Cell Navigator® F-Actin Labeling Kit *Orange Fluorescence*

Cell Navigator® F-Actin Labeling Kit

Orange Fluorescence

Cell Navigator® F-Actin Labeling Kit is designed for selective orange fluorescent labeling of filamentous actin (F-actin) in fixed, permeabilized cells.

Selective F-Actin Labeling: Uses iFluor® 546-Phalloidin to bind filamentous actin
Orange Fluorescence Detection: Provides excitation and emission maxima of approximately 541 nm and 557 nm, respectively
Convenient Kit Format: Includes fluorescent phalloidin and labeling buffer for 500 tests
Applications: Supports fluorescence imaging of actin filaments and cytoskeletal organization in fixed samples

Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with Cell Navigator® F-Actin Labeling Kit *Orange Fluorescence* in a Costar black 96-well plate. Cells were labeled with iFluor® 546-Phalloidin (Cat#22663, Red) and nuclei stain DAPI (Cat#17507, Blue), respectively.

Protocol

SDS

Catalog	Size	Price	Quantity
22663	500 Tests	Price

Overview

Cell Navigator® F-Actin Labeling Kit uses iFluor® 546-Phalloidin to selectively label F-actin with orange fluorescence. The fluorescent phalloidin conjugate binds actin filaments, enabling visualization of the actin cytoskeleton in formaldehyde-fixed, permeabilized cells and tissue sections.

The kit includes iFluor® 546-Phalloidin and labeling buffer, with sufficient reagents for 500 tests. The probe has excitation and emission maxima of approximately 541 nm and 557 nm, respectively, and can be imaged using a TRITC-compatible filter set. Cell Navigator® F-Actin Labeling Kit supports fluorescence microscopy studies of actin filament distribution and cytoskeletal organization.

Citations

View all 29 citations: Citation Explorer

Orchestrated Adhesive Anisotropic/Anti-Adhesive Isotropic Janus Conductive Patch Reverses Ventricular Remodeling via Synchronized Electrophysiological Reconstitution and Mitochondrial-Targeted Salvage

Authors:

Chen, Shuhui and Zhang, Xin and Luo, Guangbing and Zhao, Qiuyuan and shen, Jie and Zhan, Jiamian and Xie, Qing and Hua, Shaofeng and Hou, Honghao and He, Yutong and others,

Journal:

Advanced Functional Materials (2025): e19577

Engineered Extracellular Vesicles Derived from Juvenile Mice Enhance Mitochondrial Function in the Aging Bone Microenvironment and Achieve Rejuvenation

Authors:

Zheng, Jiaqian and Ren, Yipeng and Ke, Junhua and Zhu, Guanglin and Wang, Zhen and Shi, Xuetao and Wang, Yingjun

Journal:

ACS nano (2025)

Biphasic Calcium Phosphate Ceramic Scaffold Composed of Zinc Doped $\beta$-Tricalcium Phosphate and Silicon Doped Hydroxyapatite for Bone Tissue Engineering

Authors:

Fan, Jiajia and Yuan, Xinyuan and Lu, Teliang and Ye, Jiandong

Journal:

ACS Applied Bio Materials (2024)

Evolution of phase, morphology, physicochemical properties, and biological properties of HA ceramic with the increase of crystallinity

Authors:

Zhang, Luhui and Liang, Xinzhi and Chen, Ji and Kang, Zhengyang and Ye, Jiandong and Xie, Denghui

Journal:

Ceramics International (2024)

Temperature-Controlled Screening of Catechol Groups in Poly (N-Isopropylacrylamide-co-Dopamine Methacrylamide) for Cell Detachment

Authors:

Yang, Liuxin and Ren, Pengfei and Wei, Dandan and Liang, Min and Xu, Li and Tao, Yinghua and Jiao, Guanhua and Zhang, Tianzhu and Zhang, Qianli

Journal:

ACS Applied Polymer Materials (2024)

References

View all 42 references: Citation Explorer

Velocity distributions of single F-actin trajectories from a fluorescence image series using trajectory reconstruction and optical flow mapping

Authors:

von Wegner F, Ober T, Weber C, Schurmann S, Winter R, Friedrich O, Fink RH, Vogel M.

Journal:

J Biomed Opt (2008): 54018

Visualization of F-actin and G-actin equilibrium using fluorescence resonance energy transfer (FRET) in cultured cells and neurons in slices

Authors:

Okamoto K, Hayashi Y.

Journal:

Nat Protoc (2006): 911

The effect of F-actin on the relay helix position of myosin II, as revealed by tryptophan fluorescence, and its implications for mechanochemical coupling

Authors:

Conibear PB, Malnasi-Csizmadia A, Bagshaw CR.

Journal:

Biochemistry (2004): 15404

Analysis of models of F-actin using fluorescence resonance energy transfer spectroscopy

Authors:

Moens PD, dos Remedios CG.

Journal:

Results Probl Cell Differ (2001): 59

Fluorescence studies of the carboxyl-terminal domain of smooth muscle calponin effects of F-actin and salts

Authors:

Bartegi A, Roustan C, Kassab R, Fattoum A.

Journal:

Eur J Biochem (1999): 335

Spectral properties

Correction factor (260 nm)	0.25
Correction factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	541
Emission (nm)	557
Quantum yield	0.67 ¹

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microscope
Excitation	TRITC filter
Emission	TRITC filter
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 28, 2026

Component A: iFluor® 546-Phalloidin	1 vial (50 µL)
Component B: Labeling Buffer	1 bottle (50 mL)