Exosomes are nanoscale (30-150 nm), membrane-bound vesicles secreted by cells into the extracellular space, functioning as pivotal mediators in intercellular communication. They facilitate the transfer of proteins, lipids, and nucleic acids, including RNA species, between cells. This vesicular transport mechanism is implicated in various physiological and pathological processes, including immune modulation, disease progression, and genetic information exchange, making exosomes a focal point in both therapeutic and diagnostic research. The isolation of exosomes typically involves complex and time-consuming processes, such as ultracentrifugation, which can be challenging to execute. The ReadiPrep™ Exosome Isolation Kit offers a streamlined and effective approach to isolating intact exosomes from diverse biological samples, including cell culture media and serum. The principle behind this kit is based on the selective precipitation of exosomes by inducing the removal of water molecules from the solution. Following a brief, low-speed centrifugation step, the exosomes are precipitated and can subsequently be isolated through standard centrifugation. The isolated exosomal pellet is then resuspended in PBS or an equivalent buffer, rendering the exosomes suitable for further downstream analyses or purification via affinity-based methods.