Cell Meter™ Live Cell Caspase 3/7 Imaging Kit *Green Fluorescence*
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Extinction coefficient (cm -1 M -1) | 80000 |
Excitation (nm) | 500 |
Emission (nm) | 522 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
Extinction coefficient (cm -1 M -1) 80000 | Excitation (nm) 500 | Emission (nm) 522 |
Platform
Fluorescence microscope
Excitation | FITC filter set |
Emission | FITC filter set |
Recommended plate | Black wall/clear bottom |
Components
Example protocol
AT A GLANCE
- Prepare cells with test compounds at a density of 5 × 104 to 2 × 105 cells/100 µL/well/96-well plate
- Add equal volume of Caspase 3/7 Substrate working solution
- Incubate in a 5% CO2 incubator at 37 °C for 60 minutes
- Image with a fluorescence microscope using a FITC filter set
Thaw all the kit components at room temperature before use.
CELL PREPARATION
For guidelines on cell sample preparation, please visit https://www.aatbio.com/resources/guides/cell-sample-preparation.html
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add 50 µL of DMSO (Component C) into the vial of ApoSight™ Green Caspase 3/7 Substrate (Component A) to make 200X ApoSight™ Green Caspase 3/7 Substrate stock solution.
Note Aliquot in smaller vials to avoid repeated freeze thaw cycles. Protect from light.
PREPARATION OF WORKING SOLUTION
Prepare ApoSight™ Green Caspase 3/7 substrate working solution by mixing 5 µL of 200X ApoSight™ Green Caspase 3/7 Substrate stock solution with 1 mL of assay buffer (Component B). Mix well.
Note 100 µL of ApoSight™ Green Caspase 3/7 substrate working solution is enough for 10 tests in a 96-well plate format
Note Prepare enough ApoSight™ Green Caspase 3/7 substrate working solution right before the experiment, and use promptly.
SAMPLE EXPERIMENTAL PROTOCOL
Treat Jurkat cells with 2 μg/mL camptothecin for 3 hours
Treat Jurkat cells with 1 μM staurosporine for 3-4 hours
Treat HL-60 cells with 4 μg/mL camptothecin for 4 hours
Treat HL-60 cells with 1 μM staurosporine for 4 hours.
Note Each cell line should be evaluated on an individual basis to determine the optimal cell density for apoptosis induction.
- Prepare cells with test compounds at a density of 5 × 104 to 2 × 105 cells/100 µL/well/96-well plate.
- Add equal volume of Caspase 3/7 Substrate working solution to the cells (100 µL/well/96 well-plate).
Incubate in a 5% CO2 incubator at 37 °C for 60 minutes.
Image with a fluorescence microscope using a FITC filter set.
Spectrum

Spectral properties
Extinction coefficient (cm -1 M -1) | 80000 |
Excitation (nm) | 500 |
Emission (nm) | 522 |
Images

Citations
Authors: Tavares, Raquel and Pathak, Sushil Kumar
Journal: Frontiers in Cellular and Infection Microbiology (2017): 58
References
Authors: Sivapackiam, Jothilingam and Kabra, Shivesh and Speidel, Sylvia and Sharma, Monica and Laforest, Richard and Salter, Amber and Rettig, Michael P and Sharma, Vijay
Journal: PloS one (2019): e0215579
Authors: Hua, Anh B and Justiniano, Rebecca and Perer, Jessica and Park, Sophia L and Li, Hui and Cabello, Christopher M and Wondrak, Georg T
Journal: Cancers (2019)
Authors: Zhang, Yu and Gao, Weida and Yang, Kongbin and Tao, Haiquan and Yang, Haicheng
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2018): 1411-1421
Authors: Salcher, S and Hermann, M and Kiechl-Kohlendorfer, U and Ausserlechner, M J and Obexer, P
Journal: Molecular cancer (2017): 95
Authors: Hu, Yi and Shi, Leilei and Su, Yue and Zhang, Chuan and Jin, Xin and Zhu, Xinyuan
Journal: Biomaterials science (2017): 792-799
Authors: Ni, Cheng and Li, Cheng and Dong, Yuanlin and Guo, Xiangyang and Zhang, Yiying and Xie, Zhongcong
Journal: Molecular neurobiology (2017): 3591-3605
Authors: Heusinkveld, Harm J and van Vliet, Arie C and Nijssen, Peter C G and Westerink, Remco H S
Journal: Toxicology letters (2016): 62-9
Authors: Dokic, Ivana and Mairani, Andrea and Niklas, Martin and Zimmermann, Ferdinand and Chaudhri, Naved and Krunic, Damir and Tessonnier, Thomas and Ferrari, Alfredo and Parodi, Katia and Jäkel, Oliver and Debus, Jürgen and Haberer, Thomas and Abdollahi, Amir
Journal: Oncotarget (2016): 56676-56689
Authors: Madreiter-Sokolowski, Corina T and Gottschalk, Benjamin and Parichatikanond, Warisara and Eroglu, Emrah and Klec, Christiane and Waldeck-Weiermair, Markus and Malli, Roland and Graier, Wolfgang F
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2016): 1404-20
Authors: Flannagan, Ronald S and Heit, Bryan and Heinrichs, David E
Journal: Cellular microbiology (2016): 514-35
Application notes
FAQ
Do you offer any fluorimetric assays that measure caspase activation/activity in live cells using a flow cytometer?
Does pH and staining temperature affect Annexin V-Phosphatidylserine binding?
Does propidium iodide stain apoptotic cells?
How can I tell if my cell sample is dying?