Cell Meter™ RX780 fixable viability dye
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1176.38 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 629 |
Emission (nm) | 767 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
See also: Cell Viability Assays, Cellular Processes, Flow Cytometry Reagents, Fluorescence Activated Cell Sorting (FACS)
Molecular weight 1176.38 | Excitation (nm) 629 | Emission (nm) 767 |
Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations. Cell Meter™ fixable viability dyes are a large family of cell-impermeable fluorescent viability dyes that are optimized to match the major excitation lasers of common flow cytometers, such as 350, 405, 488, 633 and 647 nm. These dyes are impermeant to live cells but permeant to cells with compromised membranes. They irreversibly react with amine- and thiol-containing proteins and other cellular components. Since dead or fixed cells with a compromised membrane more readily react with Cell Meter™ fixable cell stains, thus stain brighter than live cells with an intact membrane, these dyes can be used to assess live vs. dead status of mammalian cells. There are a few factors to be considered when using these dyes, e.g., the titration of each dye to ensure that live cells have minimal to no staining. Cell Meter™ RX780 fixable cell stain is optimized to be excited with the red lasers at 633/647 nm with emission at 780 nm. Compared to other commercially similar viability dyes, this fixable viability dye is much more robust and stable.
Platform
Flow cytometer
Excitation | 633/647 nm laser |
Emission | 780 nm filter |
Instrument specification(s) | APC-Cy7 channel |
Fluorescence microscope
Excitation | Cy7 filter set |
Emission | Cy7 filter set |
Recommended plate | Black wall/clear bottom |
Example protocol
AT A GLANCE
Protocol summary
- Prepare samples in HHBS (0.5 mL/assay)
- Add Cell Meter™ RX780 to the cell suspension
- Stain the cells at room temperature for 20 - 60 minutes
- Wash the cells
- Fix the cells (optional)
- Examine the sample with flow cytometer using 780 nm filter (APC-Cy7 channel) and/or fluorescence microscope using Cy7 filter set
Important
Thaw at room temperature before starting the experiment.PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
Note The unused Cell Meter™ RX780 stock solution should be divided into single use aliquots and stored at -20 °C. Avoid repeated freeze/thaw cycles.
Cell Meter™ RX780 stock solution (500X)
Add 200 µL of DMSO into the Cell Meter™ RX780 vial to make 500X stock solution.Note The unused Cell Meter™ RX780 stock solution should be divided into single use aliquots and stored at -20 °C. Avoid repeated freeze/thaw cycles.
SAMPLE EXPERIMENTAL PROTOCOL
- Treat cells as desired.
- Wash cells once with HHBS or the azide- and serum/protein-free buffer of your choice.
- Resuspend cells at 5 - 10 × 10 6/mL in HHBS or in the azide- and serum/protein-free buffer of your choice.
- Add 1 µL of 500X Cell Meter™ RX780 stock solution to 0.5 mL of cells/assay and mix it well.
- Incubate at room temperature, 5% CO2 incubator for 20 - 60 minutes, protected from light.
Note The optimal stain concentrations and incubation time should be experimentally determined for different cell lines. - Wash cells twice and resuspend cells with HHBS or the buffer of your choice.
- Fix cells as desired (optional).
- Analyze cells with a flow cytometer using 780 nm filter (APC-Cy7 channel) and/or fluorescence microscope using Cy7 filter set.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cell Meter™ RX780 fixable viability dye to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 85.007 µL | 425.033 µL | 850.065 µL | 4.25 mL | 8.501 mL |
5 mM | 17.001 µL | 85.007 µL | 170.013 µL | 850.065 µL | 1.7 mL |
10 mM | 8.501 µL | 42.503 µL | 85.007 µL | 425.033 µL | 850.065 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Product Family
Name | Excitation (nm) | Emission (nm) |
Cell Meter™ BX520 fixable viability dye | 491 | 516 |
Cell Meter™ BX590 fixable viability dye | 492 | 579 |
Cell Meter™ BX650 fixable viability dye | 518 | 654 |
Cell Meter™ IX830 fixable viability dye | 811 | 822 |
Cell Meter™ RX660 fixable viability dye | 649 | 664 |
Cell Meter™ RX700 fixable viability dye | 690 | 713 |
Cell Meter™ VX450 fixable viability dye | 406 | 445 |
Cell Meter™ VX500 fixable viability dye | 433 | 498 |
Images

Figure 1. Detection of Jurkat cell viability by Cell Meter™ fixable viability dye. Jurkat cells were treated and stained with Cell Meter™ RX780 (Cat#22536), and then fixed in 3.7% formaldehyde and analyzed by flow cytometry. The dead cell population (Blue peak) is easily distinguished from the live cell population (Red peak) with APC-Cy7 channel, and nearly identical results were obtained before and after fixation
References
View all 50 references: Citation Explorer
PGC-1α Regulates Cell Proliferation and Invasion via AKT/GSK-3β/β-catenin Pathway in Human Colorectal Cancer SW620 and SW480 Cells.
