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Cell Navigator™ Exosome Fluorescence Staining Kit *Orange Fluorescence*
Product key features
The Cell Navigator™ Exosome Fluorescence Staining Kit is designed for high-efficiency labeling of EVs with minimal background interference.
- Minimized aggregation and background: Enhances solubility to reduce dye clustering and non-specific fluorescence
- Efficient EV membrane integration: Provides uniform labeling with high signal fidelity and minimal off-target staining
- Applications: Ideal for EV population analysis, cellular uptake studies, and high-resolution imaging
- Comparable alternative: Offers a comparable option to PKH26-based EV labeling kits from Sigma-Aldrich with reduced aggregation and improved detection accuracy
Product description
The Cell Navigator™ Exosome Fluorescence Staining Kit (Orange Fluorescence) enables high-efficiency labeling of extracellular vesicles (EVs) using Exsomight™ Orange, a next-generation lipophilic membrane dye designed for superior solubility and reduced aggregation. Conventional dyes such as PKH, DiO, and DiI often form aggregates, resulting in non-specific background and compromised EV detection. Exsomight™ Orange integrates seamlessly into EV membranes, minimizing signal artifacts and improving detection accuracy in fluorescence-based assays.
This kit is optimized for fluorescence nanoparticle tracking analysis (fNTA), flow cytometry, and fluorescence microscopy, providing clear differentiation between EVs and non-vesicular particles. Its stable membrane incorporation supports reproducible labeling across multiple preparations, making it ideal for EV population profiling, cellular uptake studies, and high-resolution single-vesicle imaging. By reducing background fluorescence and aggregation, the kit ensures high-fidelity results in both quantitative and qualitative EV analyses.
Example protocol
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Reconstitute Exsomight™ Orange (Component A) by adding 100 μL of DMSO (Component C) to prepare a 100X stock solution.
Note: Aliquot any unused Exsomight™ Orange stock solution as single-use aliquots and store at ≤ -20°C. Protect from light and minimize freeze-thaw cycles to maintain reagent stability.
SAMPLE EXPERIMENTAL PROTOCOL
This protocol serves as a general guideline and should be adapted to meet the specific requirements of your experiment.
Extract exosomes following your standard protocol.
Note: Optimal cell density should be determined individually for each cell line.
Note: For exosome isolation from cell culture medium, the ReadiPrep™ Exosome Isolation Kit (AAT Cat# 60204) is recommended.
Add 100 µL of the exosome solution into a centrifuge tube.
Add 1 µL of Exsomight™ Orange stock solution (100X) and incubate at room temperature for 30–60 minutes.
Note: The optimal incubation time varies based on cell type and exosome concentration. Adjust incubation duration accordingly for each experiment to achieve optimal labeling.
Add 1 µL of Exsomight™ Quench Buffer (Component B) to the vial to neutralize any unreacted Exsomight™ Orange dye.
Centrifuge the labeled exosomes at 13,000 RPM for 1 hour to pellet them, then carefully resuspend the pellet in 100 µL of PBS.
Labeled exosomes can be utilized to investigate endocytosis and other downstream cellular processes.
Safety data sheet