Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm*

Cell Meter™ Phosphatidylserine Apoptosis Assay Kit

Blue Fluorescence Excited at 405 nm

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. Our proprietary Apopxin™ PS sensor used in this kit is small molecule-based PS sensor. The PS sensor has blue fluorescence upon binding to membrane PS. The PS stain used in the kit has the spectral properties similar to those of Pacific Blue® (Pacific Blue® is the trademark of Invitrogen). The blue fluorescent stain is well excited with the Violet Laser at 405 nm, and emits intense blue fluorescence at ~450 nm. The kit is optimized to be used with a flow cytometer equipped with a Violet Laser. It is particularly suitable for multicolor flow cytometric analysis of cells. This kit has been used for flow cytometric analysis of cells in multicolor applications in combination with fluorescent antibodies.

The detection of phosphatidylserine binding activity in Jurkat cells with Cell Meter™ Phosphatidylserine Apoptosis Assay Kit. Jurkat cells were treated without (Blue) or with 1 µM staurosporine (Red) in a 37 °C, 5% CO2 incubator for 5 hours, and then dye loaded with Apopxin™ Violet 450 for 30 minutes. The fluorescence intensity of Apopxin™ Violet 450 was measured with a FACSCalibur (Becton Dickinson) flow cytometer using violet laser.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22835	100 Tests	Price

Citations

View all 4 citations: Citation Explorer

STE20-Type Kinases MST3 and MST4 Act Non-Redundantly to Promote the Progression of Hepatocellular Carcinoma

Authors:

Caputo, Mara and Xia, Ying and Anand, Sumit Kumar and Cansby, Emmelie and Andersson, Emma and Marschall, Hanns-Ulrich and K{\"o}nigsrainer, Alfred and Peter, Andreas and Mahlapuu, Margit

The Growth, Apoptosis and Oxidative Stress in Microcystis viridis Exposed to Glyphosate

Authors:

Ye, Jing and Huang, Chen and Qiu, Zhihao and Wu, Liang and Xu, Chao

Journal:

Bulletin of Environmental Contamination and Toxicology (2019): 1--5

Integrin $\beta$3 inhibits hypoxia-induced apoptosis in cardiomyocytes

Authors:

Su, Yifan and Tian, Hua and Wei, Lijiang and Fu, Guohui and Sun, Ting

Journal:

Acta Biochimica et Biophysica Sinica (2018): 658--665

Integrin β3 inhibits hypoxia-induced apoptosis in cardiomyocytes

Authors:

Su, Yifan and Tian, Hua and Wei, Lijiang and Fu, Guohui and Sun, Ting

Journal:

Acta Biochimica et Biophysica Sinica (2018)

References

View all 135 references: Citation Explorer

Glycogen synthase kinase-3 and Omi/HtrA2 induce annexin A2 cleavage followed by cell cycle inhibition and apoptosis

Authors:

Wang CY, Lin YS, Su WC, Chen CL, Lin CF.

Journal:

Mol Biol Cell (2009): 4153

Trivalent methylated arsenical-induced phosphatidylserine exposure and apoptosis in platelets may lead to increased thrombus formation

Authors:

Bae ON, Lim KM, Noh JY, Chung SM, Kim SH, Chung JH.

Journal:

Toxicol Appl Pharmacol (2009): 144

Dynamic analysis of apoptosis using cyanine SYTO probes: from classical to microfluidic cytometry

Authors:

Wlodkowic D, Skommer J, Faley S, Darzynkiewicz Z, Cooper JM.

Journal:

Exp Cell Res (2009): 1706

Eurycomanone induce apoptosis in HepG2 cells via up-regulation of p53

Authors:

Zakaria Y, Rahmat A, Pihie AH, Abdullah NR, Houghton PJ.

Journal:

Cancer Cell Int (2009): 16

Mobilization of lysosomal calcium regulates the externalization of phosphatidylserine during apoptosis

Authors:

Mirnikjoo B, Balasubramanian K, Schroit AJ.

Journal:

J Biol Chem (2009): 6918

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	405 nm laser
Emission	450/40 nm filter
Instrument specification(s)	Pacific Blue channel

Fluorescence microscope
Excitation	Violet filter
Emission	Violet filter
Recommended plate	Black wall/clear bottom

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Fax
Email	sales@aatbio.com
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Page updated on October 25, 2025

Component A: Apopxin™ Violet 450 (100X stock solution)	1 vial (200 µL)
Component B: Assay Buffer ( 4 °C)	1 bottle (50 mL)
Component C: 100X Propidium Iodide	1 vial (100 µL)