Cell Navigator® F-Actin Labeling Kit *Orange Fluorescence*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Correction Factor (260 nm) | 0.25 |
Correction Factor (280 nm) | 0.15 |
Extinction coefficient (cm -1 M -1) | 1000001 |
Excitation (nm) | 541 |
Emission (nm) | 557 |
Quantum yield | 0.671 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12352200 |
Alternative formats
Cell Navigator® F-Actin Labeling Kit *Blue Fluorescence* |
Cell Navigator® F-Actin Labeling Kit *Green Fluorescence* |
Cell Navigator® F-Actin Labeling Kit *Red Fluorescence* |
Related products
Overview | ![]() ![]() |
See also: Mitochondria, Cytoskeleton Structure & Analysis
Correction Factor (260 nm) 0.25 | Correction Factor (280 nm) 0.15 | Extinction coefficient (cm -1 M -1) 1000001 | Excitation (nm) 541 | Emission (nm) 557 | Quantum yield 0.671 |
Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to label F-actins of fixed cells in red fluorescence. The kit uses a n orange fluorescent phalloidin conjugate that is selectively bound to F-actins. This red fluorescent phalloidin conjugate is a high-affinity probe for F-actins. Used at nanomolar concentrations, phallotoxins are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. The labeling protocol is robust, requiring minimal hands-on time. The kit provides all the essential components with an optimized staining protocol.
Platform
Fluorescence microscope
Excitation | TRITC filter |
Emission | TRITC filter |
Recommended plate | Black wall/clear bottom |
Components
Example protocol
AT A GLANCE
Protocol summary
- Prepare samples (microplate wells)
- Remove the liquid from the plate
- Add 100 µL/well of iFluor™ 546-Phalloidin working solution
- Incubate the cells at room temperature for 15 to 60 minutes
- Wash the cells
- Examine the specimen under fluorescence microscope at Ex/Em = 550/575 nm (TRITC filter set)
Important notes
Thaw all the components at room temperature before starting the experiment.
PREPARATION OF WORKING SOLUTION
Add 10 μL of iFluor™ 546-Phalloidin (Component A) to 10 mL of Labeling Buffer (Component B) to make 1X iFluor™ 546-Phalloidin working solution. Protect from light. Note: Different cell types might be stained differently. The concentration of iFluor™ 546-Phalloidin working solution should be prepared accordingly.
For guidelines on cell sample preparation, please visit
https://www.aatbio.com/resources/guides/cell-sample-preparation.html
SAMPLE EXPERIMENTAL PROTOCOL
- Perform formaldehyde fixation. Incubate the cells with 3.0% – 4.0% formaldehyde in PBS at room temperature for 10 – 30 minutes. Note: Avoid any methanol containing fixatives since methanol can disrupt actin during the fixation process. The preferred fixative is methanol-free formaldehyde.
- Rinse the fixed cells 2 – 3 times in PBS.
- Optional: Add 0.1% Triton X-100 in PBS into fixed cells for 3 to 5 minutes to increase permeability. Rinse the cells 2 – 3 times in PBS.
- Add 100 µL/well (96-well plate) of iFluor™ 546-Phalloidin working solution into the fixed cells.
- Incubate the cells at room temperature for 15 to 60 minutes.
- Rinse cells gently with PBS 2 to 3 times to remove excess dye before plate sealing.
- Image cells using a fluorescence microscope with TRITC filter set (Ex/Em = 550/575 nm).
Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Correction Factor (260 nm) | 0.25 |
Correction Factor (280 nm) | 0.15 |
Extinction coefficient (cm -1 M -1) | 1000001 |
Excitation (nm) | 541 |
Emission (nm) | 557 |
Quantum yield | 0.671 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
Cell Navigator® F-Actin Labeling Kit *Blue Fluorescence* | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
Cell Navigator® F-Actin Labeling Kit *Green Fluorescence* | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
Cell Navigator® F-Actin Labeling Kit *Red Fluorescence* | 588 | 604 | 1800001 | 0.531 | 0.05 | 0.04 |
Images
Citations
View all 23 citations: Citation Explorer
Modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide CREKA for enhanced bone repair
Authors: Wu, Qi and Fu, Xiaoling and Li, Xian and Li, Jing and Han, Weiju and Wang, Yingjun
Journal: Bioactive Materials (2023): 208--220
Authors: Wu, Qi and Fu, Xiaoling and Li, Xian and Li, Jing and Han, Weiju and Wang, Yingjun
Journal: Bioactive Materials (2023): 208--220
Enhancing osteoinduction and bone regeneration of biphasic calcium phosphate scaffold thought modulating the balance between pro-osteogenesis and anti-osteoclastogenesis by zinc doping
Authors: Lu, T and Yuan, X and Zhang, L and He, F and Wang, X and Ye, J
Journal: Materials Today Chemistry (2023): 101410
Authors: Lu, T and Yuan, X and Zhang, L and He, F and Wang, X and Ye, J
Journal: Materials Today Chemistry (2023): 101410
Microfluidic Chip-Based Modeling of Three-Dimensional Intestine--Vessel--Liver Interactions in Fluorotelomer Alcohol Biotransformation
Authors: Xu, Ning and Lin, Haifeng and Lin, Jin-Ming and Cheng, Jie and Wang, Peilong and Lin, Ling
Journal: Analytical Chemistry (2023)
Authors: Xu, Ning and Lin, Haifeng and Lin, Jin-Ming and Cheng, Jie and Wang, Peilong and Lin, Ling
Journal: Analytical Chemistry (2023)
Adjusting physicochemical and cytological properties of biphasic calcium phosphate by magnesium substitution: An in vitro study
Authors: Lu, Teliang and Miao, Yali and Yuan, Xinyuan and Zhang, Yu and Ye, Jiandong
Journal: Ceramics International (2023)
Authors: Lu, Teliang and Miao, Yali and Yuan, Xinyuan and Zhang, Yu and Ye, Jiandong
Journal: Ceramics International (2023)
CircRPAP2 regulates the alternative splicing of PTK2 by binding to SRSF1 in breast cancer
Authors: Yu, Yunhe and Fang, Lin
Journal: Cell death discovery (2022): 1--12
Authors: Yu, Yunhe and Fang, Lin
Journal: Cell death discovery (2022): 1--12
A targetable pathway in neutrophils mitigates both arterial and venous thrombosis
Authors: Nayak, Lalitha and Sweet, David R and Thomas, Asha and Lapping, Stephanie D and Kalikasingh, Kenneth and Madera, Annmarie and Vinayachandran, Vinesh and Padmanabhan, Roshan and Vasudevan, Neelakantan T and Myers, Jay T and others,
Journal: Science Translational Medicine (2022): eabj7465
Authors: Nayak, Lalitha and Sweet, David R and Thomas, Asha and Lapping, Stephanie D and Kalikasingh, Kenneth and Madera, Annmarie and Vinayachandran, Vinesh and Padmanabhan, Roshan and Vasudevan, Neelakantan T and Myers, Jay T and others,
Journal: Science Translational Medicine (2022): eabj7465
Zinc-doped calcium silicate additive accelerates early angiogenesis and bone regeneration of calcium phosphate cement by double bioactive ions stimulation and immunoregulation
Authors: Lu, Teliang and Wang, Jinchao and Yuan, Xinyuan and Tang, Chenyu and Wang, Xiaolan and He, Fupo and Ye, Jiandong
Journal: Biomaterials Advances (2022): 213120
Authors: Lu, Teliang and Wang, Jinchao and Yuan, Xinyuan and Tang, Chenyu and Wang, Xiaolan and He, Fupo and Ye, Jiandong
Journal: Biomaterials Advances (2022): 213120
Evaluation of Polyacrylonitrile Nonwoven Mats and Silver--Gold Bimetallic Nanoparticle-Decorated Nonwoven Mats for Potential Promotion of Wound Healing In Vitro and In Vivo and Bone Growth In Vitro
Authors: Bai, Meng-Yi and Ku, Fang-Yu and Shyu, Jia-Fwu and Hayashi, Tomohiro and Wu, Chia-Chun
Journal: Polymers (2021): 516
Authors: Bai, Meng-Yi and Ku, Fang-Yu and Shyu, Jia-Fwu and Hayashi, Tomohiro and Wu, Chia-Chun
Journal: Polymers (2021): 516
Evaluation of Polyacrylonitrile Nonwoven Mats and Silver Gold Bimetallic Nanoparticle Decorated Nonwoven Mats for Potential Promotion of Wound Healing In Vitro and In Vivo and Bone Growth In Vitro
Authors: Bai, Meng-Yi and Ku, Fang-Yu and Shyu, Jia-Fwu and Hayashi, Tomohiro and Wu, Chia-Chun
Journal: Polymers (2021): 516
Authors: Bai, Meng-Yi and Ku, Fang-Yu and Shyu, Jia-Fwu and Hayashi, Tomohiro and Wu, Chia-Chun
Journal: Polymers (2021): 516
Calcitonin induces bone formation by increasing expression of Wnt10b in osteoclasts in ovariectomy-induced osteoporotic rats
Authors: Hsiao, Chen-Yuan and Chen, Tien-Hua and Chu, Tzu-Hui and Ting, Yen-Nien and Tsai, Pei-Jiun and Shyu, Jia-Fwu
Journal: Frontiers in Endocrinology (2020): 613
Authors: Hsiao, Chen-Yuan and Chen, Tien-Hua and Chu, Tzu-Hui and Ting, Yen-Nien and Tsai, Pei-Jiun and Shyu, Jia-Fwu
Journal: Frontiers in Endocrinology (2020): 613
References
View all 42 references: Citation Explorer
Velocity distributions of single F-actin trajectories from a fluorescence image series using trajectory reconstruction and optical flow mapping
Authors: von Wegner F, Ober T, Weber C, Schurmann S, Winter R, Friedrich O, Fink RH, Vogel M.
