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Cell Navigator® Fluorimetric Lipid Droplet Assay Kit *Blue Fluorescence*
Product key features
Cell Navigator® Fluorimetric Lipid Droplet Assay Kit is ideal for detecting and analyzing lipid droplets in diverse research applications.
- Minimal background: High specificity for lipid droplets with minimal background noise
- Versatile applications: Compatible with fluorescence microscopy, flow cytometry, and fluorescence microplate readers
- Comparable alternative: Provides lower background and higher signal specificity comparable to LipidTOX™ neutral lipid stains from Thermo Fisher
- Non-toxic: Suitable for live cell imaging, offering a safe method to study lipid droplets in real time
Product description
Cell Navigator® Fluorimetric Lipid Droplet Assay Kit (Blue Fluorescence) is designed for the sensitive and specific detection of lipid droplets (adiposomes) in live or fixed cells. The kit features Droplite™ Blue, a lipophilic dye that exhibits bright blue fluorescence in lipid-rich environments with minimal background in aqueous media. Lipid droplets are essential organelles that store neutral lipids, regulate cellular lipid metabolism, and participate in processes such as energy production, signaling, and membrane maintenance.
This kit enables accurate visualization of lipid droplets in a wide range of biological and pathological studies, including metabolic disorders, liver dysfunction, and cardiovascular diseases. Droplite™ Blue is compatible with fluorescence microscopy, flow cytometry, and fluorescence microplate readers using standard DAPI filter sets. It is suitable for live cell imaging and provides a non-toxic, high-sensitivity method for assessing lipid accumulation.
Example protocol
AT A GLANCE
Prepare cells with test compounds.
Add Droplite™ Blue working solution.
Incubate at room temperature or 37°C for 10 to 30 minutes.
Read fluorescence intensity with a fluorescence microscope using a DAPI filter set.
This protocol is our recommended guideline for live cells, but it can be adjusted to meet your specific requirements. Since Droplite™ Blue exhibits minimal fluorescence in aqueous media, removing the growth medium and staining solution after staining is optional. Stained cells can be fixed with 3–4% formaldehyde. Alternatively, prefixed cells (fixed with 3–4% formaldehyde) can also be stained using the Droplite™ Blue staining solution.
CELL PREPARATION
For guidelines on cell sample preparation, please visit:
https://www.aatbio.com/resources/guides/cell-sample-preparation.html
PREPARATION OF WORKING SOLUTION
To prepare the Droplite™ Blue working solution, dilute 5 µL of the Droplite™ Blue (Component A) in 1 mL of Staining Buffer (Component B).
Note: 50 µL of Droplite™ Blue (Component A) is enough for one 96-well plate. Protect the solution from light. The optimal concentration of Droplite™ Blue may vary depending on the application. Adjust staining conditions based on the cell type and the permeability of cells or tissues to the probe.
SAMPLE EXPERIMENTAL PROTOCOL
Grow cells either in a 96-well black wall/clear bottom plate (100 µL/well/96-well) or on cover-slips inside a petri dish filled with the appropriate culture medium.
Gently aspirate the culture medium and add equal volume (such as 100 µL/well/96-well plate) of the Droplite™ Blue staining solution.
Incubate the cells in a 37°C, 5% CO2 incubator for 10 - 30 minutes.
Remove Droplite™ Blue working solution (Optional).
Measure fluorescence at Ex/Em = 350/450 nm with a microplate reader or observe the cells using a fluorescence microscope equipped with a DAPI filter set.
Centrifuge the cells at 1000 rpm for 5 minutes to get 1 - 5 × 105 cells per tube.
Resuspend cells in 500 µL of Droplite™ Blue working solution.
Incubate at room temperature or 37°C for 10 to 30 min, protected from light.
Centrifuge to remove the Droplite™ Blue working solution, and resuspend cells in 500 µL of pre-warmed medium or buffer of your choice to get 1 - 5 × 105 cells per tube (Optional).
Monitor the fluorescence increase using a fluorescence microscope equipped with a DAPI filter set.
Spectrum
References
Authors: Chen, Xiuqin and Chen, Guizhi and Dong, Shiqing and Qiu, Liting and Qiu, Ruoyi and Han, Xiangyu and Wang, Zihui and Wang, Kun and Peng, Yiru
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2025): 125012
Authors: Margherita, Cortini and Elizabeta, Ilieva and Stefania, Massari and Giuliano, Bettini and Sofia, Avnet and Nicola, Baldini
Journal: Biochimica et biophysica acta. Molecular basis of disease (2025): 167576
Authors: Vardar, Umay Sevgi and Konings, Gijs and Yang, Jack and Sagis, Leonard M C and Bitter, Johannes H and Nikiforidis, Constantinos V
Journal: Journal of colloid and interface science (2025): 1077-1086
Authors: Dasso, Marina Ercilia and Centola, Cecilia Lucia and Galardo, Maria Noel and Riera, Maria Fernanda and Meroni, Silvina Beatriz
Journal: Molecular and cellular endocrinology (2025): 112403
Authors: Cheng, Yan and Jung, Jaekeun and Guo, Liyang and Shuboni-Mulligan, Dorela D and Chen, Jian-Fu and Hu, Wenhui and Guo, Ming-Lei
Journal: Brain, behavior, and immunity (2025): 108-122
Safety data sheet