CF-MUP, sodium salt *Superior alternative to MUP*

CF-MUP, sodium salt

Superior alternative to MUP

Although MUP is widely used for detecting phosphatases in solution it is not well suited for living cell or continuous assays since MU (4-methylumbelliferone), the enzymatic product, which only develops maximum fluorescence at pH value of >10. Thus it is also difficult to use MUP for the detection of phosphatases that have acidic optimal pH range such as acid phosphatases. AAT Bioquest is pleased to offer CF-MUP Plus that is developed to address this pH limitation associated with MUP substrates. CF-MU exhibits maximum fluorescence above pH 5.0, thus CF-MUP substrate can be well used for continuous phosphatase assays. It can also be used for the assays that require acidic pH such as acid phosphatases and some protein phosphatases.

Detection of acid phosphatase activity CF-MUP and MUP. The concentration of the two substrates (initially approximately 10 uM) were matched by normalizing the obsorbance at 319 nm (pH = 10) to a value of 0.52 (assuming the extinction coefficient of each substrate was approximately equivalent). The resulting fluorescence signal was recorded using Ex/Em = 360/450 nm.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11628	10 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of CF-MUP, sodium salt *Superior alternative to MUP* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	283.648 µL	1.418 mL	2.836 mL	14.182 mL	28.365 mL
5 mM	56.73 µL	283.648 µL	567.295 µL	2.836 mL	5.673 mL
10 mM	28.365 µL	141.824 µL	283.648 µL	1.418 mL	2.836 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	352.55 g/mol	=	mL	x	mM	=	mmol

References

View all 76 references: Citation Explorer

Application of intracellular alkaline phosphatase activity measurement in detection of neutrophil adherence in vitro

Authors:

Bednarska K, Klink M, Sulowska Z.

Journal:

Mediators Inflamm (2006): 19307

Evaluation of an alternative method for the enumeration and confirmation of Clostridium perfringens from treated and untreated sewages

Authors:

Wohlsen T, Bayliss J, Gray B, Bates J, Katouli M.

Journal:

Lett Appl Microbiol (2006): 438

Fluorometric cell-ELISA for quantifying rabies infection and heparin inhibition

Authors:

Rincon V, Corredor A, Martinez-Gutierrez M, Castellanos JE.

Journal:

J Virol Methods (2005): 33

Relative movement and soil fixation of soluble organic and inorganic phosphorus

Authors:

Anderson BH, Magdoff FR.

Journal:

J Environ Qual (2005): 2228

Purification and partial characterization of an acid phosphatase from Spirodela oligorrhiza and its affinity for selected organophosphate pesticides

Authors:

Hoehamer CF, Mazur CS, Wolfe NL.

Journal:

J Agric Food Chem (2005): 90

Physical properties

Molecular weight	352.55
Solvent	Water

Spectral properties

Excitation (nm)	356
Emission (nm)	456

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on July 12, 2023