TUNEL Assay | AAT Bioquest

Cell Meter™ Live Cell TUNEL Apoptosis Assay Kit

Red Fluorescence

DNA fragmentation represents a characteristic of late stage apoptosis. DNA fragmentation in apoptotic cells can be detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL). The TUNEL assay relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are secondarily labeled with a marker. All the existing TUNEL assays contain the highly toxic sodium cacodylate which might induces apoptosis and also decrease DNA production and DNA strands. Our Cell Meter™ TUNEL Apoptosis Assay Kit uses proprietary buffer system free of sodium cacodylate. The kit is based on the incorporation of our unique proprietary fluorescent dye into the DNA fragments that form during apoptosis. The assay is optimized for the direct detection of apoptosis in either detached or attached cells without using antibody. The kit provides all the essential components with an optimized assay protocol. It is suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer.

Fluorescence images of TUNEL reaction in HeLa cells with the treatment of 100 nM or 1 μM staurosporine (SS) for 4 hours as compare to untreated control. Cells were incubated with TUNEL working solution for 1 hour at 37ºC. The red fluorescence signal was analyzed using fluorescence microscope with a TRITC filter set. Fluorescently labeled DNA strand breaks shows intense fluorescent staining in SS treated cells.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22844	50 Tests	Price

Citations

View all 36 citations: Citation Explorer

Immuno-protective vesicle-crosslinked hydrogel for allogenic transplantation

Authors:

Wang, Yuqian and Huang, Renqi and Lu, Yougong and Liu, Mingqi and Mo, Ran

Journal:

Nature Communications (2024): 1--13

In Vitro Effects of Boric Acid on Cell Cycle, Apoptosis, and miRNAs in Medullary Thyroid Cancer Cells

Authors:

Y{\i}ld{\i}r{\i}m, Onurcan and Se{\c{c}}me, M{\"u}cahit and Dodurga, Yavuz and Mete, G{\"u}l{\c{c}}in Abban and Fenkci, Semin Melahat

Journal:

Biological Trace Element Research (2024): 1--11

Antifungal Activity of Cedrol from Cunninghamia lanceolate var. konishii against Phellinus noxius and Its Mechanism

Authors:

Hsiao, Wen-Wei and Lau, Ka-Man and Chien, Shih-Chang and Chu, Fang-Hua and Chung, Wen-Hsin and Wang, Sheng-Yang

Journal:

Plants (2024): 321

Polyploid cell dynamics and death before and after PEG-treatment of a NIH/3T3 derived culture: vinblastine effects on the regulation of cell subpopulations heterogeneity

Authors:

Spano, Alessandra and Sciola, Luigi

Journal:

Cell Division (2023): 18

Effects of boric acid on invasion, migration, proliferation, apoptosis and miRNAs in medullary thyroid cancer cells

Authors:

Y{\i}ld{\i}r{\i}m, Onurcan and Se{\c{c}}me, M{\"u}cahit and Dodurga, Yavuz and Mete, G{\"u}l{\c{c}}in Abban and Fenkci, Semin Melahat

References

View all 82 references: Citation Explorer

In situ detection of apoptosis by the TUNEL assay: an overview of techniques

Authors:

Loo DT., undefined

Journal:

Methods Mol Biol (2011): 3

Testicular apoptosis after dietary zinc deficiency: ultrastructural and TUNEL studies

Authors:

Kumari D, Nair N, Bedwal RS.

Journal:

Syst Biol Reprod Med (2011): 233

In situ localization of apoptosis using TUNEL

Authors:

Hewitson TD, Darby IA.

Journal:

Methods Mol Biol (2010): 161

Simultaneous PCNA and TUNEL labeling for testicular toxicity evaluation suggests that detection of apoptosis may be more sensitive than proliferation

Authors:

D'Andrea MR, Alicknavitch M, Nagele RG, Damiano BP.

Journal:

Biotech Histochem (2010): 195

Ultrastructure and TUNEL staining on inhibition of Rubus alceaefolius total alkaloids for apoptosis of liver in rat models of acute hepatitis

Authors:

Chen W, Hong Z, Li T, Zhao J, Lin J, Zhou J, Huang M.

Journal:

Zhongguo Zhong Yao Za Zhi (2010): 1060

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	27500
Excitation (nm)	549
Emission (nm)	648

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	660/20 nm filter
Instrument specification(s)	PE-Cy5 channel

Fluorescence microscope
Excitation	TRITC filter
Emission	TRITC filter
Recommended plate	Black wall/clear bottom

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
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Custom size	Inquire
Technical Support	Contact us
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2024

Component A: 100X Tunnelyte™ Red	1 Vial (25 µL)
Component B: Reaction Buffer	1 Bottle (10 mL)
Component C: 1000X Hoechst	1 Vial (50 µL)