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Cell Navigator® Live Cell RNA Imaging Kit
Green Fluorescence
Detecting and imaging RNA molecules in living cells is extremely important for a wide variety of molecular biology procedures including physical transportation, interpretation of genetic information, regulation of gene expression and some essential bio-catalytic roles. The major challenge to stain RNA in living cells is the interferences caused by DNA. In order to address the difficulty, a novel green fluorogenic dye was developed as a RNA-selective probe. AAT Bioquest's Cell Navigator® Live Cell RNA Imaging Kit includes StrandBrite™ RNA Green as it specifically binds RNA in cells. Compared to commercial SYTO™ RNA Select dye for RNA staining in vivo, StrandBrite™ RNA Green shows brighter signal and much better selectivity to RNA. In addition, this kit can stain RNA in both living cells and fixed cells.
Fluorescence images of RNA staining in HeLa cells. (A) Live cells were stained using Cell Navigator® Live Cell RNA Imaging Kit (Green, Cat#22630) and counter-stained with Hoechst 33342 (Blue, Cat#17530). (B) Cells fixed in methanol were stained using the same kit. (C) After staining, fixed HeLa cells were incubated with 0.5 mg/mL RNase at 37 ºC for 1 hour. Image of RNase digest test indicates the high selectivity of StrandBrite™ RNA Green. The green fluorescence signal were measured using a fluorescence microscope with a FITC filter.
Fluorescence images of RNA staining in HeLa cells. (A) Live cells were stained using Cell Navigator® Live Cell RNA Imaging Kit (Green, Cat#22630) and counter-stained with Hoechst 33342 (Blue, Cat#17530). (B) Cells fixed in methanol were stained using the same kit. (C) After staining, fixed HeLa cells were incubated with 0.5 mg/mL RNase at 37 ºC for 1 hour. Image of RNase digest test indicates the high selectivity of StrandBrite™ RNA Green. The green fluorescence signal were measured using a fluorescence microscope with a FITC filter.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22630100 Tests
Price
 
Spectral properties
Excitation (nm)509
Emission (nm)527
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C)
UNSPSC12352200
Instrument settings
Fluorescence microscope
Excitation490 nm
Emission520 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)FITC filter set
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Page updated on October 7, 2025