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Chemical Phosphorylation Reagent I (CPR I)
This reagent can be used to create the 3'-phosphate by adding it as the first addition to the support. After ammonia deprotection the oligo will have the 3'-phosphate attached to the 2nd base added during synthesis. Both the support base and the CPR are cleaved. The DMT-group is removed during the ammonium hydroxide deprotection and thus is not available for poly-pak purification.
Calculators
Common stock solution preparation
Table 1. Volume of MeCN needed to reconstitute specific mass of Chemical Phosphorylation Reagent I (CPR I) to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 152.26 µL | 761.302 µL | 1.523 mL | 7.613 mL | 15.226 mL |
| 5 mM | 30.452 µL | 152.26 µL | 304.521 µL | 1.523 mL | 3.045 mL |
| 10 mM | 15.226 µL | 76.13 µL | 152.26 µL | 761.302 µL | 1.523 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
References
View all 9 references: Citation Explorer
Chemical synthesis and molecular recognition of phosphatase-resistant analogues of phosphatidylinositol-3-phosphate
Authors: Xu Y, Lee SA, Kutateladze TG, Sbrissa D, Shisheva A, Prestwich GD.
Journal: J Am Chem Soc (2006): 885
Authors: Xu Y, Lee SA, Kutateladze TG, Sbrissa D, Shisheva A, Prestwich GD.
Journal: J Am Chem Soc (2006): 885
O-selectivity and utility of phosphorylation mediated by phosphite triester intermediates in the N-unprotected phosphoramidite method
Authors: Ohkubo A, Ezawa Y, Seio K, Sekine M.
Journal: J Am Chem Soc (2004): 10884
Authors: Ohkubo A, Ezawa Y, Seio K, Sekine M.
Journal: J Am Chem Soc (2004): 10884
Synthesis and conformational properties of oligonucleotides incorporating 2'-O-phosphorylated ribonucleotides as structural motifs of pre-tRNA splicing intermediates
Authors: Tsuruoka H, Shohda K, Wada T, Sekine M.
Journal: J Org Chem (2000): 7479
Authors: Tsuruoka H, Shohda K, Wada T, Sekine M.
Journal: J Org Chem (2000): 7479
Photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides
Authors: Olejnik J, Krzymanska-Olejnik E, Rothschild KJ.
Journal: Nucleic Acids Res (1996): 361
Authors: Olejnik J, Krzymanska-Olejnik E, Rothschild KJ.
Journal: Nucleic Acids Res (1996): 361
A new approach to phosphoserine, phosphothreonine and phosphotyrosine synthons and to thiophospho analogs. Stepwise synthesis of mono- and multiphosphorylated phosphopeptides related to src-protein kinase
Authors: Mora N, Lacombe JM, Pavia AA.
Journal: Int J Pept Protein Res (1995): 53
Authors: Mora N, Lacombe JM, Pavia AA.
Journal: Int J Pept Protein Res (1995): 53
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