Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

Cell Meter™ No-Wash Live Cell Caspase 3/7 Activity Assay Kit *Blue Fluorescence*

The fluorescence microscope images of normal Hela cells (A) and apoptotic Hela cells (B). Hela cells were cultured in a 96-well plate, and washed twice with HHBS buffer.  ApoBrite™ U470 caspase 3/7 dye loading solution was then added to the well. After incubation for 2 h at 37 °C, the cells were washed once with HHBS buffer and treated with staurosporine (1 μM) apoptosis inducer for 1 hr. The images were acquired using a fluorescence microscope equipped with DAPI filter set.
The fluorescence microscope images of normal Hela cells (A) and apoptotic Hela cells (B). Hela cells were cultured in a 96-well plate, and washed twice with HHBS buffer.  ApoBrite™ U470 caspase 3/7 dye loading solution was then added to the well. After incubation for 2 h at 37 °C, the cells were washed once with HHBS buffer and treated with staurosporine (1 μM) apoptosis inducer for 1 hr. The images were acquired using a fluorescence microscope equipped with DAPI filter set.
Ordering information
Price ()Discontinued
Catalog Number20250
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Related products
Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence*
Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence*
Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Mitochondrial Hydroxyl Radical Detection Kit *Red Fluorescence*
Cell Meter™ Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence*
Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit *Green Fluorescence*
Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit *Optimized for Flow Cytometry*
Cell Meter™ Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *Orange Fluorescence Optimized for Microplate Reader*
Cell Meter™ Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *Red Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Live Cell Caspase 3/7 Binding Assay Kit *Green Fluorescence*
Cell Meter™ Live Cell Caspase 3/7 Binding Assay Kit *Red Fluorescence*
Cell Meter™ Live Cell Caspase 1 Binding Assay Kit *Green Fluorescence*
Cell Meter™ Live Cell Caspase 2 Binding Assay Kit *Green Fluorescence*
Cell Meter™ Live Cell Caspase 6 Binding Assay Kit *Green Fluorescence*
Cell Meter™ Live Cell Caspase 8 Binding Assay Kit *Green Fluorescence*
Cell Meter™ Live Cell Caspase 9 Binding Assay Kit *Green Fluorescence*
Cell Meter™ Live Cell Caspase 10 Binding Assay Kit *Green Fluorescence*
Cell Meter™ Live Cell Caspase 13 Binding Assay Kit *Green Fluorescence*
Cell Meter™ Fluorimetric Intracellular pH Assay Kit
Cell Meter™ Fluorimetric Cell Cytotoxicity Assay Kit
Cell Meter™ Cell Viability Assay Kit *Red Fluorescence*
Cell Meter™ Cell Viability Assay Kit *Blue Fluorescence with 405 nm Excitation*
Cell Meter™ Cell Viability Assay Kit *Blue Fluorescence*
Cell Meter™ Cell Viability Assay Kit *Green Fluorescence*
Cell Meter™ Cell Viability Assay Kit *NIR Fluorescence Optimized for Fluorescence Microplate Reader*
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Blue Fluorescence Optimized for Microplate Readers*
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Green Fluorescence Optimized for Microplate Readers*
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Red Fluorescence Optimized for Microplate Readers*
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Microplate Readers*
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Orange Fluorescence Optimized for Microplate Readers*
Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit *Blue Fluorescence*
Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit *Green Fluorescence*
Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit *Red Fluorescence*
Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Green Fluorescence*
Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Green Fluorescence*
Cell Meter™ JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Microplate Assays*
Cell Meter™ JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays*
Cell Meter™ NIR Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry*
Cell Meter™ NIR Mitochondrion Membrane Potential Assay Kit *Optimized for Microplate Reader*
Cell Meter™ Mitochondrion Membrane Potential Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Mitochondrion Membrane Potential Assay Kit *Orange Fluorescence Optimized for Microplate Reader*
Cell Meter™ Intracellular GSH Assay Kit *Optimized for Flow Cytometry*
Cell Meter™ Nuclear Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Blue Fluorescence*
Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Blue Fluorescence*
Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Red Fluorescence*
Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Red Fluorescence*
Cell Meter™ Multiplexing Caspase 3/7, 8 and 9 Activity Assay Kit *Triple Fluorescence Colors*
Cell Meter™ Generic Fluorimetric Caspase Activity Assay Kit *Green Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Generic Fluorimetric Caspase Binding Assay Kit *Red Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Red Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm*
