Cell Navigator® F-Actin Labeling Kit *Green Fluorescence*
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12352200 |
Alternative formats
Cell Navigator® F-Actin Labeling Kit *Blue Fluorescence* |
Cell Navigator® F-Actin Labeling Kit *Orange Fluorescence* |
Cell Navigator® F-Actin Labeling Kit *Red Fluorescence* |
Related products
Overview | ![]() ![]() |
See also: Cell Structures and Organelles, Mitochondria
Correction Factor (260 nm) 0.21 | Correction Factor (280 nm) 0.11 | Extinction coefficient (cm -1 M -1) 750001 | Excitation (nm) 491 | Emission (nm) 516 | Quantum yield 0.91 |
Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to label F-actins of fixed cells in green fluorescence. The kit uses a green fluorescent phalloidin conjugate that is selectively bound to F-actins. This green fluorescent phalloidin conjugate is a high-affinity probe for F-actins with much higher photostability than the fluorescein-phalloidin conjugates. Used at nanomolar concentrations, phallotoxins are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. The labeling protocol is robust, requiring minimal hands-on time. The kit provides all the essential components with an optimized staining protocol.
Platform
Fluorescence microscope
Excitation | FITC filter |
Emission | FITC filter |
Recommended plate | Black wall/clear bottom |
Components
Example protocol
AT A GLANCE
Protocol summary
- Prepare samples (microplate wells)
- Remove the liquid from the plate
- Add 100 µL/well of iFluor™ 488-Phalloidin working solution
- Stain the cells at RT for 15 to 60 minutes
- Wash the cells
- Examine the specimen under fluorescence microscope at Ex/Em = 490/520 nm (FITC filter set)
Important notes
Thaw all the components at room temperature before starting the experiment.
PREPARATION OF WORKING SOLUTION
Add 10 μL of iFluor™ 488-Phalloidin (Component A) to 10 mL of Labeling Buffer (Component B) to make 1X iFluor™ 488-Phalloidin working solution. Protect from light. Note: Different cell types might be stained differently. The concentration of iFluor™ 488-Phalloidin working solution should be prepared accordingly.
For guidelines on cell sample preparation, please visit
https://www.aatbio.com/resources/guides/cell-sample-preparation.html
SAMPLE EXPERIMENTAL PROTOCOL
- Perform formaldehyde fixation. Incubate the cells with 3.0% – 4.0% formaldehyde in PBS at room temperature for 10 – 30 minutes. Note: Avoid any methanol containing fixatives since methanol can disrupt actin during the fixation process. The preferred fixative is methanol-free formaldehyde.
- Rinse the fixed cells 2 – 3 times in PBS.
- Optional: Add 0.1% Triton X-100 in PBS into fixed cells for 3 to 5 minutes to increase permeability. Rinse the cells 2 – 3 times in PBS.
- Add 100 µL/well (96-well plate) of iFluor™ 488-Phalloidin working solution into the fixed cells.
- Stain the cells at room temperature for 15 to 60 minutes.
- Rinse cells gently with PBS 2 to 3 times to remove excess dye before plate sealing.
- Image cells using a fluorescence microscope with FITC filter set (Ex/Em = 490/520 nm).
Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
Cell Navigator® F-Actin Labeling Kit *Blue Fluorescence* | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
Cell Navigator® F-Actin Labeling Kit *Orange Fluorescence* | 541 | 557 | 1000001 | 0.671 | 0.25 | 0.15 |
Cell Navigator® F-Actin Labeling Kit *Red Fluorescence* | 588 | 604 | 1800001 | 0.531 | 0.05 | 0.04 |
Images

Figure 1. Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with Cell Navigator® F-Actin Labeling Kit *Green Fluorescence* in a Costar black 96-well plate. Cell were labeled with iFluor® 488-Phalloidin (Cat#22261, Green) and nuclei stain DAPI (Cat#17507, Blue), respectively. Cell endoplasmic reticulum (ER) was stained with ER Red™ (Cat#22636, Red) before fixation.
Citations
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Enhancing osteoinduction and bone regeneration of biphasic calcium phosphate scaffold thought modulating the balance between pro-osteogenesis and anti-osteoclastogenesis by zinc doping
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Journal: Materials Today Chemistry (2023): 101410
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Adjusting physicochemical and cytological properties of biphasic calcium phosphate by magnesium substitution: An in vitro study
Authors: Lu, Teliang and Miao, Yali and Yuan, Xinyuan and Zhang, Yu and Ye, Jiandong
Journal: Ceramics International (2023)
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CircRPAP2 regulates the alternative splicing of PTK2 by binding to SRSF1 in breast cancer
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Authors: Yu, Yunhe and Fang, Lin
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Journal: Molecular Biology Reports (2022): 9217--9229
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Journal: Molecular Biology Reports (2022): 9217--9229
A targetable pathway in neutrophils mitigates both arterial and venous thrombosis
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Zinc-doped calcium silicate additive accelerates early angiogenesis and bone regeneration of calcium phosphate cement by double bioactive ions stimulation and immunoregulation
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Journal: Biomaterials Advances (2022): 213120
Authors: Lu, Teliang and Wang, Jinchao and Yuan, Xinyuan and Tang, Chenyu and Wang, Xiaolan and He, Fupo and Ye, Jiandong
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Authors: Shan, Zhongshu and Zhao, Yanyan and Qiu, Zhixue and Angxiu, Suonan and Gu, Yong and Luo, Junming and Bi, Hongtao and Luo, Wei and Xiong, Rui and Ma, Siqing and others,
Journal: Annals of Translational Medicine (2021)
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CHPF Regulates the Aggressive Phenotypes of Hepatocellular Carcinoma Cells via the Modulation of the Decorin and TGF-$\beta$ Pathways
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