Cell Navigator® Lysosome Staining Kit *Green Fluorescence*
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Excitation (nm) | 501 |
Emission (nm) | 510 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12352200 |
Overview | ![]() ![]() |
Excitation (nm) 501 | Emission (nm) 510 |
Platform
Fluorescence microscope
Excitation | FITC filter |
Emission | FITC filter |
Recommended plate | Black wall/clear bottom |
Components
Example protocol
AT A GLANCE
Protocol summary
- Prepare cells
- Add LysoBrite™ Green working solution
- Incubate at 37°C for 30 minutes to 2 hours
- Analyze the cells under fluorescence microscope at Ex/Em = 490/525 nm (FITC filter set)
Important notes
Thaw all the kit components at room temperature before starting the experiment.
PREPARATION OF WORKING SOLUTION
Add 20 µL of 500X LysoBrite™ Green stock solution (Component A) to 10 mL of Live Cell Staining Buffer (Component B) to make LysoBrite™ Green working solution. Protect from light. Note: 20 µL of 500X LysoBrite™ Green (Component A) is enough for one 96-well plate. The optimal concentration of the fluorescent lysosome indicator varies depending on the specific application. The staining conditions may be modified according to the particular cell type and the permeability of the cells or tissues to the probe.
For guidelines on cell sample preparation, please visit
https://www.aatbio.com/resources/guides/cell-sample-preparation.html
SAMPLE EXPERIMENTAL PROTOCOL
For adherent cells:
- Grow cells either in a 96-well black wall/clear bottom plate (100 µL/well/96-well plate) or on cover-slips inside a petri dish filled with the appropriate culture medium. When cells reach the desired confluence, add equal volume of LysoBrite™ Green working solution.
- Incubate the cells in a 37°C, 5% CO2 incubator for 30 minutes to 2 hours.
- Observe the cells using a fluorescence microscope with FITC filter set (Ex/Em = 490/525 nm). Note: It is recommended to increase either the labeling concentration or the incubation time to allow the dye to accumulate if the cells do not appear to be sufficiently stained.
For suspension cells:
- Centrifuge the cells at 1000 rpm for 5 minutes to obtain a cell pellet and aspirate the supernatant.
- Resuspend the cell pellet gently in pre-warmed growth medium, and then add equal volume of LysoBrite™ Green working solution.
- Incubate the cells in a 37°C, 5% CO2 incubator for 30 minutes to 2 hours.
- Observe the cells using a fluorescence microscope with FITC filter set (Ex/Em = 490/525 nm). Note: It is recommended to increase either the labeling concentration or the incubation time to allow the dye to accumulate if the cells do not appear to be sufficiently stained. Suspension cells may be attached to cover-slips that have been treated with BD Cell-Tak® (BD Biosciences) and stained as adherent cells.
Product Family
Images

Citations
Authors: Ben-Akiva, Elana and Karlsson, Johan and Hemmati, Shayan and Yu, Hongzhe and Tzeng, Stephany Y and Pardoll, Drew M and Green, Jordan J
Journal: Proceedings of the National Academy of Sciences (2023): e2301606120
Authors: McGraw, Erin and Roberts, Jonathan D and Kunte, Nitish and Westerfield, Matthew and Streety, Xavier and Held, David and Avila, L Adriana
Journal: ACS omega (2022): 10933--10943
Authors: Malina, Tomáš and Poláková, Katerina and Skopalík, Josef and Milotová, Vera and Holá, Katerina and Havrdová, Markéta and Tománková, Katerina Barton and Cmiel, Vratislav and Sefc, Ludek and Zboril, Radek
Journal: Carbon (2019)
Authors: Mu, Yuzhi and Wu, Guangsheng and Su, Chang and Dong, Yao and Zhang, Kaichao and Li, Jing and Sun, Xiaojie and Li, Yang and Chen, Xiguang and Feng, Chao
Journal: Carbohydrate Polymers (2019): 115072
Authors: Martin, Victor and Ribeiro, Isabel AC and Alves, Marta M and Gon{\c{c}}alves, L{\'\i}dia and Almeida, Ant{\'o}nio J and Grenho, Liliana and Fernandes, Maria H and Santos, Catarina F and Gomes, Pedro S and Bettencourt, Ana F
Journal: International journal of pharmaceutics (2019): 118821
Authors: Lechevallier, S{\'e}verine and Mauricot, Robert and Gros-Dagnac, H{\'e}l{\`e}ne and Chevreux, Sylviane and Lemercier, Gilles and Phonesouk, Erick and Golzio, Muriel and Verelst, Marc
Journal: ChemPlusChem (2017): 770--777
Authors: Lechevallier, Séverine and Mauricot, Robert and Gros-Dagnac, Hélène and Chevreux, Sylviane and Lemercier, Gilles and Phonesouk, Erick and Golzio, Muriel and Verelst, Marc
Journal: ChemPlusChem (2017)
Authors: Wilson, David R and Routkevitch, Denis and Rui, Yuan and Mosenia, Arman and Wahlin, Karl J and Quinones-Hinojosa, Alfredo and Zack, Donald J and Green, Jordan J
Journal: Molecular Therapy (2017)
Authors: Cmiel, Vratislav and Skopalik, Josef and Polakova, Katerina and Solar, Jan and Havrdova, Marketa and Milde, David and Justan, Ivan and Magro, {cmiel2016rhodamine, title={Rhodamine bound maghemite as a long-term dual imaging nanoprobe of adipose tissue-derived mesenchymal stromal cells
Journal: European Biophysics Journal (2016): 1--12
Authors: Paris, Juan L and de la Torre, Paz and Manzano, Miguel and Cabanas, M Victoria and Flores, Ana I and Vallet-Regí, María
Journal: Acta biomaterialia (2016): 275--282
References
Authors: Yabuki A, Suzuki S, Matsumoto M, Nishinakagawa H.
Journal: Histol Histopathol (2002): 1017
Authors: Ikeda K, Akiyama H, Arai T, Kondo H, Haga C, Iritani S, Tsuchiya K.
Journal: Neurosci Lett (1998): 113
Authors: Mozhenok TP, Rpozanov Iu M, Solov'eva LV, Braun AD, Bulychev AG.
Journal: Tsitologiia (1992): 84
Authors: Rungby J, Danscher G, Christensen M, Ellermann-Eriksen S, Mogensen SC.
Journal: Histochemistry (1990): 109
Authors: Shau H, Dawson JR.
Journal: J Immunol (1985): 137
Authors: Shau H, Dawson JR.
Journal: Immunology (1984): 745
Authors: Grabarczyk M, Przybyszewski WM, Kwiatkowska J, Sitarska E, Malec J.
Journal: Neoplasma (1983): 541
Authors: Borisov AB, Bulychev AG, Rumiantsev PP.
Journal: Tsitologiia (1982): 1233
Authors: Phaire-Washington L, Silverstein SC, Wang E.
Journal: J Cell Biol (1980): 641
Authors: Einck KH, Norkin LC.
Journal: Intervirology (1979): 47