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Cell Navigator® NBD Ceramide Golgi Staining Kit
Green Fluorescence
The Golgi apparatus is a complex of vesicles and folded membranes within the cytoplasm of most eukaryotic cells, involved in secretion and intracellular transport. It modifies proteins and lipids that have been built in the endoplasmic reticulum (ER) and prepares them for export outside of the cell. It also plays a significant role in the transport of lipids throughout the cell and the formation of lysosomes. This Cell Navigator® NBD Ceramide Golgi Staining kit provides a simple and rapid way to stain Golgi in live cells, or aldehyde-fixed cells selectively. C6 NBD Ceramide is administered to cells as a complex with bovine serum albumin (C6-NBD-Ceramide-BSA). Golgi apparatus is stained through the formation of the respective fluorescent metabolites. This Cell Navigator® NBD Ceramide Golgi Staining Kit provides an optimized assay method for examining the morphology of the Golgi apparatus with a fluorescence microscope.
The fluorescence image of NBD Ceramide Golgi Staining in HeLa cells. Cells were stained with 100 µL of C6 NBD Ceramide working solution at 37 °C for 20min and followed  with Hoechst 33342 stain. An intensely fluorescent threadlike structure, partially surround the nucleus, is identified as the Golgi apparatus.
The fluorescence image of NBD Ceramide Golgi Staining in HeLa cells. Cells were stained with 100 µL of C6 NBD Ceramide working solution at 37 °C for 20min and followed  with Hoechst 33342 stain. An intensely fluorescent threadlike structure, partially surround the nucleus, is identified as the Golgi apparatus.
protocol
Protocol
sds
SDS
COO
COA
CatalogSize
Price
Quantity
22750100 Tests
Price
 
Spectral properties
Excitation (nm)467
Emission (nm)538
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Fluorescence microscope
Excitation488 nm
Emission525 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)FITC filterset
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Page updated on October 7, 2025