Chemical Phosphorylation Reagent I (CPR I)

This reagent can be used to create the 3'-phosphate by adding it as the first addition to the support. After ammonia deprotection the oligo will have the 3'-phosphate attached to the 2nd base added during synthesis. Both the support base and the CPR are cleaved. The DMT-group is removed during the ammonium hydroxide deprotection and thus is not available for poly-pak purification.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
6001	1 g	Price

Calculators

Common stock solution preparation

Table 1. Volume of MeCN needed to reconstitute specific mass of Chemical Phosphorylation Reagent I (CPR I) to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	152.26 µL	761.302 µL	1.523 mL	7.613 mL	15.226 mL
5 mM	30.452 µL	152.26 µL	304.521 µL	1.523 mL	3.045 mL
10 mM	15.226 µL	76.13 µL	152.26 µL	761.302 µL	1.523 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	656.77 g/mol	=	mL	x	mM	=	mmol

References

View all 9 references: Citation Explorer

Chemical synthesis and molecular recognition of phosphatase-resistant analogues of phosphatidylinositol-3-phosphate

Authors:

Xu Y, Lee SA, Kutateladze TG, Sbrissa D, Shisheva A, Prestwich GD.

Journal:

J Am Chem Soc (2006): 885

O-selectivity and utility of phosphorylation mediated by phosphite triester intermediates in the N-unprotected phosphoramidite method

Authors:

Ohkubo A, Ezawa Y, Seio K, Sekine M.

Journal:

J Am Chem Soc (2004): 10884

Synthesis and conformational properties of oligonucleotides incorporating 2'-O-phosphorylated ribonucleotides as structural motifs of pre-tRNA splicing intermediates

Authors:

Tsuruoka H, Shohda K, Wada T, Sekine M.

Journal:

J Org Chem (2000): 7479

Photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides

Authors:

Olejnik J, Krzymanska-Olejnik E, Rothschild KJ.

Journal:

Nucleic Acids Res (1996): 361

A new approach to phosphoserine, phosphothreonine and phosphotyrosine synthons and to thiophospho analogs. Stepwise synthesis of mono- and multiphosphorylated phosphopeptides related to src-protein kinase

Authors:

Mora N, Lacombe JM, Pavia AA.

Journal:

Int J Pept Protein Res (1995): 53

Physical properties

Molecular weight	656.77
Solvent	MeCN

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 13, 2025