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Excited at 405 nm*
Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Orange Fluorescence Excited at 405 nm*
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm*
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Green Fluorescence Excited at 405 nm*
Cell Meter™ APC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*
Cell Meter™ PE-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*
Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit I *Triple Fluorescence Colors*
Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Green Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Fluorimetric Fixed Cell Cycle Assay Kit *Red Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Apoptotic and Necrotic Multiplexing Detection Kit II *Triple Fluorescence Colors*
Cell Meter™ Live Cell TUNEL Apoptosis Assay Kit *Red Fluorescence*
Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Optimized for 405 nm Violet Laser Excitation*
Cell Meter™ Live Cell Caspase 3/7 and Phosphatidylserine Detection Kit *Triple Fluorescence Colors*
Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence*
Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Red Fluorescence*
Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Orange Fluorescence*
Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Deep Red Fluorescence*
Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Optimized for Flow Cytometry*
Cell Meter™ Fluorimetric Mitochondrial Superoxide Activity Assay Kit*Optimized for Flow Cytometry*
Cell Meter™ Fluorimetric Mitochondrial Superoxide Activity Assay Kit*Optimized for Microplate Reader*
Cell Meter™ Autophagy Assay Kit *Blue Fluorescence*
Cell Meter™ Autophagy Fluorescence Imaging Kit
Cell Meter™ Autophagy Assay Kit *Green Fluorescence*
Cell Meter™ FITC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*
Cell Meter™ Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *NIR Fluorescence Optimized for Microplate Reader*
Cell Meter™ Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *NIR Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Colorimetric WST-8 Cell Quantification Kit
Cell Meter™ Multiplexing Live, Apoptotic and Necrotic Cell Detection Kit III *Triple Fluorescence Colors*
Cell Meter™ 2-NBDG Glucose Uptake Assay Kit
Cell Meter™ Intracellular NADH/NADPH Fluorescence Imaging Kit *Red Fluorescence*
Cell Meter™ Intracellular NADH/NADPH Flow Cytometric Analysis Kit *Red Fluorescence*
Cell Meter™ Live Cell TUNEL Apoptosis Assay Kit *Green Fluorescence*
Cell Meter™ No Wash and Probenecid-Free Endpoint Calcium Assay Kit *Optimized for microplate reader*
Cell Meter™ Mitochondrion Membrane Potential Assay Kit *Red Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Mitochondrion Membrane Potential Assay Kit *Red Fluorescence Optimized for Microplate Reader*
Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit
Cell Meter™ Flow Cytometric Calcium Assay Kit
Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Red Fluorescence Optimized for Flow Cytometry*
Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit
Cell Meter™ Intracellular NADH/NADPH Fluorescence Imaging Kit *Deep Red Fluorescence*
Cell Meter™ Intracellular NADH/NADPH Flow Cytometric Analysis Kit *Deep Red Fluorescence*
Cell Meter™ Intracellular GSH Assay Kit *Optimized for Flow Cytometry with 405 nm excitation*
Cell Meter™ Mitochondrial Autophagy Imaging Kit *Red Fluorescence*
Cell Meter™ BX520 fixable viability dye
Cell Meter™ BX590 fixable viability dye
Cell Meter™ BX650 fixable viability dye
Cell Meter™ IX830 fixable viability dye
Cell Meter™ RX660 fixable viability dye
Cell Meter™ RX700 fixable viability dye
Cell Meter™ RX780 fixable viability dye
Cell Meter™ VX450 fixable viability dye
Cell Meter™ VX500 fixable viability dye
Cell Meter™ Live Cell Caspase 8 Binding Assay Kit *Red Fluorescence*
Cell Meter™ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit *Green Fluorescence*
Cell Meter™ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit *Red Fluorescence*
Cell Meter™ Fluorimetric Cellular Voltage Assay Kit
Cell Meter™ Cell Adhesion Assay Kit
Cell Meter™ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit *Deep Red Fluorescence*
Cell Meter™ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit *Blue Fluorescence*
Cell Meter™ Fluorescence Gap Junction Tracing Kit
Cell Meter™ Colorimetric MTT Cell Proliferation Kit
Cell Meter™ Live Cell Caspase 3/7 Imaging Kit *Green Fluorescence*
Cell Meter™ Beta-Arrestin Translocation GPCR Signaling Kit
Cell Meter™ Live Cell ATP Assay Kit
Cell Meter™ Colorimetric Cell Cytotoxicity Assay Kit
Cell Meter™ Fluorimetric Phagocytosis Assay Kit *Red Fluorescence*
Cell Meter™ Cellular Senescence Activity Assay Kit *Green Fluorescence*
Cell Meter™ Cellular Senescence Activity Assay Kit *Red Fluorescence*
Cell Meter™ Cell Proliferation Assay Kit
Show More (129)

