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CF-MU [6-Chloro-8-fluoro-umbelliferone] *Fluorescence reference standard*

This compound is used as a calibration standard for CF-MU-based enzyme substrates. It has pH-dependent fluorescence. Compared to umbelliferone, this dye has much lower pKa, making it more fluorescent than umbelliferone under physiological conditions.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CF-MU [6-Chloro-8-fluoro-umbelliferone] *Fluorescence reference standard* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM437.445 µL2.187 mL4.374 mL21.872 mL43.745 mL
5 mM87.489 µL437.445 µL874.891 µL4.374 mL8.749 mL
10 mM43.745 µL218.723 µL437.445 µL2.187 mL4.374 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

References

View all 10 references: Citation Explorer
A Novel Fluorogenic Coumarin Substrate for Monitoring Acid Phosphatase Activity at Low pH Environment
Authors: Yang D, Z Li, Y Diwu, H Fu, J Liao, C Wei, and Z Diwu, undefined
Journal: Curr Chem Genomics (2008): 48–50
Novel thiocoumarins as inhibitors of TNF-alpha induced ICAM-1 expression on human umbilical vein endothelial cells (HUVECs) and microsomal lipid peroxidation
Authors: Kumar S, Singh BK, Kalra N, Kumar V, Kumar A, Prasad AK, Raj HG, Parmar VS, Ghosh B.
Journal: Bioorg Med Chem (2005): 1605
Excited state properties of 7-hydroxy-4-methylcoumarin in the gas phase and in solution. A theoretical study
Authors: Georgieva I, Trendafilova N, Aquino A, Lischka H.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 11860
Measurement of enzyme kinetics using microscale steady-state kinetic analysis
Authors: Gleason NJ, Carbeck JD.
Journal: Langmuir (2004): 6374
Stability of the complexes of some lanthanides with coumarin derivatives. I. Cerium(III)-4-methyl-7-hydroxycoumarin
Authors: Kostova IP, Manolov, II, Radulova MK.
Journal: Acta Pharm (2004): 37
Page updated on December 11, 2024

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Physical properties

Molecular weight

228.6

Solvent

DMSO

Spectral properties

Excitation (nm)

356

Emission (nm)

456

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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