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Cell Navigator® F-Actin Labeling Kit
Green Fluorescence
Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to label F-actins of fixed cells in green fluorescence. The kit uses a green fluorescent phalloidin conjugate that is selectively bound to F-actins. This green fluorescent phalloidin conjugate is a high-affinity probe for F-actins with much higher photostability than the fluorescein-phalloidin conjugates. Used at nanomolar concentrations, phallotoxins are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. The labeling protocol is robust, requiring minimal hands-on time. The kit provides all the essential components with an optimized staining protocol.
<p>Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with Cell Navigator® F-Actin Labeling Kit *Green Fluorescence* in a Costar black 96-well plate. Cell were labeled with iFluor® 488-Phalloidin (Cat#22261, Green) and nuclei stain DAPI (Cat#17507, Blue), respectively. Cell endoplasmic reticulum (ER) was stained with ER Red&trade; (Cat#22636, Red) before fixation.</p>
<p>Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with Cell Navigator® F-Actin Labeling Kit *Green Fluorescence* in a Costar black 96-well plate. Cell were labeled with iFluor® 488-Phalloidin (Cat#22261, Green) and nuclei stain DAPI (Cat#17507, Blue), respectively. Cell endoplasmic reticulum (ER) was stained with ER Red&trade; (Cat#22636, Red) before fixation.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22661500 Tests
Price
 
Spectral properties
Correction factor (260 nm)0.21
Correction factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)
75000
1
Excitation (nm)491
Emission (nm)516
Quantum yield
0.9
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationFITC filter
EmissionFITC filter
Recommended plateBlack wall/clear bottom
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Page updated on September 25, 2025