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Cell Meter™ Mitochondrion Membrane Potential Assay Kit
Red Fluorescence Optimized for Flow Cytometry
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used. This particular kit is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential (MMP). The collapse of MMP coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade. Our Cell Meter™ Mitochondrial Membrane Potential Assay Kit provides all the essential components with an optimized assay method. This fluorimetric assay uses our proprietary cationic MitoTell™ Red for the detection of apoptosis in cells with the loss of MMP. In normal cells, the red fluorescence intensity is increased when MitoTell™ Red is accumulated in the mitochondria. However, in apoptotic cells, the fluorescence intensity of MitoTell™ Red decreases following the collapse of MMP. Cells stained with MitoTell™ Red can be visualized with a flow cytometer at APC or Cy5 channel. The kit is optimized for screening apoptosis activators and inhibitors with a flow cytometer.
<p>The decrease in fluorescence intensity of MitoTell&trade; Red in response to CCCP treatment in Jurkat cells. Jurkat cells were loaded with MitoTell&trade; Red alone (Blue) or in the presence of 20 &mu;M (Green) or 50 &mu;M CCCP (Red) for 30 minutes. The fluorescence intensity of MitoTell&trade; Red was measured using ACEA NovoCyte flow cytometer at APC channel.</p>
<p>The decrease in fluorescence intensity of MitoTell&trade; Red in response to CCCP treatment in Jurkat cells. Jurkat cells were loaded with MitoTell&trade; Red alone (Blue) or in the presence of 20 &mu;M (Green) or 50 &mu;M CCCP (Red) for 30 minutes. The fluorescence intensity of MitoTell&trade; Red was measured using ACEA NovoCyte flow cytometer at APC channel.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22806100 tests
Price
 
Spectral properties
Excitation (nm)613
Emission (nm)631
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation640 nm laser
Emission660/20 nm filter
Instrument specification(s)APC channel
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Page updated on October 13, 2025