Authors: Yun, Seong-Hoon and Park, Joo-In
Journal: Anticancer research (2020): 653-664
Authors: Yun, Seong-Hoon and Park, Joo-In
Journal: Anticancer research (2020): 653-664
CFSE dilution to study human T and NK cell proliferation in vitro.
Authors: Terrén, Iñigo and Orrantia, Ane and Vitallé, Joana and Zenarruzabeitia, Olatz and Borrego, Francisco
Journal: Methods in enzymology (2020): 239-255
Authors: Terrén, Iñigo and Orrantia, Ane and Vitallé, Joana and Zenarruzabeitia, Olatz and Borrego, Francisco
Journal: Methods in enzymology (2020): 239-255
The miR-3940-5p inhibits cell proliferation of gingival mesenchymal stem cells.
Authors: Han, Xiao and Yang, Haoqing and Cao, Yangyang and Ge, Lihua and Han, Nannan and Zhang, Chen and Fan, Zhipeng and Yao, Rui
Journal: Oral diseases (2019): 1363-1373
Authors: Han, Xiao and Yang, Haoqing and Cao, Yangyang and Ge, Lihua and Han, Nannan and Zhang, Chen and Fan, Zhipeng and Yao, Rui
Journal: Oral diseases (2019): 1363-1373
Toll-Like Receptor 4 (TLR4)/Cyclooxygenase-2 (COX-2) Regulates Prostate Cancer Cell Proliferation, Migration, and Invasion by NF-κB Activation.
Authors: Wang, Wei and Wang, Jiye
Journal: Medical science monitor : international medical journal of experimental and clinical research (2018): 5588-5597
Authors: Wang, Wei and Wang, Jiye
Journal: Medical science monitor : international medical journal of experimental and clinical research (2018): 5588-5597
Evaluation of Cell Proliferation and Apoptosis in Immunotoxicity Testing.
Authors: Nagarkatti, Mitzi and Rieder, Sadiye Amcaoglu and Nagarkatti, Prakash S
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 209-230
Authors: Nagarkatti, Mitzi and Rieder, Sadiye Amcaoglu and Nagarkatti, Prakash S
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 209-230
Quantitative analysis of cell proliferation by a dye dilution assay: Application to cell lines and cocultures.
Authors: Chung, Soobin and Kim, Seol-Hee and Seo, Yuri and Kim, Sook-Kyung and Lee, Ji Youn
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2017): 704-712
Authors: Chung, Soobin and Kim, Seol-Hee and Seo, Yuri and Kim, Sook-Kyung and Lee, Ji Youn
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2017): 704-712
[Myeloid-derived suppressor cells promoted autologous B cell proliferation in rheumatoid arthritis].
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Journal: Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences (2017): 819-823
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Journal: Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences (2017): 819-823
Gag-Specific CD4 T Cell Proliferation, Plasmacytoid Dendritic Cells, and Ethnicity in Perinatally HIV-1-Infected Youths: The ANRS-EP38-IMMIP Study.
Authors: Scott-Algara, Daniel and Warszawski, Josiane and Chenadec, Jérôme Le and Didier, Céline and Montange, Thomas and Viard, Jean-Paul and Dollfus, Catherine and Avettand-Fenoel, Véronique and Rouzioux, Christine and Blanche, Stéphane and Buseyne, Florence
Journal: AIDS research and human retroviruses (2017): 21-28
Authors: Scott-Algara, Daniel and Warszawski, Josiane and Chenadec, Jérôme Le and Didier, Céline and Montange, Thomas and Viard, Jean-Paul and Dollfus, Catherine and Avettand-Fenoel, Véronique and Rouzioux, Christine and Blanche, Stéphane and Buseyne, Florence
Journal: AIDS research and human retroviruses (2017): 21-28
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Authors: Kumar, Sanjay and Vaidya, Meenal
Journal: Molecular and cellular biochemistry (2016): 29-38
Authors: Kumar, Sanjay and Vaidya, Meenal
Journal: Molecular and cellular biochemistry (2016): 29-38
Vanadium pentoxide prevents NK-92MI cell proliferation and IFNγ secretion through sustained JAK3 phosphorylation.
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Authors: Gallardo-Vera, Francisco and Diaz, Daniel and Tapia-Rodriguez, Miguel and Fortoul van der Goes, Teresa and Masso, Felipe and Rendon-Huerta, Erika and Montaño, Luis F
Journal: Journal of immunotoxicology (2016): 27-37
Application notes
FAQ
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