CF-MU [6-Chloro-8-fluoro-umbelliferone] *Fluorescence reference standard*
Ordering information
Price | |
Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 228.6 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 356 |
Emission (nm) | 456 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Coumarins
Molecular weight 228.6 | Excitation (nm) 356 | Emission (nm) 456 |
This compound is used as a calibration standard for CF-MU-based enzyme substrates. It has pH-dependent fluorescence. Compared to umbelliferone, this dye has much lower pKa, making it more fluorescent than umbelliferone under physiological conditions.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of CF-MU [6-Chloro-8-fluoro-umbelliferone] *Fluorescence reference standard* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 437.445 µL | 2.187 mL | 4.374 mL | 21.872 mL | 43.745 mL |
5 mM | 87.489 µL | 437.445 µL | 874.891 µL | 4.374 mL | 8.749 mL |
10 mM | 43.745 µL | 218.723 µL | 437.445 µL | 2.187 mL | 4.374 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
References
View all 10 references: Citation Explorer
A Novel Fluorogenic Coumarin Substrate for Monitoring Acid Phosphatase Activity at Low pH Environment
Authors: Yang D, Z Li, Y Diwu, H Fu, J Liao, C Wei, and Z Diwu, undefined
Journal: Curr Chem Genomics (2008): 48–50
Authors: Yang D, Z Li, Y Diwu, H Fu, J Liao, C Wei, and Z Diwu, undefined
Journal: Curr Chem Genomics (2008): 48–50
Excited state properties of 7-hydroxy-4-methylcoumarin in the gas phase and in solution. A theoretical study
Authors: Georgieva I, Trendafilova N, Aquino A, Lischka H.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 11860
Authors: Georgieva I, Trendafilova N, Aquino A, Lischka H.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 11860
Novel thiocoumarins as inhibitors of TNF-alpha induced ICAM-1 expression on human umbilical vein endothelial cells (HUVECs) and microsomal lipid peroxidation
Authors: Kumar S, Singh BK, Kalra N, Kumar V, Kumar A, Prasad AK, Raj HG, Parmar VS, Ghosh B.
Journal: Bioorg Med Chem (2005): 1605
Authors: Kumar S, Singh BK, Kalra N, Kumar V, Kumar A, Prasad AK, Raj HG, Parmar VS, Ghosh B.
Journal: Bioorg Med Chem (2005): 1605
Stability of the complexes of some lanthanides with coumarin derivatives. I. Cerium(III)-4-methyl-7-hydroxycoumarin
Authors: Kostova IP, Manolov, II, Radulova MK.
Journal: Acta Pharm (2004): 37
Authors: Kostova IP, Manolov, II, Radulova MK.
Journal: Acta Pharm (2004): 37
Measurement of enzyme kinetics using microscale steady-state kinetic analysis
Authors: Gleason NJ, Carbeck JD.
Journal: Langmuir (2004): 6374
Authors: Gleason NJ, Carbeck JD.
Journal: Langmuir (2004): 6374
Structural requirements of hydroxylated coumarins for in vitro anti-Helicobacter pylori activity
Authors: Kawase M, Tanaka T, Sohara Y, Tani S, Sakagami H, Hauer H, Chatterjee SS.
Journal: In Vivo (2003): 509
Authors: Kawase M, Tanaka T, Sohara Y, Tani S, Sakagami H, Hauer H, Chatterjee SS.
Journal: In Vivo (2003): 509
Crystal structure of 7,8-dihydroxy-4-methylcoumarin
Authors: Kurosaki H, Sharma RK, Otsuka M, Goto M.
Journal: Anal Sci (2003): 647
Authors: Kurosaki H, Sharma RK, Otsuka M, Goto M.
Journal: Anal Sci (2003): 647
Modifying rates of reductive elimination of leaving groups from indolequinone prodrugs: a key factor in controlling hypoxia-selective drug release
Authors: Everett SA, Swann E, Naylor MA, Stratford MR, Patel KB, Tian N, Newman RG, Vojnovic B, Moody CJ, Wardman P.
Journal: Biochem Pharmacol (2002): 1629
Authors: Everett SA, Swann E, Naylor MA, Stratford MR, Patel KB, Tian N, Newman RG, Vojnovic B, Moody CJ, Wardman P.
Journal: Biochem Pharmacol (2002): 1629
Effects of 18 alpha-glycyrrhizic acid on rat liver cytochrome P450 isoenzymes and phase II transferase
Authors: Yang J, Peng RX, Kong R, Yu JP.
Journal: Yao Xue Xue Bao (2001): 321
Authors: Yang J, Peng RX, Kong R, Yu JP.
Journal: Yao Xue Xue Bao (2001): 321
Synthesis and evaluation of novel fluorogenic substrates for the detection of bacterial beta-galactosidase
Authors: Chilvers KF, Perry JD, James AL, Reed RH.
Journal: J Appl Microbiol (2001): 1118
Authors: Chilvers KF, Perry JD, James AL, Reed RH.
Journal: J Appl Microbiol (2001): 1118
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