Journal: J Biomed Opt (2008): 54018
Authors: von Wegner F, Ober T, Weber C, Schurmann S, Winter R, Friedrich O, Fink RH, Vogel M.
Journal: J Biomed Opt (2008): 54018
Visualization of F-actin and G-actin equilibrium using fluorescence resonance energy transfer (FRET) in cultured cells and neurons in slices
Authors: Okamoto K, Hayashi Y.
Journal: Nat Protoc (2006): 911
Authors: Okamoto K, Hayashi Y.
Journal: Nat Protoc (2006): 911
The effect of F-actin on the relay helix position of myosin II, as revealed by tryptophan fluorescence, and its implications for mechanochemical coupling
Authors: Conibear PB, Malnasi-Csizmadia A, Bagshaw CR.
Journal: Biochemistry (2004): 15404
Authors: Conibear PB, Malnasi-Csizmadia A, Bagshaw CR.
Journal: Biochemistry (2004): 15404
Analysis of models of F-actin using fluorescence resonance energy transfer spectroscopy
Authors: Moens PD, dos Remedios CG.
Journal: Results Probl Cell Differ (2001): 59
Authors: Moens PD, dos Remedios CG.
Journal: Results Probl Cell Differ (2001): 59
Fluorescence studies of the carboxyl-terminal domain of smooth muscle calponin effects of F-actin and salts
Authors: Bartegi A, Roustan C, Kassab R, Fattoum A.
Journal: Eur J Biochem (1999): 335
Authors: Bartegi A, Roustan C, Kassab R, Fattoum A.
Journal: Eur J Biochem (1999): 335
Microquantification of cellular and in vitro F-actin by rhodamine phalloidin fluorescence enhancement
Authors: Katanaev VL, Wymann MP.
Journal: Anal Biochem (1998): 185
Authors: Katanaev VL, Wymann MP.
Journal: Anal Biochem (1998): 185
A conformational change in F-actin when myosin binds: fluorescence resonance energy transfer detects an increase in the radial coordinate of Cys-374
Authors: Moens PD, dos Remedios CG.
Journal: Biochemistry (1997): 7353
Authors: Moens PD, dos Remedios CG.
Journal: Biochemistry (1997): 7353
Interhead distances in myosin attached to F-actin estimated by fluorescence energy transfer spectroscopy
Authors: Ishiwata S, Miki M, Shin I, Funatsu T, Yasuda K, dos Remedios CG.
Journal: Biophys J (1997): 895
Authors: Ishiwata S, Miki M, Shin I, Funatsu T, Yasuda K, dos Remedios CG.
Journal: Biophys J (1997): 895
Myosin-induced changes in F-actin: fluorescence probing of subdomain 2 by dansyl ethylenediamine attached to Gln-41
Authors: Kim E, Miller CJ, Motoki M, Seguro K, Muhlrad A, Reisler E.
Journal: Biophys J (1996): 1439
Authors: Kim E, Miller CJ, Motoki M, Seguro K, Muhlrad A, Reisler E.
Journal: Biophys J (1996): 1439
Changes in the distribution of F-actin in the fission yeast Schizosaccharomyces pombe by arresting growth in distilled water: correlative studies with fluorescence and electron microscopy
Authors: Kanbe T, Akashi T, Tanaka K.
Journal: J Electron Microsc (Tokyo) (1994): 20
Authors: Kanbe T, Akashi T, Tanaka K.
Journal: J Electron Microsc (Tokyo) (1994): 20
Application notes
Abbreviation of Common Chemical Compounds Related to Peptides
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High-Content High-Throughput Assays and iPSC Neuronal Cultures
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Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin-resistant uterine cancer
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Can Cat#22666 stain mitochondria in dead cells?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Can rhodamines be used to investigate ROS?
Can the endoplasmic reticulum stop working?
Can Cat#22666 stain mitochondria in dead cells?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Can rhodamines be used to investigate ROS?
Can the endoplasmic reticulum stop working?
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