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Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit
Green Fluorescence
The endoplasmic reticulum (ER) is a type of organelle in the cells of eukaryotic organisms that forms an interconnected network of flattened, membrane-enclosed sacs or tube-like structures known as cisternae. The membranes of the ER are continuous with the outer nuclear membrane. ER occurs in most types of eukaryotic cells, but is absent from red blood cells and spermatozoa. This Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit uses our ER Tracer™ Green as an ER marker. ER Tracer™ Green stain is a cell-permeant fluorescent dye that is highly selective for ER. This stain consists of a green fluorescent dye and ER binder that selectively bind to ER in most of cell types. For some cells, ER Tracer™ Green may not selectively bind to ER. ER Tracer™ Green has spectral properties essentially identical to FITC, making this kit convenient with the FITC filter set. ER Tracer™ is good replacement for ER Tracker (trademark ThermoFisher).
Fluorescence images of endoplasmic reticulum (ER) staining in HeLa cells cultured in a 96-well black-wall clear-bottom plate using fluorescence microscope with a FITC filter set. Left: Live cells were stained with ER-selective probe ER Tracer™ Green (Cat#22635, Green), mitochondria dye MitoLite™ Red FX600 (Cat#22677, Red) and nuclei stain Hoechst 33342 (Cat#17530, Blue). Right: Live cells stained with ER Tracer™ Green (Cat#22635, Green) were fixed with 4% formaldehyde, and labeled with F-actin dye iFluor® 594-Phalloidin (Cat#23122, Red) and nuclei stain DAPI (Cat#17507, Blue).
Fluorescence images of endoplasmic reticulum (ER) staining in HeLa cells cultured in a 96-well black-wall clear-bottom plate using fluorescence microscope with a FITC filter set. Left: Live cells were stained with ER-selective probe ER Tracer™ Green (Cat#22635, Green), mitochondria dye MitoLite™ Red FX600 (Cat#22677, Red) and nuclei stain Hoechst 33342 (Cat#17530, Blue). Right: Live cells stained with ER Tracer™ Green (Cat#22635, Green) were fixed with 4% formaldehyde, and labeled with F-actin dye iFluor® 594-Phalloidin (Cat#23122, Red) and nuclei stain DAPI (Cat#17507, Blue).
protocol
Protocol
sds
SDS
COO
COA
CatalogSize
Price
Quantity
22635100 Tests
Price
 
Spectral properties
Excitation (nm)503
Emission (nm)511
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
Excitation490 nm
Emission520 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)FITC filter
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Page updated on September 25, 2025