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Cell Navigator® Lysosome Staining Kit
Green Fluorescence
Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc. The selective labeling of live cell compartments provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to label lysosomes of live cells in green fluorescence. The kit uses a proprietary lysotropic dye that selectively accumulates in lysosomes probably vial the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly increases its selectivity for lysosomes. The labeling protocol is robust, requiring minimal hands-on time. It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit provides all the essential components with an optimized cell-labeling protocol. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
Images of HeLa cells stained with A: AAT’s LysoBrite™ Green, B: Invitrogen’s LysoTracker® Green DND-26 in a Costar black wall/clear bottom 96-well plate. Samples were continuously illuminated for 120 seconds, and the signals were compared before and after the exposure by using a Keyence fluorescence microscope.
Images of HeLa cells stained with A: AAT’s LysoBrite™ Green, B: Invitrogen’s LysoTracker® Green DND-26 in a Costar black wall/clear bottom 96-well plate. Samples were continuously illuminated for 120 seconds, and the signals were compared before and after the exposure by using a Keyence fluorescence microscope.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22656500 Tests
Price
 
Spectral properties
Excitation (nm)501
Emission (nm)510
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationFITC filter
EmissionFITC filter
Recommended plateBlack wall/clear bottom
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Page updated on October 12, 2025