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Cell Meter™ Phosphatidylserine Apoptosis Assay Kit
Green Fluorescence Optimized for Microplate Readers
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This kit uses a fluorescent sensor that specifically binds PS. This kit uses our proprietary fluorescent small molecule-based Apopxin™ PS sensor that specifically binds PS with affinity much higher than Annexin V (Kd < 10 nM). It has green fluorescence upon binding to membrane PS. It can be used in the formats of microplate, microscope and flow cytometer while most of other commercial apoptosis assay kits are only used with either microscope or flow cytometry platform.
Detection of phosphatidylserine binding activity in Jurkat cells. Jurkat cells were seeded on the same day at 200,000 cells/90 &micro;L/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with different doses of camptothecin for 5 hours as indicated. The Apopxin&trade; Green assay solution (100 &micro;L/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 490/525 nm with NOVOstar instrument (from BMG Labtech) using bottom read mode.
Detection of phosphatidylserine binding activity in Jurkat cells. Jurkat cells were seeded on the same day at 200,000 cells/90 &micro;L/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with different doses of camptothecin for 5 hours as indicated. The Apopxin&trade; Green assay solution (100 &micro;L/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 490/525 nm with NOVOstar instrument (from BMG Labtech) using bottom read mode.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22791100 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation490 nm
Emission525 nm
Cutoff515 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on September 24, 2025