Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Microplate Readers*

Cell Meter™ Phosphatidylserine Apoptosis Assay Kit

Deep Red Fluorescence Optimized for Microplate Readers

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This kit uses a fluorescent sensor that specifically binds PS. Our proprietary Apopxin™ PS sensor used in this kit is small molecule-based PS sensor. It has red fluorescence upon binding to membrane PS. It can be used in the formats of microplate, microscope and flow cytometer while most of other commercial apoptosis assay kits are only used with either microscope or flow cytometry platform.

Fluorescence Images of Jurkat cells in a Costar black wall/clear bottom 96-well plate stained with the Cell Meter™ Phosphatidylserine Apoptosis Assay Kit. Jurkat cells were treated without (Left) or with 20 μM camptothecin (Right) for 5 hours. The fluorescence intensity was measured using a fluorescence microscope with Cy5® channel.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22793	100 Tests	Price

Citations

View all 7 citations: Citation Explorer

STE20-Type Kinases MST3 and MST4 Act Non-Redundantly to Promote the Progression of Hepatocellular Carcinoma

Authors:

Caputo, Mara and Xia, Ying and Anand, Sumit Kumar and Cansby, Emmelie and Andersson, Emma and Marschall, Hanns-Ulrich and K{\"o}nigsrainer, Alfred and Peter, Andreas and Mahlapuu, Margit

ROR1 regulates chemo resistance in Breast Cancer via modulation of drug efflux pump ABCB1

Authors:

Norman, Fultang and Abhinav, Illendula and Jianhuang, Lin and Pandey, Manoj K and Zachary, Klase and Bela, Peethambaran

Journal:

Scientific Reports (Nature Publisher Group) (2020)

ROR1 Regulates Chemoresistance in Triple Negative Breast Cancer and Can Be Targeted with Strictinin for Selective Anti-Cancer Therapy

Authors:

Fultang, Norman

Integrin $\beta$3 inhibits hypoxia-induced apoptosis in cardiomyocytes

Authors:

Su, Yifan and Tian, Hua and Wei, Lijiang and Fu, Guohui and Sun, Ting

Journal:

Acta Biochimica et Biophysica Sinica (2018): 658--665

Tumor-selective mitochondrial network collapse induced by atmospheric gas plasma-activated medium.

Authors:

Saito, Kosuke and Asai, Tomohiko and Fujiwara, Kyoko and Sahara, Junki and Koguchi, Haruhisa and Fukuda, Noboru and Suzuki-Karasaki, Miki and Soma, Masayoshi and Suzuki-Karasaki, Yoshihiro

Journal:

Oncotarget (2016)

References

View all 92 references: Citation Explorer

Trivalent methylated arsenical-induced phosphatidylserine exposure and apoptosis in platelets may lead to increased thrombus formation

Authors:

Bae ON, Lim KM, Noh JY, Chung SM, Kim SH, Chung JH.

Journal:

Toxicol Appl Pharmacol (2009): 144

Mobilization of lysosomal calcium regulates the externalization of phosphatidylserine during apoptosis

Authors:

Mirnikjoo B, Balasubramanian K, Schroit AJ.

Journal:

J Biol Chem (2009): 6918

Evaluation of cell surface expression of phosphatidylserine in ovarian carcinoma effusions using the annexin-V/7-AAD assay: clinical relevance and comparison with other apoptosis parameters

Authors:

Dong HP, Holth A, Kleinberg L, Ruud MG, Elstr and MB, Trope CG, Davidson B, Risberg B.

Journal:

Am J Clin Pathol (2009): 756

Detection of apoptosis based on the interaction between annexin V and phosphatidylserine

Authors:

Liu T, Zhu W, Yang X, Chen L, Yang R, Hua Z, Li G.

Journal:

Anal Chem (2009): 2410

Peptidic targeting of phosphatidylserine for the MRI detection of apoptosis in atherosclerotic plaques

Authors:

Burtea C, Laurent S, Lancelot E, Ballet S, Murariu O, Rousseaux O, Port M, V and er Elst L, Corot C, Muller RN.

Journal:

Mol Pharm (2009): 1903

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Fluorescence microplate reader
Excitation	650 nm
Emission	680 nm
Cutoff	665 nm
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 25, 2025

Component A: Apopxin™ Deep Red (100X Stock Solution)	1 vial (100 µL/vial)
Component B: Assay Buffer (4 °C)	1 bottle (10 mL)