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Cell Navigator® Fluorimetric Lipid Droplet Assay Kit
Red Fluorescence
Lipid droplets, also referred to as lipid bodies, oil bodies or adiposomes, are lipid-rich cellular organelles that regulate the storage and hydrolysis of neutral lipids. They also serve as a reservoir of lipid source for many important biological processes such as fatty acid and cellular cholesterol for energy and membrane formation and maintenance. Abnormal accumulation of the cytoplasmic lipid droplets occurs in a variety of pathological conditions and can be an indicator of metabolic deficiency or pathogenesis. AAT Bioquest's Cell Navigator® Fluorimetric Lipid Droplet Assay Kit is a robust tool that could quantitatively measure lipid droplet accumulation. Droplite™ Red is used in the kit for lipophilic stain. Droplite™ Red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. It is an excellent vital stain for the detection of intracellular lipid droplets with fluorescence microscopy, flow cytometry or fluorescence microplate reader. The red fluorescence signal could be read observed using the filter set of TRITC.
<p>Fluorescence images of intracellular lipid droplets in control (Left) and Oleic Acid treated HeLa cells (Right) using Cell Navigator® Lipid Droplets Fluorescence Assay Kit. HeLa cells were incubated with 300 uM of Oleic Acid for 24 hours to induce intracellular lipid droplets formation. After washing with PBS, the cells were labeled with 1X Droplite&trade; Red and Hoechst 33342 &nbsp;(Cat#17533).</p>
<p>Fluorescence images of intracellular lipid droplets in control (Left) and Oleic Acid treated HeLa cells (Right) using Cell Navigator® Lipid Droplets Fluorescence Assay Kit. HeLa cells were incubated with 300 uM of Oleic Acid for 24 hours to induce intracellular lipid droplets formation. After washing with PBS, the cells were labeled with 1X Droplite&trade; Red and Hoechst 33342 &nbsp;(Cat#17533).</p>
protocol
Protocol
sds
SDS
COO
COA
CatalogSize
Price
Quantity
22735200 Tests
Price
 
Spectral properties
Absorbance (nm)560
Extinction coefficient (cm -1 M -1)
38000
Excitation (nm)559
Emission (nm)635
Quantum yield
0.7000
1
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationTRITC filter set
EmissionTRITC filter set
Recommended plateBlack wall/clear bottom
Fluorescence microplate reader
Excitation550 nm
Emission640 nm
Cutoff610 nm
Recommended plateSolid black
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Page updated on September 25, 2025