Name: Cl-NERF
Brand: AAT Bioquest
SKU: 21220
Price: 232 USD
Availability: InStock

Cl-NERF

Cl-NERF is a rhodol dye with pKa = 3.8. It has been mainly used for monitoring pH in acidic environments such as endosomes and lysosomes. Cl-NERF has a ratiometric excitation spectrum with 510/450 nm excitation ratio commonly used. The dextran conjugates of Cl-NERF has been used for detect the uptake of labeled probes into acidic organelles.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21220	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cl-NERF to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	221.307 µL	1.107 mL	2.213 mL	11.065 mL	22.131 mL
5 mM	44.261 µL	221.307 µL	442.615 µL	2.213 mL	4.426 mL
10 mM	22.131 µL	110.654 µL	221.307 µL	1.107 mL	2.213 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	451.86 g/mol	=	mL	x	mM	=	mmol

References

View all 12 references: Citation Explorer

Inhibitors of acid secretion can benefit gastric wound repair independent of luminal pH effects on the site of damage

Authors:

Demitrack ES, Aihara E, Kenny S, Varro A, Montrose MH.

Journal:

Gut (2012): 804

Damage to the gastric epithelium activates cellular bicarbonate secretion via SLC26A9 Cl(-)/HCO(3)(-)

Authors:

Demitrack ES, Soleimani M, Montrose MH.

Journal:

Am J Physiol Gastrointest Liver Physiol (2010): G255

In vivo action of trefoil factor 2 (TFF2) to speed gastric repair is independent of cyclooxygenase

Authors:

Xue L, Aihara E, Podolsky DK, Wang TC, Montrose MH.

Journal:

Gut (2010): 1184

Disruption of the Cox-1 gene slows repair of microscopic lesions in the mouse gastric epithelium

Authors:

Starodub OT, Demitrack ES, Baumgartner HK, Montrose MH.

Journal:

Am J Physiol Cell Physiol (2008): C223

Cyclooxygenase 1 is required for pH control at the mouse gastric surface

Authors:

Baumgartner HK, Starodub OT, Joehl JS, Tackett L, Montrose MH.

Journal:

Gut (2004): 1751

Physical properties

Molecular weight	451.86
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 12, 2025