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Cell Meter™ Mitochondrial Hydroxyl Radical Detection Kit
Red Fluorescence
The detection of intracellular hydroxyl radical is of central importance to understanding proper cellular redox regulation and the impact of its dysregulation on various pathologies. The hydroxyl radical ('OH) is one of the reactive oxygen species (ROS) highly reactive with other molecules to achieve stability. In general, hydroxyl radical is considered to be a harmful by-product of oxidative metabolism, which can cause molecular damage in living system. It shows an average lifetime of 10-9 nano seconds and can react with nearly every biomolecule such as nuclear DNA, mitochondrial DNA, proteins and membrane lipids. AAT Bioquest's Cell Meter™ Mitochondrial Hydroxyl Radical Detection Kit is optimized for detecting hydroxyl radical in mitochondria. MitoROS™ OH580 is live-cell permeant probe and can rapidly and selectively target hydroxyl radical in live cells. It generates red fluorescence when it reacts with 'OH, and can be easily read. Cell Meter™ Mitochondrial Hydroxyl Radical Detection Kit provides a sensitive fluorimetric probe to detect OH' in live cells with one hour incubation. This kit can be used for fluorescence microplate readers and fluorescence microscopy applications.
Fluorescence images of hydroxyl radical measurement in HeLa cells using MitoROS&trade; OH580 (Cat#16055). HeLa cells were incubated with MitoROS&trade; OH580 working solution at 37 &deg;C for 1 hour, then washed once with HHBS. Fenton Reaction: Cells were then treated with 10 &micro;M CuCl2 and 100 &micro;M H<sub>2</sub>O<sub>2</sub> in 1X HBSS buffer at 37 &deg;C for 1 hour. Control: HeLa cells were kept in 1X HBSS buffer without treatment. After washing 3 times with HHBS, HeLa cells were measured using a fluorescence microscope with a TRITC filter set (Red). Cell nuclei were stained with Hoechst 33342 (Cat#17530, Blue).
Fluorescence images of hydroxyl radical measurement in HeLa cells using MitoROS&trade; OH580 (Cat#16055). HeLa cells were incubated with MitoROS&trade; OH580 working solution at 37 &deg;C for 1 hour, then washed once with HHBS. Fenton Reaction: Cells were then treated with 10 &micro;M CuCl2 and 100 &micro;M H<sub>2</sub>O<sub>2</sub> in 1X HBSS buffer at 37 &deg;C for 1 hour. Control: HeLa cells were kept in 1X HBSS buffer without treatment. After washing 3 times with HHBS, HeLa cells were measured using a fluorescence microscope with a TRITC filter set (Red). Cell nuclei were stained with Hoechst 33342 (Cat#17530, Blue).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
16055200 Tests
Price
 
Spectral properties
Excitation (nm)576
Emission (nm)598
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationCy3/TRITC filter set
EmissionCy3/TRITC filter set
Recommended plateBlack wall/clear bottom
Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on September 25, 2025