ReadiPrep™ Exosome Isolation Kit
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Refrigerated (2-8 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
To ensure isolated exosomes originate from the cells, culture cells with exosome-depleted fetal bovine serum (FBS) or no FBS. Normal FBS contains a significantly higher amount of exosomes and may give false positive results. It is important to note that this kit has been tested only for exosome isolation from cell culture media.
Grow cells for a desired time period.
Harvest cell culture medium.
Centrifuge the cell culture medium at 2000 x g for 30 minutes to remove cell debris and remaining floating cells.
Transfer the supernatant into a new tube without disturbing the pellet.
Transfer the necessary volume of supernatant (prepared as described in "Preparation of cell samples") into a fresh tube, and then add an equal volume of Exosome Isolation Buffer. For instance, if you are isolating from 10 mL of cell culture medium, you should add 10 mL of Exosome Isolation Buffer.
Mix it well and incubate samples at 2 ℃ to 8 ℃ overnight.
After incubation, centrifuge the samples at 10,000 x g for 1 hour at 2 ℃ to 8 ℃.
Remove the supernatant and discard it. Exosomes should be in the pellet at the bottom of the tube.
Note: The pellet may not be visible in most cases.
Resuspend the pellet in 1X PBS with the desired volume. For example, if the starting cell culture media volume is 10 mL, then the resuspension volume should be 100 µL–1 mL.
Store the isolated exosomes at 2 ℃ to 8 ℃ for up to 1 week or at ≤ 20 °C for long-term storage.
Images
References
Authors: Ansari, Farshid Jaberi and Tafti, Hossein Ahmadi and Amanzadeh, Amir and Rabbani, Shahram and Shokrgozar, Mohammad Ali and Heidari, Reza and Behroozi, Javad and Eyni, Hossein and Uversky, Vladimir N and Ghanbari, Hossein
Journal: Biochemistry and biophysics reports (2024): 101668
Authors: Teflischi Gharavi, Abdulwahab and Niknejad, Azadeh and Irian, Saeed and Rahimi, Amirabbas and Salimi, Rahimi
Journal: Iranian biomedical journal (2024)
Authors: Kim, Minse and Song, Chi-Yeon and Lee, Jin Sil and Ahn, Yu-Rim and Choi, Jaewon and Lee, Sang Hoon and Shin, SoJin and Na, Hee Jun and Kim, Hyun-Ouk
Journal: Advanced healthcare materials (2024): e2303782
Authors: Gorgzadeh, Amirsasan and Nazari, Ahmad and Ali Ehsan Ismaeel, Adnan and Safarzadeh, Diba and Hassan, Jawad A K and Mohammadzadehsaliani, Saman and Kheradjoo, Hadis and Yasamineh, Pooneh and Yasamineh, Saman
Journal: Virology journal (2024): 34
Authors: Ramnauth, Natasha and Neubarth, Elise and Makler-Disatham, Amy and Sher, Mazhar and Soini, Steven and Merk, Vivian and Asghar, Waseem
Journal: Sensors (Basel, Switzerland) (2023)
Authors: Ciftci, Eda and Bozbeyoglu, Naz and Gursel, Ihsan and Korkusuz, Feza and Bakan Misirlioglu, Feray and Korkusuz, Petek
Journal: PloS one (2023): e0282238
Authors: Mimmi, Selena and Zimbo, Anna Maria and Rotundo, Salvatore and Cione, Erika and Nisticò, Nancy and Aloisio, Annamaria and Maisano, Domenico and Tolomeo, Anna Maria and Dattilo, Vincenzo and Lionello, Rosaria and Fioravanti, Antonella and Di Loria, Antonio and Quirino, Angela and Marascio, Nadia and Russo, Alessandro and Trecarichi, Enrico Maria and Matera, Giovanni and Quinto, Ileana and Torti, Carlo and Iaccino, Enrico
Journal: Clinical chemistry and laboratory medicine (2023): 1518-1524
Authors: Mahgoub, Elham O and Abdella, Galal M
Journal: Journal of cancer research and clinical oncology (2023): 7505-7514
Authors: D'Agostino, Erika and Muro, Annamaria and Sgueglia, Giulia and Massaro, Crescenzo and Dell'Aversana, Carmela and Altucci, Lucia
Journal: Methods in molecular biology (Clifton, N.J.) (2023): C1
Authors: Chen, Yulin and Gao, Dan and Zhu, Qingyun and Chu, Bizhu and Peng, Jie and Wang, Jian and Liu, Liping and Jiang, Yuyang
Journal: The Analyst (2023): 2387-2394
Application notes
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?