Cell Navigator® TMR Ceramide Golgi Staining Kit *Red Fluorescence*

Cell Navigator® TMR Ceramide Golgi Staining Kit

Red Fluorescence

The Golgi apparatus is a complex of vesicles and folded membranes within the cytoplasm of most eukaryotic cells, involved in secretion and intracellular transport. It modifies proteins and lipids that have been built in the endoplasmic reticulum (ER) and prepares them for export outside of the cell. It also plays a significant role in the transport of lipids throughout the cell and the formation of lysosomes. Cell Navigator® TMR Ceramide Golgi Staining kit provides a simple and rapid way to stain Golgi in red fluorescence in live cells. Golgi apparatus is stained through the formation of the respective fluorescent metabolites. Cell Navigator® TMR Ceramide Golgi Staining Kit provides an optimized assay method for examining the morphology of the Golgi apparatus with a fluorescence microscope. In addition, GGR169-ceramide is pH-sensitive, thus uniquely serving as an excellent Golgi pH probe too.

<strong>The representative fluorescence image of GGR169 Ceramide Golgi Staining in HeLa cells.</strong> Cells were stained with 100 µL of GGR169-ceramide working solution with Hoechst 33342 at 37 °C for 20 minutes. An intensely fluorescent thread like structure, partially surround the nucleus, is identified as the Golgi apparatus.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22752	100 Tests	Price

References

View all 50 references: Citation Explorer

The role of ceramide in regulating endoplasmic reticulum function.

Authors:

Zelnik, Iris D and Ventura, Ana E and Kim, Jiyoon L and Silva, Liana C and Futerman, Anthony H

Journal:

Biochimica et biophysica acta. Molecular and cell biology of lipids (2020): 158489

Chlamydia trachomatis-infected human cells convert ceramide to sphingomyelin without sphingomyelin synthases 1 and 2.

Authors:

Tachida, Yuriko and Kumagai, Keigo and Sakai, Shota and Ando, Shuji and Yamaji, Toshiyuki and Hanada, Kentaro

Journal:

FEBS letters (2020): 519-529

Structure, functions and regulation of CERT, a lipid-transfer protein for the delivery of ceramide at the ER-Golgi membrane contact sites.

Authors:

Kumagai, Keigo and Hanada, Kentaro

Journal:

FEBS letters (2019): 2366-2377

Neuronal ceroid lipofuscinosis related ER membrane protein CLN8 regulates PP2A activity and ceramide levels.

Authors:

Adhikari, Babita and De Silva, Bhagya and Molina, Joshua A and Allen, Ashton and Peck, Sun H and Lee, Stella Y

Journal:

Biochimica et biophysica acta. Molecular basis of disease (2019): 322-328

Both the N- and C- terminal regions of the Chlamydial inclusion protein D (IncD) are required for interaction with the pleckstrin homology domain of the ceramide transport protein CERT.

Authors:

Kumagai, Keigo and Elwell, Cherilyn A and Ando, Shuji and Engel, Joanne N and Hanada, Kentaro

Journal:

Biochemical and biophysical research communications (2018): 1070-1076

Physical properties

Solvent

DMSO

Spectral properties

Excitation (nm)	544
Emission (nm)	570

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	Cy3/TRITC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom

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Page updated on September 30, 2025

Component A: GGR169-ceramide	1 vial
Component B: Staining Buffer	1 bottle (25 mL)
Component C: DMSO	1 vial (200 µL)
Component D: Hoechst 33342	1 vial (50 µL)