Cell Meter™ No-Wash Live Cell Caspase 3/7 Activity Assay Kit *Blue Fluorescence*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Related products
Overview | ![]() ![]() |
The activation of caspase 3/7 is important for the initiation of apoptosis. Our Cell Meter™ No-Wash Live Cell Caspase Activity Assay Kits are based on ApoBrite™ U470, our recently developed cell-permeable fluorogenic caspase substrate, the first fluorogenic probe for the direct detection of caspase activities in live cells. ApoBrite U470 consists of three moieties including a). masked fluorophore, b). caspase-selective peptide fragment (DEVD), and c). cell-penetrating moiety. The cell-penetrating moiety carries the probe into live cells. Upon entering live cells the caspase-selective peptide fragment is cleaved by a caspase to release the masked fluorophore. The intensity of recovered fluorescence is directly related to the activity of caspase to be measured. Compared to the existing caspase assays in live cells, ApoBrite™ U470 is much more robust, convenient and accurate. It does not need a DNA interaction to be fluorescent as reported for NucView reagents. It does not inhibit caspase activity as reported for the FMK peptide probes. Although fluorescent FMK peptide inhibitors of caspases are widely used for detecting caspase activities in live cells, this technology has a few severe limitations: a). FMK caspase inhibitors have high cytotoxicity since FMK peptides bind covalently to active caspases; b). The irreversibly covalent binding of FMK peptides to caspases inhibits caspase activities, causing false positive apoptosis; c). FMK assays have extremely high background, and require intensive washings, resulting in very low through put; d). FMK peptides are not stable in aqueous solutions, and have to be used immediately.
Platform
Flow cytometer
Excitation | 320 nm |
Emission | 380 nm |
Fluorescence microscope
Excitation | 320 nm |
Emission | 380 nm |
Recommended plate | Black wall/clear bottom |
Instrument specification(s) | DAPI channel |
Fluorescence microplate reader
Excitation | 320 nm |
Emission | 380 nm |
Cutoff | 360 nm |
Recommended plate | Solid black |
Images

Figure 1. The fluorescence microscope images of normal Hela cells (A) and apoptotic Hela cells (B). Hela cells were cultured in a 96-well plate, and washed twice with HHBS buffer. ApoBrite™ U470 caspase 3/7 dye loading solution was then added to the well. After incubation for 2 h at 37 °C, the cells were washed once with HHBS buffer and treated with staurosporine (1 μM) apoptosis inducer for 1 hr. The images were acquired using a fluorescence microscope equipped with DAPI filter set.
Citations
View all 7 citations: Citation Explorer
Effects of thrombospondin-4 on pro-inflammatory phenotype differentiation and apoptosis in macrophages
Authors: Rahman, Mohammed Tanjimur and Muppala, Santoshi and Wu, Jiahui and Krukovets, Irene and Solovjev, Dmitry and Verbovetskiy, Dmitriy and Obiako, Chioma and Plow, Edward F and Stenina-Adognravi, Olga
Journal: Cell death \& disease (2020): 1--14
Authors: Rahman, Mohammed Tanjimur and Muppala, Santoshi and Wu, Jiahui and Krukovets, Irene and Solovjev, Dmitry and Verbovetskiy, Dmitriy and Obiako, Chioma and Plow, Edward F and Stenina-Adognravi, Olga
Journal: Cell death \& disease (2020): 1--14
A fast and simple fluorometric method to detect cell death in 3D intestinal organoids
Authors: Bode, Konstantin J and Mueller, Stefanie and Schweinlin, Matthias and Metzger, Marco and Brunner, Thomas
Journal: BioTechniques (2019)
Authors: Bode, Konstantin J and Mueller, Stefanie and Schweinlin, Matthias and Metzger, Marco and Brunner, Thomas
Journal: BioTechniques (2019)
Organoids as a Test System for Cell-Stress and-Death in the Intestinal Epithelium
Authors: Bode, Konstantin
Journal: (2019)
Authors: Bode, Konstantin
Journal: (2019)
Helicobacter pylori secreted protein HP1286 triggers apoptosis in macrophages via TNF-independent and ERK MAPK-dependent pathways
Authors: Tavares, Raquel and Pathak, Sushil Kumar
Journal: Frontiers in Cellular and Infection Microbiology (2017): 58
Authors: Tavares, Raquel and Pathak, Sushil Kumar
Journal: Frontiers in Cellular and Infection Microbiology (2017): 58
Helicobacter pylori Secreted Protein HP1286 Triggers Apoptosis in Macrophages via TNF-Independent and ERK MAPK-Dependent Pathways
Authors: Tavares, Raquel and Pathak, Sushil Kumar
Journal: Frontiers in Cellular and Infection Microbiology (2017): 58
Authors: Tavares, Raquel and Pathak, Sushil Kumar
Journal: Frontiers in Cellular and Infection Microbiology (2017): 58
Death receptor 3 mediates necroptotic cell death
Authors: Bittner, Sebastian and Knoll, Gertrud and Ehrenschwender, Martin
Journal: Cellular and Molecular Life Sciences (2016): 1--12
Authors: Bittner, Sebastian and Knoll, Gertrud and Ehrenschwender, Martin
Journal: Cellular and Molecular Life Sciences (2016): 1--12
Helicobacter pylori protein JHP0290 exhibits proliferative and anti-apoptotic effects in gastric epithelial cells
Authors: Tavares, Raquel and Pathak, Sushil Kumar
Journal: PloS one (2015): e0124407
Authors: Tavares, Raquel and Pathak, Sushil Kumar
Journal: PloS one (2015): e0124407
References
View all 50 references: Citation Explorer
Structure of human caspase-6 in complex with Z-VAD-FMK: New peptide binding mode observed for the non-canonical caspase conformation
Authors: Muller I, Lamers MB, Ritchie AJ, Dominguez C, Munoz-Sanjuan I, Kiselyov A.
