Cell Meter™ Multiplexing Live, Apoptotic and Necrotic Cell Detection Kit III *Triple Fluorescence Colors*

Cell Meter™ Multiplexing Live, Apoptotic and Necrotic Cell Detection Kit III

Triple Fluorescence Colors

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used. This particular kit is designed to simultaneously monitor apoptotic, necrotic and healthy cells. Apoptosis is an active, programmed process of autonomous cellular dismantling that avoids eliciting inflammation. In apoptosis, phosphatidylserine (PS) is transferred to the outer leaflet of the plasma membrane. As a universal indicator of the initial/intermediate stages of cell apoptosis, the appearance of phosphatidylserine on the cell surface can be detected before morphological changes are observed. The PS sensor Annexin V-iFluor® 488 conjugate has green fluorescence upon binding to membrane PS. Necrosis has been characterized as passive, accidental cell death resulting from environmental perturbations with uncontrolled release of inflammatory cellular contents. Loss of plasma membrane integrity, as demonstrated by the ability of a membrane-impermeable Nuclear Blue™ DCS1 (Ex/Em = 350/461 nm) to label the nucleus, represents a straightforward approach to demonstrate late stage of apoptosis and necrosis. In addition, this kit also provides a live cell labeling dye, Cellbrite™ Red (Ex/Em = 613/631 nm), for labeling non-apoptotic healthy cells. This kit is optimized to simultaneously detect cell apoptosis (green), necrosis (blue and/or green) and healthy cells (red) with a fluorescence microscope.

Fluorescence images of HeLa cells labeled with Cell Meter™ Multiplexing Live, Apoptotic and Necrotic Detection Kit *Triple Fluorescence* (Cat#22846). HeLa cells at 100,000 cells/well/100 µL were seeded overnight in a 96-well black wall/clear bottom plate. Cells were treated with 0-500 nM staurosporine (SS) at 37<sup>o</sup>C for 4 hours (A-C), or fixed in ethanol (D), then incubated with triple fluorescence assay solution for 1 hour. The fluorescence signal was measured using a fluorescence microscope with a Cy5 filter for healthy cells (Red), FITC filter for apoptotic (Green) and DAPI filter for necrotic cells (Blue), respectively.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22846	200 Tests	Price

Citations

View all 4 citations: Citation Explorer

Transcriptional coregulator Ess2 controls survival of post-thymic CD4+ T cells through the Myc and IL-7 signaling pathways

Authors:

Takada, Ichiro and Hidano, Shinya and Takahashi, Sayuri and Yanaka, Kaori and Ogawa, Hidesato and Tsuchiya, Megumi and Yokoyama, Atsushi and Sato, Shingo and Ochi, Hiroki and Nakagawa, Tohru and others,

Journal:

Journal of Biological Chemistry (2022)

Anthocyanin-rich blackcurrant extract inhibits proliferation of the MCF10A healthy human breast epithelial cell line through induction of G0/G1 arrest and apoptosis

Authors:

Nanashima, Naoki and Horie, Kayo and Chiba, Mitsuru and Nakano, Manabu and Maeda, Hayato and Nakamura, Toshiya

Journal:

Molecular Medicine Reports (2017): 6134--6141

Clusterin signals via ApoER2/VLDLR and induces meiosis of male germ cells

Authors:

Riaz, Muhammad Assad and Stammler, Angelika and Borgers, Mareike and Konrad, Lutz

Journal:

American Journal of Translational Research (2017): 1266

Detecting Apoptosis, Autophagy, and Necrosis

Authors:

Coleman, Jack and Liu, Rui and Wang, Kathy and Kumar, Arun

Journal:

Apoptosis Methods in Toxicology (2016): 77--92

References

View all 71 references: Citation Explorer

Tumor necrosis factor inhibitors block apoptosis of human epithelial cells of the salivary glands

Authors:

Sisto M, D'Amore M, Caprio S, Mitolo V, Scagliusi P, Lisi S.

Journal:

Ann N Y Acad Sci (2009): 407

PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes

Authors:

Foldbjerg R, Olesen P, Hougaard M, Dang DA, Hoffmann HJ, Autrup H.

Journal:

Toxicol Lett (2009): 156

Concurrent induction of necrosis, apoptosis, and autophagy in ischemic preconditioned human livers formerly treated by chemotherapy

Authors:

Domart MC, Esposti DD, Sebagh M, Olaya N, Harper F, Pierron G, Franc B, Tanabe KK, Debuire B, Azoulay D, Brenner C, Lemoine A.

Journal:

J Hepatol (2009): 881

Rapid simultaneous determination of apoptosis, necrosis, and viability in sulfur mustard exposed HaCaT cell cultures

Authors:

Heinrich A, Balszuweit F, Thiermann H, Kehe K.

Journal:

Toxicol Lett (2009): 260

RAS mutations affect tumor necrosis factor-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects

Authors:

Kreeger PK, M and hana R, Alford SK, Haigis KM, Lauffenburger DA.

Journal:

Cancer Res (2009): 8191

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Fluorescence microscope
Excitation	494 nm (apoptosis) / 350 nm (necrosis) / 613 nm (live)
Emission	520 nm (apoptosis) / 461 nm (necrosis) / 631 nm (live)
Recommended plate	Black wall/clear bottom
Instrument specification(s)	FITC filter (apoptosis) / DAPI filter (necrosis) / Cy5 filter (live)

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 25, 2025

Component A: 100X Annexin V-iFluor™ 488 conjugate	1 vial (200 uL)
Component B: Assay Buffer	1 bottle (50 mL)
Component C: 200X Nuclear Blue™ DCS1	1 vial (100 uL)
Component D: 200X Cellbrite™ Red	1 vial (100 uL)