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CLF [Cholyl-Lys-Fluorescein]
CLF is a fluorescein-labeled bile acid analog that closely parallels the cellular binding and uptake properties of cholic acid derivatives. It is a divalent 'unipolar' anionic fluorescent monohydroxy bile salt analog with physical, biological and cholestatic properties that are similar to those of lithocholate, glycolithocholate and their derivatives and thus offers a potentially useful probe for studying mechanisms of monohydroxy bile salt-induced cholestasis at the hepatocellular level. CLF is currently used as a tool for the visualization of bile acid transport within liver tissue.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of CLF [Cholyl-Lys-Fluorescein] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 107.977 µL | 539.887 µL | 1.08 mL | 5.399 mL | 10.798 mL |
| 5 mM | 21.595 µL | 107.977 µL | 215.955 µL | 1.08 mL | 2.16 mL |
| 10 mM | 10.798 µL | 53.989 µL | 107.977 µL | 539.887 µL | 1.08 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Citations
View all 2 citations: Citation Explorer
Reproducible generation of human liver organoids (HLOs) on a pillar plate platform via microarray 3D bioprinting
Authors: Shrestha, Sunil and Lekkala, Vinod Kumar Reddy and Acharya, Prabha and Kang, Soo-Yeon and Vanga, Manav Goud and Lee, Moo-Yeal
Journal: bioRxiv (2024): 2024--03
Authors: Shrestha, Sunil and Lekkala, Vinod Kumar Reddy and Acharya, Prabha and Kang, Soo-Yeon and Vanga, Manav Goud and Lee, Moo-Yeal
Journal: bioRxiv (2024): 2024--03
A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis
Authors: Kang, Soo-Yeon and Kimura, Masaki and Shrestha, Sunil and Lewis, Phillip and Lee, Sangjoon and Cai, Yuqi and Joshi, Pranav and Acharya, Prabha and Liu, Jiafeng and Yang, Yong and others,
Journal: bioRxiv (2023): 2023--03
Authors: Kang, Soo-Yeon and Kimura, Masaki and Shrestha, Sunil and Lewis, Phillip and Lee, Sangjoon and Cai, Yuqi and Joshi, Pranav and Acharya, Prabha and Liu, Jiafeng and Yang, Yong and others,
Journal: bioRxiv (2023): 2023--03
References
View all 42 references: Citation Explorer
Inhibition of bile acid transport across Na+/taurocholate cotransporting polypeptide (SLC10A1) and bile salt export pump (ABCB 11)-coexpressing LLC-PK1 cells by cholestasis-inducing drugs
Authors: Mita S, Suzuki H, Akita H, Hayashi H, Onuki R, Hofmann AF, Sugiyama Y.
Journal: Drug Metab Dispos (2006): 1575
Authors: Mita S, Suzuki H, Akita H, Hayashi H, Onuki R, Hofmann AF, Sugiyama Y.
Journal: Drug Metab Dispos (2006): 1575
Oxidative stress induces internalization of the bile salt export pump, Bsep, and bile salt secretory failure in isolated rat hepatocyte couplets: a role for protein kinase C and prevention by protein kinase A
Authors: Perez LM, Milkiewicz P, Elias E, Coleman R, Sanchez Pozzi EJ, Roma MG.
Journal: Toxicol Sci (2006): 150
Authors: Perez LM, Milkiewicz P, Elias E, Coleman R, Sanchez Pozzi EJ, Roma MG.
Journal: Toxicol Sci (2006): 150
Radixin is required to maintain apical canalicular membrane structure and function in rat hepatocytes
Authors: Wang W, Soroka CJ, Mennone A, Rahner C, Harry K, Pypaert M, Boyer JL.
Journal: Gastroenterology (2006): 878
Authors: Wang W, Soroka CJ, Mennone A, Rahner C, Harry K, Pypaert M, Boyer JL.
Journal: Gastroenterology (2006): 878
Small hepatocytes in culture develop polarized transporter expression and differentiation
Authors: Sidler Pf, undefined and ler MA, Hochli M, Inderbitzin D, Meier PJ, Stieger B.
Journal: J Cell Sci (2004): 4077
Authors: Sidler Pf, undefined and ler MA, Hochli M, Inderbitzin D, Meier PJ, Stieger B.
Journal: J Cell Sci (2004): 4077
Vectorial transport of bile acids in immortalized mouse bile duct cells
Authors: Kida M, Mano Y, Ueno Y, Kobayashi K, Goto J, Ishii M, Shimosegawa T.
Journal: Hepatol Res (2003): 151
Authors: Kida M, Mano Y, Ueno Y, Kobayashi K, Goto J, Ishii M, Shimosegawa T.
Journal: Hepatol Res (2003): 151
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