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Cell Meter™ Mitochondrion Membrane Potential Assay Kit
Orange Fluorescence Optimized for Microplate Reader
Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions. There are a variety of parameters that can be used. This particular kit is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential (MMP). The collapse of mitochondrial membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade. This fluorimetric assay uses our proprietary cationic MitoLite™ Orange for the detection of the mitochondrial membrane potential change in cells. In normal cells, the orange fluorescence intensity is increased when MitoLite™ Orange is accumulated in the mitochondria. However, in apoptotic cells, the fluorescence intensity of MitoLite™ Orange is decreased following the collapse of MMP. Cells stained with MitoLite™ Orange can be fluorometrically monitored. Our Cell Meter™ Orange Mitochondrial Membrane Potential Assay Kit provides all the essential components with an optimized assay method. The kit can be used for screening activators and inhibitors of apoptosis. And the assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format without a wash step.
The decrease in the fluorescence intensity of MitoTell™ Orange with the addition of FCCP in HeLa cells. HeLa cells were dye loaded with MitoTell™ Orange alone or in the presence of 20 µM FCCP for 15 minutes. The fluorescence intensity of MitoTell™ Orange was measured 30 minutes after adding assay buffer with a FlexStation™ microplate reader (Molecular Devices) at Ex/Em = 540/590 nm (Cutoff = 570 nm, bottom read mode).
The decrease in the fluorescence intensity of MitoTell™ Orange with the addition of FCCP in HeLa cells. HeLa cells were dye loaded with MitoTell™ Orange alone or in the presence of 20 µM FCCP for 15 minutes. The fluorescence intensity of MitoTell™ Orange was measured 30 minutes after adding assay buffer with a FlexStation™ microplate reader (Molecular Devices) at Ex/Em = 540/590 nm (Cutoff = 570 nm, bottom read mode).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22805500 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on September 25, 2025