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Chemical Phosphorylation Reagent I (CPR I)

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Physical properties
Molecular weight656.77
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Molecular weight
This reagent can be used to create the 3'-phosphate by adding it as the first addition to the support. After ammonia deprotection the oligo will have the 3'-phosphate attached to the 2nd base added during synthesis. Both the support base and the CPR are cleaved. The DMT-group is removed during the ammonium hydroxide deprotection and thus is not available for poly-pak purification.


Common stock solution preparation

Table 1. Volume of MeCN needed to reconstitute specific mass of Chemical Phosphorylation Reagent I (CPR I) to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM152.26 µL761.302 µL1.523 mL7.613 mL15.226 mL
5 mM30.452 µL152.26 µL304.521 µL1.523 mL3.045 mL
10 mM15.226 µL76.13 µL152.26 µL761.302 µL1.523 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles



View all 9 references: Citation Explorer
Chemical synthesis and molecular recognition of phosphatase-resistant analogues of phosphatidylinositol-3-phosphate
Authors: Xu Y, Lee SA, Kutateladze TG, Sbrissa D, Shisheva A, Prestwich GD.
Journal: J Am Chem Soc (2006): 885
O-selectivity and utility of phosphorylation mediated by phosphite triester intermediates in the N-unprotected phosphoramidite method
Authors: Ohkubo A, Ezawa Y, Seio K, Sekine M.
Journal: J Am Chem Soc (2004): 10884
Synthesis and conformational properties of oligonucleotides incorporating 2'-O-phosphorylated ribonucleotides as structural motifs of pre-tRNA splicing intermediates
Authors: Tsuruoka H, Shohda K, Wada T, Sekine M.
Journal: J Org Chem (2000): 7479
Photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides
Authors: Olejnik J, Krzymanska-Olejnik E, Rothschild KJ.
Journal: Nucleic Acids Res (1996): 361
A new approach to phosphoserine, phosphothreonine and phosphotyrosine synthons and to thiophospho analogs. Stepwise synthesis of mono- and multiphosphorylated phosphopeptides related to src-protein kinase
Authors: Mora N, Lacombe JM, Pavia AA.
Journal: Int J Pept Protein Res (1995): 53
Synthesis of sphingosine-1-phosphate and dihydrosphingosine-1-phosphate
Authors: Boumendjel A, Miller SP.
Journal: J Lipid Res (1994): 2305
Chemical synthesis of O-thiophosphotyrosyl peptides
Authors: Kitas E, Kung E, Bannwarth W.
Journal: Int J Pept Protein Res (1994): 146
Syntheses of D-myo-inositol 1,4,5-trisphosphate affinity ligands
Authors: Tegge W, Ballou CE.
Journal: Carbohydr Res (1992): 63
Fmoc/solid-phase synthesis of Tyr(P)-containing peptides through t-butyl phosphate protection
Authors: Perich JW, Reynolds EC.
Journal: Int J Pept Protein Res (1991): 572