Cell Navigator® F-Actin Labeling Kit *Red Fluorescence*

Cell Navigator® F-Actin Labeling Kit

Red Fluorescence

Cell Navigator® F-Actin Labeling Kit is designed for selective red fluorescent labeling of filamentous actin (F-actin) in fixed, permeabilized cells.

Selective F-Actin Labeling: Uses iFluor® 594-Phalloidin to bind filamentous actin
Red Fluorescence Detection: Provides excitation and emission maxima of approximately 587 nm and 603 nm, respectively
Convenient Kit Format: Includes fluorescent phalloidin and labeling buffer for 500 tests
Applications: Supports fluorescence imaging of actin filaments and cytoskeletal organization in fixed samples

<p>Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with Cell Navigator® F-Actin Labeling Kit *Red Fluorescence* in a Costar black 96-well plate. Cells were labeled with iFluor® 594-Phalloidin (Cat#22664, Red) and nuclei stain DAPI (Cat#17507, Blue), respectively. Cell endoplasmic reticulum (ER) was stained with ER Green™ (Cat#22635, Green) before fixation.</p>

Protocol

SDS

Catalog	Size	Price	Quantity
22664	500 Tests	Price

Overview

Cell Navigator® F-Actin Labeling Kit uses iFluor® 594-Phalloidin to selectively label F-actin with red fluorescence. The fluorescent phalloidin conjugate binds actin filaments, enabling visualization of the actin cytoskeleton in formaldehyde-fixed, permeabilized cells and tissue sections.

The kit includes iFluor® 594-Phalloidin and labeling buffer, with sufficient reagents for 500 tests. The probe has excitation and emission maxima of approximately 587 nm and 603 nm, respectively, and can be imaged using a Texas Red-compatible filter set. Cell Navigator® F-Actin Labeling Kit supports fluorescence microscopy studies of actin filament distribution and cytoskeletal organization.

Citations

View all 32 citations: Citation Explorer

PANX2 Suppresses Lung Adenocarcinoma Progression by Inducing Disulfidptosis and Enhancing Antitumor Immunity

Authors:

Chen, Yi and Liu, Zhao-Yu and Qu, Gao-Wen and Zeng, Run-Hao and Yao, Wen-Xia and Zhou, Yong-Bing and Liang, Xue and Lai, Yu-Xiong

Journal:

Advanced Science (2026): e75662

Orchestrated Adhesive Anisotropic/Anti-Adhesive Isotropic Janus Conductive Patch Reverses Ventricular Remodeling via Synchronized Electrophysiological Reconstitution and Mitochondrial-Targeted Salvage

Authors:

Chen, Shuhui and Zhang, Xin and Luo, Guangbing and Zhao, Qiuyuan and shen, Jie and Zhan, Jiamian and Xie, Qing and Hua, Shaofeng and Hou, Honghao and He, Yutong and others,

Journal:

Advanced Functional Materials (2025): e19577

Progerin regulates actin cytoskeletal remodeling and inhibits EMT and metastasis in triple-negative breast cancer cells

Authors:

Huang, Xinxian and Luo, Weizhao and Liu, Weixian and Liu, Xinguang and Chen, Weichun

Journal:

International Journal of Oncology (2025): 1--13

Engineered Extracellular Vesicles Derived from Juvenile Mice Enhance Mitochondrial Function in the Aging Bone Microenvironment and Achieve Rejuvenation

Authors:

Zheng, Jiaqian and Ren, Yipeng and Ke, Junhua and Zhu, Guanglin and Wang, Zhen and Shi, Xuetao and Wang, Yingjun

Journal:

ACS nano (2025)

A functional cardiac patch promotes cardiac repair by modulating the CCR2- cardiac-resident macrophage niche and their cell crosstalk

Authors:

Ding, Chengbin and Tang, Guofeng and Sun, Yan and Fu, Xiaodong and Tian, Ye and Zhan, Jiamian and Zhang, Songtao and Xing, Xianglong and Liu, Jianing and Qiu, Xiaozhong and others,

Journal:

Cell Reports Medicine (2025)

References

View all 42 references: Citation Explorer

Velocity distributions of single F-actin trajectories from a fluorescence image series using trajectory reconstruction and optical flow mapping

Authors:

von Wegner F, Ober T, Weber C, Schurmann S, Winter R, Friedrich O, Fink RH, Vogel M.

Journal:

J Biomed Opt (2008): 54018

Visualization of F-actin and G-actin equilibrium using fluorescence resonance energy transfer (FRET) in cultured cells and neurons in slices

Authors:

Okamoto K, Hayashi Y.

Journal:

Nat Protoc (2006): 911

The effect of F-actin on the relay helix position of myosin II, as revealed by tryptophan fluorescence, and its implications for mechanochemical coupling

Authors:

Conibear PB, Malnasi-Csizmadia A, Bagshaw CR.

Journal:

Biochemistry (2004): 15404

Analysis of models of F-actin using fluorescence resonance energy transfer spectroscopy

Authors:

Moens PD, dos Remedios CG.

Journal:

Results Probl Cell Differ (2001): 59

Fluorescence studies of the carboxyl-terminal domain of smooth muscle calponin effects of F-actin and salts

Authors:

Bartegi A, Roustan C, Kassab R, Fattoum A.

Journal:

Eur J Biochem (1999): 335

Spectral properties

Absorbance (nm)	587
Correction factor (260 nm)	0.05
Correction factor (280 nm)	0.04
Extinction coefficient (cm ^-1 M ^-1)	200000 ¹
Excitation (nm)	587
Emission (nm)	603
Quantum yield	0.53 ¹

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microscope
Excitation	Texas Red channel
Emission	Texas Red channel
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 28, 2026

Component A: iFluor® 594-Phalloidin	1 vial (50 µL)
Component B: Labeling Buffer	1 bottle (50 mL)