OverviewpdfSDSpdfProtocol


The activation of caspase 3/7 is important for the initiation of apoptosis. Our Cell Meter™ No-Wash Live Cell Caspase Activity Assay Kits are based on ApoBrite™ U470, our recently developed cell-permeable fluorogenic caspase substrate, the first fluorogenic probe for the direct detection of caspase activities in live cells. ApoBrite U470 consists of three moieties including a). masked fluorophore, b). caspase-selective peptide fragment (DEVD), and c). cell-penetrating moiety. The cell-penetrating moiety carries the probe into live cells. Upon entering live cells the caspase-selective peptide fragment is cleaved by a caspase to release the masked fluorophore. The intensity of recovered fluorescence is directly related to the activity of caspase to be measured. Compared to the existing caspase assays in live cells, ApoBrite™ U470 is much more robust, convenient and accurate. It does not need a DNA interaction to be fluorescent as reported for NucView reagents. It does not inhibit caspase activity as reported for the FMK peptide probes. Although fluorescent FMK peptide inhibitors of caspases are widely used for detecting caspase activities in live cells, this technology has a few severe limitations: a). FMK caspase inhibitors have high cytotoxicity since FMK peptides bind covalently to active caspases; b). The irreversibly covalent binding of FMK peptides to caspases inhibits caspase activities, causing false positive apoptosis; c). FMK assays have extremely high background, and require intensive washings, resulting in very low through put; d). FMK peptides are not stable in aqueous solutions, and have to be used immediately.

Platform


Flow cytometer

Excitation320 nm
Emission380 nm

Fluorescence microscope

Excitation320 nm
Emission380 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)DAPI channel

Fluorescence microplate reader

Excitation320 nm
Emission380 nm
Cutoff360 nm
Recommended plateSolid black

Citations


View all 7 citations: Citation Explorer
Effects of thrombospondin-4 on pro-inflammatory phenotype differentiation and apoptosis in macrophages
Authors: Rahman, Mohammed Tanjimur and Muppala, Santoshi and Wu, Jiahui and Krukovets, Irene and Solovjev, Dmitry and Verbovetskiy, Dmitriy and Obiako, Chioma and Plow, Edward F and Stenina-Adognravi, Olga
Journal: Cell death \& disease (2020): 1--14
A fast and simple fluorometric method to detect cell death in 3D intestinal organoids
Authors: Bode, Konstantin J and Mueller, Stefanie and Schweinlin, Matthias and Metzger, Marco and Brunner, Thomas
Journal: BioTechniques (2019)
Helicobacter pylori secreted protein HP1286 triggers apoptosis in macrophages via TNF-independent and ERK MAPK-dependent pathways
Authors: Tavares, Raquel and Pathak, Sushil Kumar
Journal: Frontiers in Cellular and Infection Microbiology (2017): 58
Helicobacter pylori Secreted Protein HP1286 Triggers Apoptosis in Macrophages via TNF-Independent and ERK MAPK-Dependent Pathways
Authors: Tavares, Raquel and Pathak, Sushil Kumar
Journal: Frontiers in Cellular and Infection Microbiology (2017): 58
Death receptor 3 mediates necroptotic cell death
Authors: Bittner, Sebastian and Knoll, Gertrud and Ehrenschwender, Martin
Journal: Cellular and Molecular Life Sciences (2016): 1--12
Helicobacter pylori protein JHP0290 exhibits proliferative and anti-apoptotic effects in gastric epithelial cells
Authors: Tavares, Raquel and Pathak, Sushil Kumar
Journal: PloS one (2015): e0124407

