Name: CLF [Cholyl-Lys-Fluorescein]
Brand: AAT Bioquest
SKU: 36701
Price: 348 USD
Availability: InStock

CLF

Cholyl-Lys-Fluorescein

CLF is a fluorescein-labeled bile acid analog that closely parallels the cellular binding and uptake properties of cholic acid derivatives. It is a divalent 'unipolar' anionic fluorescent monohydroxy bile salt analog with physical, biological and cholestatic properties that are similar to those of lithocholate, glycolithocholate and their derivatives and thus offers a potentially useful probe for studying mechanisms of monohydroxy bile salt-induced cholestasis at the hepatocellular level. CLF is currently used as a tool for the visualization of bile acid transport within liver tissue.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
36701	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CLF [Cholyl-Lys-Fluorescein] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	107.977 µL	539.887 µL	1.08 mL	5.399 mL	10.798 mL
5 mM	21.595 µL	107.977 µL	215.955 µL	1.08 mL	2.16 mL
10 mM	10.798 µL	53.989 µL	107.977 µL	539.887 µL	1.08 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	926.12 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Reproducible generation of human liver organoids (HLOs) on a pillar plate platform via microarray 3D bioprinting

Authors:

Shrestha, Sunil and Lekkala, Vinod Kumar Reddy and Acharya, Prabha and Kang, Soo-Yeon and Vanga, Manav Goud and Lee, