Cell Navigator® Live Cell RNA Imaging Kit *Green Fluorescence*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Spectral properties
| Excitation (nm) | 509 |
| Emission (nm) | 527 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C) |
| UNSPSC | 12352200 |
Related products
| Overview |  SDSProtocol |
Excitation (nm) 509 | Emission (nm) 527 |
Detecting and imaging RNA molecules in living cells is extremely important for a wide variety of molecular biology procedures including physical transportation, interpretation of genetic information, regulation of gene expression and some essential bio-catalytic roles. The major challenge to stain RNA in living cells is the interferences caused by DNA. In order to address the difficulty, a novel green fluorogenic dye was developed as a RNA-selective probe. AAT Bioquest's Cell Navigator® Live Cell RNA Imaging Kit includes StrandBrite™ RNA Green as it specifically binds RNA in cells. Compared to commercial SYTO™ RNA Select dye for RNA staining in vivo, StrandBrite™ RNA Green shows brighter signal and much better selectivity to RNA. In addition, this kit can stain RNA in both living cells and fixed cells.
Platform
Fluorescence microscope
| Excitation | 490 nm |
| Emission | 520 nm |
| Recommended plate | Black wall/clear bottom |
| Instrument specification(s) | FITC filter set |
Components
Example protocol
AT A GLANCE
Protocol summary
- Prepare cells
- Add StrandBrite™ RNA Green working solution
- Incubate for 30 - 60 minutes
- Analyze the cells under fluorescence microscope at Ex/Em = 490/520 nm (FITC filter set)
Important notes
Thaw all the components at room temperature before starting the experiment.
SAMPLE EXPERIMENTAL PROTOCOL
- Culture cells to a density optimum for imaging according to your specific induction protocol (about 1 - 2 × 104 cells/well/96-well plate).
- For living cells: Incubate cells with StrandBrite™ RNA Green (Component A) diluted 400X in medium or live cell staining buffer (Component B) at room temperature for 30 - 60 minutes (100 µL/well). Note: 25 µL of StrandBrite™ RNA Green (Component A) is enough for one 96-well plate. Protect from light and avoid repeated freeze-thaw cycles. The appropriate incubation time depends on the individual cell type and cell concentration used. Optimize the incubation time for each experiment. See figure 1 for details.
- For fixed cells: Fix cells with pure methanol for 1 minute at room temperature, then wash with PBS. Immerse cells in 1% Triton-100 for 2 minutes, then wash with PBS twice. Incubate cells with StrandBrite™ RNA Green (Component A) at the concentration of 1X in PBS at room temperature for 15 - 30 minutes. Note: It is recommended to increase either the labeling concentration or the incubation time to allow the dye to accumulate if the cells do not appear to be sufficiently stained. See figure 1 for details.
- (Optional) Wash the cells with PBS for 1 - 2 times, add 100 µL PBS to each well.
- Monitor fluorescence intensity with fluorescence microscope at Ex/Em = 490/520 nm (FITC channel).
Images
Figure 1. Fluorescence images of RNA staining in HeLa cells. (A) Live cells were stained using Cell Navigator® Live Cell RNA Imaging Kit (Green, Cat#22630) and counter-stained with Hoechst 33342 (Blue, Cat#17530). (B) Cells fixed in methanol were stained using the same kit. (C) After staining, fixed HeLa cells were incubated with 0.5 mg/mL RNase at 37 ºC for 1 hour. Image of RNase digest test indicates the high selectivity of StrandBrite™ RNA Green. The green fluorescence signal were measured using a fluorescence microscope with a FITC filter.
Citations
View all 1 citations: Citation Explorer
Poly (ADP-ribose) polymerase 1 (PARP1) inhibition promotes pulmonary metastasis of osteosarcoma by boosting ezrin phosphorylation
Authors: Li, Fangfei and Wu, Xiaoqiu and Fu, Xuekun and Liu, Jin and Song, Wangze and Xiao, Gary Guishan and Lu, Aiping and Zhang, Ge
Journal: International Journal of Biological Sciences (2022): 1238
Authors: Li, Fangfei and Wu, Xiaoqiu and Fu, Xuekun and Liu, Jin and Song, Wangze and Xiao, Gary Guishan and Lu, Aiping and Zhang, Ge
Journal: International Journal of Biological Sciences (2022): 1238