CLF [Cholyl-Lys-Fluorescein]

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Physical properties

Molecular weight	926.12
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CLF [Cholyl-Lys-Fluorescein] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	107.977 µL	539.887 µL	1.08 mL	5.399 mL	10.798 mL
5 mM	21.595 µL	107.977 µL	215.955 µL	1.08 mL	2.16 mL
10 mM	10.798 µL	53.989 µL	107.977 µL	539.887 µL	1.08 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Images

Figure 1. Chemical structure for CLF [Cholyl-Lys-Fluorescein]

Citations

View all 1 citations: Citation Explorer

A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis
Authors: Kang, Soo-Yeon and Kimura, Masaki and Shrestha, Sunil and Lewis, Phillip and Lee, Sangjoon and Cai, Yuqi and Joshi, Pranav and Acharya, Prabha and Liu, Jiafeng and Yang, Yong and others,
Journal: bioRxiv (2023): 2023--03

References

View all 42 references: Citation Explorer

Inhibition of bile acid transport across Na+/taurocholate cotransporting polypeptide (SLC10A1) and bile salt export pump (ABCB 11)-coexpressing LLC-PK1 cells by cholestasis-inducing drugs
Authors: Mita S, Suzuki H, Akita H, Hayashi H, Onuki R, Hofmann AF, Sugiyama Y.
Journal: Drug Metab Dispos (2006): 1575

Oxidative stress induces internalization of the bile salt export pump, Bsep, and bile salt secretory failure in isolated rat hepatocyte couplets: a role for protein kinase C and prevention by protein kinase A
Authors: Perez LM, Milkiewicz P, Elias E, Coleman R, Sanchez Pozzi EJ, Roma MG.
Journal: Toxicol Sci (2006): 150

Radixin is required to maintain apical canalicular membrane structure and function in rat hepatocytes
Authors: Wang W, Soroka CJ, Mennone A, Rahner C, Harry K, Pypaert M, Boyer JL.
Journal: Gastroenterology (2006): 878

Small hepatocytes in culture develop polarized transporter expression and differentiation
Authors: Sidler Pf, undefined and ler MA, Hochli M, Inderbitzin D, Meier PJ, Stieger B.
Journal: J Cell Sci (2004): 4077

Vectorial transport of bile acids in immortalized mouse bile duct cells
Authors: Kida M, Mano Y, Ueno Y, Kobayashi K, Goto J, Ishii M, Shimosegawa T.
Journal: Hepatol Res (2003): 151

Cyclosporin A induced internalization of the bile salt export pump in isolated rat hepatocyte couplets
Authors: Roman ID, Fern and ez-Moreno MD, Fueyo JA, Roma MG, Coleman R.
Journal: Toxicol Sci (2003): 276

Taurolithocholic acid exerts cholestatic effects via phosphatidylinositol 3-kinase-dependent mechanisms in perfused rat livers and rat hepatocyte couplets
Authors: Beuers U, Denk GU, Soroka CJ, Wimmer R, Rust C, Paumgartner G, Boyer JL.
Journal: J Biol Chem (2003): 17810

Hepatoprotection with tauroursodeoxycholate and beta muricholate against taurolithocholate induced cholestasis: involvement of signal transduction pathways
Authors: Milkiewicz P, Roma MG, Elias E, Coleman R.
Journal: Gut (2002): 113

Visualization of the transport of primary and secondary bile acids across liver tissue in rats: in vivo study with fluorescent bile acids
Authors: Milkiewicz P, Mills CO, Hubscher SG, Cardenas R, Cardenas T, Williams A, Elias E.
Journal: J Hepatol (2001): 4

Use of a fluorescent bile acid to enhance visualization of the biliary tract and bile leaks during laparoscopic surgery in rabbits
Authors: Holzinger F, Krahenbuhl L, Schteingart CD, Ton-Nu HT, Hofmann AF.
Journal: Surg Endosc (2001): 209