Name: CF-MU [6-Chloro-8-fluoro-umbelliferone] *Fluorescence reference standard*
Brand: AAT Bioquest
SKU: 42
Price: 115 USD
Availability: InStock

CF-MU

6-Chloro-8-fluoro-umbelliferone; Fluorescence reference standard

This compound is used as a calibration standard for CF-MU-based enzyme substrates. It has pH-dependent fluorescence. Compared to umbelliferone, this dye has much lower pKa, making it more fluorescent than umbelliferone under physiological conditions.

Protocol

SDS

Catalog	Size	Price	Quantity
42	100 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CF-MU [6-Chloro-8-fluoro-umbelliferone] *Fluorescence reference standard* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	437.445 µL	2.187 mL	4.374 mL	21.872 mL	43.745 mL
5 mM	87.489 µL	437.445 µL	874.891 µL	4.374 mL	8.749 mL
10 mM	43.745 µL	218.723 µL	437.445 µL	2.187 mL	4.374 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	228.6 g/mol	=	mL	x	mM	=	mmol

References

View all 10 references: Citation Explorer

A Novel Fluorogenic Coumarin Substrate for Monitoring Acid Phosphatase Activity at Low pH Environment

Authors:

Yang D, Z Li, Y Diwu, H Fu, J Liao, C Wei, and Z Diwu, undefined

Journal:

Curr Chem Genomics (2008): 48–50

Novel thiocoumarins as inhibitors of TNF-alpha induced ICAM-1 expression on human umbilical vein endothelial cells (HUVECs) and microsomal lipid peroxidation

Authors:

Kumar S, Singh BK, Kalra N, Kumar V, Kumar A, Prasad AK, Raj HG, Parmar VS, Ghosh B.

Journal:

Bioorg Med Chem (2005): 1605

Excited state properties of 7-hydroxy-4-methylcoumarin in the gas phase and in solution. A theoretical study

Authors:

Georgieva I, Trendafilova N, Aquino A, Lischka H.

Journal:

J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 11860

Measurement of enzyme kinetics using microscale steady-state kinetic analysis

Authors:

Gleason NJ, Carbeck JD.

Journal:

Langmuir (2004): 6374

Stability of the complexes of some lanthanides with coumarin derivatives. I. Cerium(III)-4-methyl-7-hydroxycoumarin

Authors:

Kostova IP, Manolov, II, Radulova MK.

Journal:

Acta Pharm (2004): 37

Physical properties

Molecular weight	228.6
Solvent	DMSO

Spectral properties

Excitation (nm)	356
Emission (nm)	456

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Enzyme Probes & Assay Kits

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Page updated on November 5, 2025