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Cell Navigator™ Flow Cytometric Exosome Staining Kit
Exosomes, nanoscale (30-150 nm) membrane-bound vesicles secreted by cells, are central to intercellular communication. They transfer proteins, lipids, and nucleic acids, including RNA species, between cells, playing a pivotal role in physiological and pathological processes such as immune modulation, disease progression, and genetic information exchange. This makes exosomes key targets in therapeutic and diagnostic research. However, challenges such as dye aggregation and the absence of bright, specific probes have hindered the reliable detection and analysis of exosomes. The Cell Navigator™ Flow Cytometric Exosome Staining Kit addresses these challenges by enabling the detection of CD9-positive exosomes in purified or bead-bound samples. It utilizes a fluorescent dye-conjugated CD9 marker for targeted visualization, providing a straightforward staining protocol for rapid and accurate exosome detection in diverse sample matrices, including cell culture supernatants, biological fluids, and isolated exosome preparations. To ensure effective detection and analysis, it is essential to enrich the cell culture media for exosomes before staining. This can be achieved through efficient methods such as the ReadiPrep™ Exosome Isolation Kit (Cat No. 60204) or traditional ultracentrifugation.
Exosomes from 100,000 HeLa cells (cultured for 16 hours in 10 mL serum-free medium) were isolated using ReadiPrep™ Exosome Isolation Kit (Cat# 60204) and detected using the Cell Navigator™ Flow Cytometric Exosome Staining Kit (Cat# 22426).
Exosomes from 100,000 HeLa cells (cultured for 16 hours in 10 mL serum-free medium) were isolated using ReadiPrep™ Exosome Isolation Kit (Cat# 60204) and detected using the Cell Navigator™ Flow Cytometric Exosome Staining Kit (Cat# 22426).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22426100 Tests
Price
 
Spectral properties
Correction factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)
73000
Excitation (nm)491
Emission (nm)516
Quantum yield
0.92
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Flow cytometer
Excitation488 nm Laser
Emission530/30 nm Filter
Instrument specification(s)FITC Channel
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Page updated on October 11, 2025