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Cell Meter™ Mitochondrion Membrane Potential Assay Kit
Red Fluorescence Optimized for Microplate Reader
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used. This particular kit is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential (MMP). The collapse of MMP coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade. Our Cell Meter™ Mitochondrial Membrane Potential Assay Kit provides all the essential components with an optimized assay method. This fluorimetric assay uses our proprietary cationic MitoTell™ Red for the detection of apoptosis in cells with the loss of MMP. In normal cells, the red fluorescence intensity is increased when MitoTell™ Red is accumulated in the mitochondria. However, in apoptotic cells, the fluorescence intensity of MitoTell™ Red decreases following the collapse of MMP. Cells stained with MitoTell™ Red can be either visualized with a fluorescence microscope Cy5 channel, or with a fluorescence microplate reader. The kit is optimized for screening apoptosis activators and inhibitors with a fluorescence microplate reader. And the assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format without a wash step.
Hela cells were dye-loaded with MitoTell™ Red alone or in the presence of 20 μM CCCP for 30 minutes. The fluorescence intensity of MitoTell™ Red was measured 5 minutes after adding Assay Buffer B (Component C) using (A) a fluorescence microscope with Cy5 filter set or (B) FlexStation microplate reader at Ex/Em = 610/650 nm (Cutoff = 630 nm, bottom read mode).
Hela cells were dye-loaded with MitoTell™ Red alone or in the presence of 20 μM CCCP for 30 minutes. The fluorescence intensity of MitoTell™ Red was measured 5 minutes after adding Assay Buffer B (Component C) using (A) a fluorescence microscope with Cy5 filter set or (B) FlexStation microplate reader at Ex/Em = 610/650 nm (Cutoff = 630 nm, bottom read mode).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22807500 tests
Price
 
Spectral properties
Excitation (nm)613
Emission (nm)631
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation610 nm
Emission650 nm
Cutoff630 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on September 25, 2025