Journal: Bioorg Med Chem Lett (2011): 5244
Authors: Muller I, Lamers MB, Ritchie AJ, Dominguez C, Munoz-Sanjuan I, Kiselyov A.
Journal: Bioorg Med Chem Lett (2011): 5244
Intracochlear perfusion of leupeptin and z-VAD-FMK: influence of antiapoptotic agents on gunshot-induced hearing loss
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Journal: Eur Arch Otorhinolaryngol (2011): 987
Authors: Abaamrane L, Raffin F, Schmerber S, Sendowski I.
Journal: Eur Arch Otorhinolaryngol (2011): 987
In vitro effect of different mediators of apoptosis on canine cranial and caudal cruciate ligament fibroblasts and its reversibility by pancaspase inhibitor zVAD.fmk
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Journal: Vet Immunol Immunopathol (2011): 264
Authors: Forterre S, Zurbriggen A, Spreng D.
Journal: Vet Immunol Immunopathol (2011): 264
Experimental study on treatment of rabbits optic nerve injury with Caspase-3 inhibitor z-DEVD-fmk
Authors: Zhang W, Yu JG, Wang X, Shen ZS, Zhang JK, Yan H.
Journal: Zhonghua Yan Ke Za Zhi (2010): 1084
Authors: Zhang W, Yu JG, Wang X, Shen ZS, Zhang JK, Yan H.
Journal: Zhonghua Yan Ke Za Zhi (2010): 1084
Caspase inhibitor ZVAD-fmk facilitates engraftment of donor hematopoietic stem cells in intra-bone marrow-bone marrow transplantation
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Journal: Stem Cells Dev (2010): 461
Authors: Imai Y, Adachi Y, Shi M, Shima C, Yanai S, Okigaki M, Yamashima T, Kaneko K, Ikehara S.
Journal: Stem Cells Dev (2010): 461
Caspase-10-dependent cell death in Fas/CD95 signalling is not abrogated by caspase inhibitor zVAD-fmk
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Journal: PLoS One (2010): e13638
Authors: Lafont E, Milhas D, Teissie J, Therville N, Andrieu-Abadie N, Levade T, Benoist H, Segui B.
Journal: PLoS One (2010): e13638
Pretreatment with pancaspase inhibitor (Z-VAD-FMK) delays but does not prevent intraperitoneal heat-killed group B Streptococcus-induced preterm delivery in a pregnant mouse model
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Authors: Equils O, Moffatt-Blue C, Ishikawa TO, Simmons CF, Ilievski V, Hirsch E.
Journal: Infect Dis Obstet Gynecol (2009): 749432
Attenuation of allergic contact dermatitis by Z-VAD-FMK, a broad caspase inhibitor: experiment with mice
Authors: Li YY, Yan CL, Xu W.
Journal: Zhonghua Yi Xue Za Zhi (2008): 3153
Authors: Li YY, Yan CL, Xu W.
Journal: Zhonghua Yi Xue Za Zhi (2008): 3153
Effects of Z-FA.FMK on D-galactosamine/tumor necrosis factor-alpha-induced kidney injury and oxidative stress in mice : effects of Z-FA.FMK on TNF-alpha-mediated kidney injury
Authors: Gezginci-Oktayoglu S, Tunali S, Yanardag R, Bolkent S.
Journal: Mol Cell Biochem (2008): 9
Authors: Gezginci-Oktayoglu S, Tunali S, Yanardag R, Bolkent S.
Journal: Mol Cell Biochem (2008): 9
Effect of Boc-D-Fmk on hepatocyte apoptosis after bile duct ligation in rat and survival rate after endotoxin challenge
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Journal: J Gastroenterol Hepatol (2008): 1276
Authors: Sheen-Chen SM, Hung KS, Eng HL.
Journal: J Gastroenterol Hepatol (2008): 1276