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Cell Meter™ Phosphatidylserine Apoptosis Assay Kit
Green Fluorescence Excited at 405 nm
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. Our proprietary Apopxin™ PS sensor used in this kit is small molecule-based PS sensor. The green fluorescent stain is well excited with the Violet Laser at 405 nm, and emits intense green fluorescence at ~520 nm. The kit is optimized to be used with a flow cytometer equipped with Violet Laser. It is particularly suitable for multicolor flow cytometric analysis of cells. In coupling with its large Stokes Shift, its highly enhanced affinity to PS makes this kit more robust than the other commercial Annexin V based apoptosis kits that are only used with either microscope or flow cytometry platform. This kit can be also used with a fluorescence microplate reader besides the microscope and flow cytometry platforms.
The detection of phosphatidylserine binding activity in Jurkat cells. Jurkat cells were treated without (Blue) or with 1 µM staurosporine (Red) in a 37 °C, 5% CO2 incubator for 5 hours, and then dye loaded with Apopxin™ Violet 500 for 30 minutes. The fluorescence intensity of Apopxin™ Violet 500 was measured with a FACSCalibur (Becton Dickinson) flow cytometer using violet laser.
The detection of phosphatidylserine binding activity in Jurkat cells. Jurkat cells were treated without (Blue) or with 1 µM staurosporine (Red) in a 37 °C, 5% CO2 incubator for 5 hours, and then dye loaded with Apopxin™ Violet 500 for 30 minutes. The fluorescence intensity of Apopxin™ Violet 500 was measured with a FACSCalibur (Becton Dickinson) flow cytometer using violet laser.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22836100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation405 nm laser
Emission525/40 nm filter
Instrument specification(s)AmCyan channel
Fluorescence microscope
ExcitationViolet filter
EmissionViolet filter
Recommended plateBlack wall/clear bottom
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Page updated on September 24, 2025