase-3 activity. Cleavage of Rh 110 peptides by caspase 3 generates strongly fluorescent Rh 110 that is monitored fluorimetrically at 520-530 nm with excitation of 480-500 nm. The kit provides all the essential components with an optimized assay protocol. The assay is robust, and can be readily adapted for high-throughput assays. Using 100 uL of reagents per well in a 96-well format, this kit provides sufficient reagents to perform 200 assays. Using 25 uL of reagents per well in a 384-well format, this kit provides sufficient reagents to perform 800 assays.
Detection of caspase 3/7 Activity in Jurkat cells. Jurkat cells were seeded on the same day at 80,000 cells/well/90 µL in a black wall/clear bottom 96-well costar plate. The cells were treated with or without 20 µM of camptothecin for 5 hours, and/or 5 µM caspase 3/7 inhibitor AC-DEVD-CHO for 10 minutes. The caspase 3/7 working solution (100 µL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 490/525 nm (Cutoff = 515 nm) using the NOVOstar instrument (BMG Labtech).
Detection of caspase 3/7 Activity in Jurkat cells. Jurkat cells were seeded on the same day at 80,000 cells/well/90 µL in a black wall/clear bottom 96-well costar plate. The cells were treated with or without 20 µM of camptothecin for 5 hours, and/or 5 µM caspase 3/7 inhibitor AC-DEVD-CHO for 10 minutes. The caspase 3/7 working solution (100 µL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 490/525 nm (Cutoff = 515 nm) using the NOVOstar instrument (BMG Labtech).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22796200 Tests
Price
 
Spectral properties
Extinction coefficient (cm -1 M -1)
80000
Excitation (nm)500
Emission (nm)522
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation490 nm
Emission525 nm
Cutoff515 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Top/Bottom read mode
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Page updated on September 25, 2025