Cell Meter™ VX450 fixable viability dye

Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations. Cell Meter™ fixable viability dyes are a large family of cell-impermeable fluorescent viability dyes that are optimized to match the major excitation lasers of common flow cytometers, such as 350, 405, 488, 633 and 647 nm. These dyes are impermeant to live cells but permeant to cells with compromised membranes. They irreversibly react with amine- and thiol-containing proteins and other cellular components. Since dead or fixed cells with a compromised membrane more readily react with Cell Meter™ fixable cell stains, thus stain brighter than live cells with an intact membrane, these dyes can be used to assess live vs. dead status of mammalian cells. There are a few factors to be considered when using these dyes, e.g., the titration of each dye to ensure that live cells have minimal to no staining. Cell Meter™ VX450 fixable cell stain is optimized to be excited with the violet laser at 405 nm with emission at 450 nm. Compared to other commercially similar viability dyes, this fixable viability dye is much more robust and stable.

Detection of Jurkat cell viability by Cell Meter™ fixable viability dye. Jurkat cells were treated and stained with Cell Meter™ VX450 (Cat#22540), and then fixed in 3.7% formaldehyde and analyzed by flow cytometry.  The dead cell population (Blue peak)  is easily distinguished from the live cell population (Red peak)  with AmCyan channel, and nearly identical results were obtained before and after fixation.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22540	200 Tests	Price

References

View all 50 references: Citation Explorer

PGC-1α Regulates Cell Proliferation and Invasion via AKT/GSK-3β/β-catenin Pathway in Human Colorectal Cancer SW620 and SW480 Cells.

Authors:

Yun, Seong-Hoon and Park, Joo-In

Journal:

Anticancer research (2020): 653-664

CFSE dilution to study human T and NK cell proliferation in vitro.

Authors:

Terrén, Iñigo and Orrantia, Ane and Vitallé, Joana and Zenarruzabeitia, Olatz and Borrego, Francisco

Journal:

Methods in enzymology (2020): 239-255

The miR-3940-5p inhibits cell proliferation of gingival mesenchymal stem cells.

Authors:

Han, Xiao and Yang, Haoqing and Cao, Yangyang and Ge, Lihua and Han, Nannan and Zhang, Chen and Fan, Zhipeng and Yao, Rui

Journal:

Oral diseases (2019): 1363-1373

Toll-Like Receptor 4 (TLR4)/Cyclooxygenase-2 (COX-2) Regulates Prostate Cancer Cell Proliferation, Migration, and Invasion by NF-κB Activation.

Authors:

Wang, Wei and Wang, Jiye

Journal:

Medical science monitor : international medical journal of experimental and clinical research (2018): 5588-5597

Evaluation of Cell Proliferation and Apoptosis in Immunotoxicity Testing.

Authors:

Nagarkatti, Mitzi and Rieder, Sadiye Amcaoglu and Nagarkatti, Prakash S

Journal:

Methods in molecular biology (Clifton, N.J.) (2018): 209-230

Physical properties

Solvent

DMSO

Spectral properties

Excitation (nm)	406
Emission (nm)	445

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Flow cytometer
Excitation	405 nm laser
Emission	525/40 nm filter
Instrument specification(s)	AmCyan channel

Fluorescence microscope
Excitation	Violet filter set
Emission	Violet filter set
Recommended plate	Black wall/clear bottom

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Page updated on September 20, 2025