Name: CellPaint™ TSP membrane stain
Brand: AAT Bioquest
SKU: 22700
Price: 232 USD
Availability: InStock

CellPaint™ TSP membrane stain

TSP is a styrylpyridine-based fluorescent membrane probe suitable for imaging plasma membranes in living cells and tissues. It was reported by Guo et al in 2016 (Analyst, 2016, 141, 3228). The probe is a molecular rotor that has fluorescence sharply enhanced in viscous media. Its fluorescence is also microenvironment-sensitive, enables the turn-on imaging of plasma membranes with a high signal-to-noise ratio. Guo et al has demonstrated that TSP has high photostability, low cytotoxicity and excellent biocompatibility. It can also be used in 2 photon imaging.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22700	500 Tests	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CellPaint™ TSP membrane stain to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	98.399 µL	491.995 µL	983.99 µL	4.92 mL	9.84 mL
5 mM	19.68 µL	98.399 µL	196.798 µL	983.99 µL	1.968 mL
10 mM	9.84 µL	49.2 µL	98.399 µL	491.995 µL	983.99 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1016.27 g/mol	=	mL	x	mM	=	mmol

Citations

View all 31 citations: Citation Explorer

Membrane trafficking and exocytosis are upregulated in port wine stain blood vessels

Authors:

Yin, R., Rice, S. J., Wang, J., Gao, L., Tsai, J., Anvari, R. T., Zhou, F., Liu, X., Wang, G., Tang, Y., Mihm, M. C., Jr., Belani, C. P., Chen, D. B., Nelson, J. S., Tan, W.

Journal:

Histol Histopathol (2019): 479-490

Cu(2+)-Directed Liposome Membrane Fusion, Positive-Stain Electron Microscopy, and Oxidation

Authors:

Liu, Y., Liu, J.

Journal:

Langmuir (2018): 7545-7553

Extraction of DNA from Sperm Cells in Mixed Stain by Nylon Membrane Bushing Separation Technique

Authors:

Ma, J., Tong, Q., Gao, L. B., Zhu, C., Jiang, Z. Q.

Journal:

Fa Yi Xue Za Zhi (2018): 417-419

Gram's Stain Does Not Cross the Bacterial Cytoplasmic Membrane

Authors:

Wilhelm, M. J., Sheffield, J. B., Sharifian Gh, M., Wu, Y., Spahr, C., Gonella, G., Xu, B., Dai, H. L.

Journal:

ACS Chem Biol (2015): 1711-7

The use of SMALPs as a novel membrane protein scaffold for structure study by negative stain electron microscopy

Authors:

Postis, V., Rawson, S., Mitchell, J. K., Lee, S. C., Parslow, R. A., Dafforn, T. R., Baldwin, S. A., Muench, S. P.

Journal:

Biochim Biophys Acta (2015): 496-501

Physical properties

Molecular weight	1016.27
Solvent	DMSO

Spectral properties

Excitation (nm)	496
Emission (nm)	633

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	Cy3/TRTC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom

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Page updated on October 13, 2025