Cell Navigator® F-Actin Labeling Kit *Red Fluorescence*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Absorbance (nm) | 587 |
Correction Factor (260 nm) | 0.05 |
Correction Factor (280 nm) | 0.04 |
Extinction coefficient (cm -1 M -1) | 1800001 |
Excitation (nm) | 588 |
Emission (nm) | 604 |
Quantum yield | 0.531 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12352200 |
Alternative formats
Cell Navigator® F-Actin Labeling Kit *Blue Fluorescence* |
Cell Navigator® F-Actin Labeling Kit *Green Fluorescence* |
Cell Navigator® F-Actin Labeling Kit *Orange Fluorescence* |
Related products
Overview | ![]() ![]() |
See also: Mitochondria, Cytoskeleton Structure & Analysis
Absorbance (nm) 587 | Correction Factor (260 nm) 0.05 | Correction Factor (280 nm) 0.04 | Extinction coefficient (cm -1 M -1) 1800001 | Excitation (nm) 588 | Emission (nm) 604 | Quantum yield 0.531 |
Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to label F-actins of fixed cells in red fluorescence. The kit uses a red fluorescent phalloidin conjugate that is selectively bound to F-actins. This red fluorescent phalloidin conjugate is a high-affinity probe for F-actins. Used at nanomolar concentrations, phallotoxins are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. The labeling protocol is robust, requiring minimal hands-on time. The kit provides all the essential components with an optimized staining protocol.
Platform
Fluorescence microscope
Excitation | Texas Red channel |
Emission | Texas Red channel |
Recommended plate | Black wall/clear bottom |
Components
Example protocol
AT A GLANCE
Protocol summary
- Prepare samples (microplate wells)
- Remove the liquid from the plate
- Add 100 µL/well of iFluor™ 594-Phalloidin working solution
- Stain the cells at RT for 15 to 60 minutes
- Wash the cells
- Examine the specimen under fluorescence microscope at Ex/Em = 594/610 nm (Texas Red channel)
Important notes
Thaw all the components at room temperature before starting the experiment.
PREPARATION OF WORKING SOLUTION
Add 10 μL of iFluor™ 594-Phalloidin (Component A) to 10 mL of Labeling Buffer (Component B) to make 1X iFluor™ 594-Phalloidin working solution. Protect from light. Note: Different cell types might be stained differently. The concentration of iFluor™ 594-Phalloidin working solution should be prepared accordingly.
For guidelines on cell sample preparation, please visit
https://www.aatbio.com/resources/guides/cell-sample-preparation.html
SAMPLE EXPERIMENTAL PROTOCOL
- Perform formaldehyde fixation. Incubate the cells with 3.0 – 4.0% formaldehyde in PBS at room temperature for 10 – 30 minutes. Note: Avoid any methanol containing fixatives since methanol can disrupt actin during the fixation process. The preferred fixative is methanol-free formaldehyde.
- Rinse the fixed cells 2 – 3 times in PBS.
- Optional: Add 0.1% Triton X-100 in PBS into fixed cells for 3 to 5 minutes to increase permeability. Rinse the cells 2 – 3 times in PBS.
- Add 100 µL/well (96-well plate) of iFluor™ 594-Phalloidin working solution into the fixed cells.
- Stain the cells at room temperature for 15 to 60 minutes.
- Rinse cells gently with PBS 2 to 3 times to remove excess dye before plate sealing.
- Image cells using a fluorescence microscope with Texas Red channel (Ex/Em = 594/610 nm).
Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Absorbance (nm) | 587 |
Correction Factor (260 nm) | 0.05 |
Correction Factor (280 nm) | 0.04 |
Extinction coefficient (cm -1 M -1) | 1800001 |
Excitation (nm) | 588 |
Emission (nm) | 604 |
Quantum yield | 0.531 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
Cell Navigator® F-Actin Labeling Kit *Blue Fluorescence* | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
Cell Navigator® F-Actin Labeling Kit *Green Fluorescence* | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
Cell Navigator® F-Actin Labeling Kit *Orange Fluorescence* | 541 | 557 | 1000001 | 0.671 | 0.25 | 0.15 |
Images

Figure 1. Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with Cell Navigator® F-Actin Labeling Kit *Red Fluorescence* in a Costar black 96-well plate. Cells were labeled with iFluor® 594-Phalloidin (Cat#22664, Red) and nuclei stain DAPI (Cat#17507, Blue), respectively. Cell endoplasmic reticulum (ER) was stained with ER Green™ (Cat#22635, Green) before fixation.
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