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Cell Navigator® F-Actin Labeling Kit
Orange Fluorescence
Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to label F-actins of fixed cells in red fluorescence. The kit uses a n orange fluorescent phalloidin conjugate that is selectively bound to F-actins. This red fluorescent phalloidin conjugate is a high-affinity probe for F-actins. Used at nanomolar concentrations, phallotoxins are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. The labeling protocol is robust, requiring minimal hands-on time. The kit provides all the essential components with an optimized staining protocol.
Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with Cell Navigator® F-Actin Labeling Kit *Orange Fluorescence* in a Costar black 96-well plate. Cells were labeled with iFluor® 546-Phalloidin (Cat#22663, Red) and nuclei stain DAPI (Cat#17507, Blue), respectively.  
Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with Cell Navigator® F-Actin Labeling Kit *Orange Fluorescence* in a Costar black 96-well plate. Cells were labeled with iFluor® 546-Phalloidin (Cat#22663, Red) and nuclei stain DAPI (Cat#17507, Blue), respectively.  
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22663500 Tests
Price
 
Spectral properties
Correction factor (260 nm)0.25
Correction factor (280 nm)0.15
Extinction coefficient (cm -1 M -1)
100000
1
Excitation (nm)541
Emission (nm)557
Quantum yield
0.67
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationTRITC filter
EmissionTRITC filter
Recommended plateBlack wall/clear bottom
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Page updated on September 24, 2025