Cell Navigator™ Flow Cytometric Exosome Staining Kit
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.92 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Refrigerated (2-8 °C); Minimize light exposure |
UNSPSC | 12352200 |
Related products
Overview | SDSProtocol |
See also: Exosomes
Correction Factor (280 nm) 0.35 | Extinction coefficient (cm -1 M -1) 73000 | Excitation (nm) 491 | Emission (nm) 516 | Quantum yield 0.92 |
Exosomes, nanoscale (30-150 nm) membrane-bound vesicles secreted by cells, are central to intercellular communication. They transfer proteins, lipids, and nucleic acids, including RNA species, between cells, playing a pivotal role in physiological and pathological processes such as immune modulation, disease progression, and genetic information exchange. This makes exosomes key targets in therapeutic and diagnostic research. However, challenges such as dye aggregation and the absence of bright, specific probes have hindered the reliable detection and analysis of exosomes. The Cell Navigator™ Flow Cytometric Exosome Staining Kit addresses these challenges by enabling the detection of CD9-positive exosomes in purified or bead-bound samples. It utilizes a fluorescent dye-conjugated CD9 marker for targeted visualization, providing a straightforward staining protocol for rapid and accurate exosome detection in diverse sample matrices, including cell culture supernatants, biological fluids, and isolated exosome preparations. To ensure effective detection and analysis, it is essential to enrich the cell culture media for exosomes before staining. This can be achieved through efficient methods such as the ReadiPrep™ Exosome Isolation Kit (Cat No. 60204) or traditional ultracentrifugation.
Platform
Flow cytometer
Excitation | 488 nm Laser |
Emission | 530/30 nm Filter |
Instrument specification(s) | FITC Channel |
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
Staining of Exosomes
Isolate exosomes from cell culture medium using the ReadiPrep™ Exosome Isolation Kit (Cat# 60204) or your method of choice.
Add 5 uL of Exosome CD9 Staining Reagent into 100 uL of exosome solution.
Incubate the mix for 1 to 3 hours in the dark at room temperature.
Perform flow cytometry with FITC filter set (488 nm laser with 530/30 nm filter).
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.92 |
Images
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?