The Cell Navigator® Exosome Fluorescence Staining Kit (Orange Fluorescence) enables high-efficiency labeling of extracellular vesicles (EVs) using Exsomight™ Orange, a next-generation lipophilic membrane dye designed for superior solubility and reduced aggregation. Conventional dyes such as PKH, DiO, and DiI often form aggregates, resulting in non-specific background and compromised EV detection. Exsomight™ Orange integrates seamlessly into EV membranes, minimizing signal artifacts and improving detection accuracy in fluorescence-based assays. This kit is optimized for fluorescence nanoparticle tracking analysis (fNTA), flow cytometry, and fluorescence microscopy, providing clear differentiation between EVs and non-vesicular particles. Its stable membrane incorporation supports reproducible labeling across multiple preparations, making it ideal for EV population profiling, cellular uptake studies, and high-resolution single-vesicle imaging. By reducing background fluorescence and aggregation, the kit ensures high-fidelity results in both quantitative and qualitative EV analyses.