References


View all 50 references: Citation Explorer
Structure of human caspase-6 in complex with Z-VAD-FMK: New peptide binding mode observed for the non-canonical caspase conformation
Authors: Muller I, Lamers MB, Ritchie AJ, Dominguez C, Munoz-Sanjuan I, Kiselyov A.
Journal: Bioorg Med Chem Lett (2011): 5244
Intracochlear perfusion of leupeptin and z-VAD-FMK: influence of antiapoptotic agents on gunshot-induced hearing loss
Authors: Abaamrane L, Raffin F, Schmerber S, Sendowski I.
Journal: Eur Arch Otorhinolaryngol (2011): 987
In vitro effect of different mediators of apoptosis on canine cranial and caudal cruciate ligament fibroblasts and its reversibility by pancaspase inhibitor zVAD.fmk
Authors: Forterre S, Zurbriggen A, Spreng D.
Journal: Vet Immunol Immunopathol (2011): 264
Experimental study on treatment of rabbits optic nerve injury with Caspase-3 inhibitor z-DEVD-fmk
Authors: Zhang W, Yu JG, Wang X, Shen ZS, Zhang JK, Yan H.
Journal: Zhonghua Yan Ke Za Zhi (2010): 1084
Caspase inhibitor ZVAD-fmk facilitates engraftment of donor hematopoietic stem cells in intra-bone marrow-bone marrow transplantation
Authors: Imai Y, Adachi Y, Shi M, Shima C, Yanai S, Okigaki M, Yamashima T, Kaneko K, Ikehara S.
Journal: Stem Cells Dev (2010): 461
Caspase-10-dependent cell death in Fas/CD95 signalling is not abrogated by caspase inhibitor zVAD-fmk
Authors: Lafont E, Milhas D, Teissie J, Therville N, Andrieu-Abadie N, Levade T, Benoist H, Segui B.
Journal: PLoS One (2010): e13638
Pretreatment with pancaspase inhibitor (Z-VAD-FMK) delays but does not prevent intraperitoneal heat-killed group B Streptococcus-induced preterm delivery in a pregnant mouse model
Authors: Equils O, Moffatt-Blue C, Ishikawa TO, Simmons CF, Ilievski V, Hirsch E.
Journal: Infect Dis Obstet Gynecol (2009): 749432
Attenuation of allergic contact dermatitis by Z-VAD-FMK, a broad caspase inhibitor: experiment with mice
Authors: Li YY, Yan CL, Xu W.
Journal: Zhonghua Yi Xue Za Zhi (2008): 3153
Effects of Z-FA.FMK on D-galactosamine/tumor necrosis factor-alpha-induced kidney injury and oxidative stress in mice : effects of Z-FA.FMK on TNF-alpha-mediated kidney injury
Authors: Gezginci-Oktayoglu S, Tunali S, Yanardag R, Bolkent S.
Journal: Mol Cell Biochem (2008): 9
Effect of Boc-D-Fmk on hepatocyte apoptosis after bile duct ligation in rat and survival rate after endotoxin challenge
Authors: Sheen-Chen SM, Hung KS, Eng HL.
Journal: J Gastroenterol Hepatol (2008): 1276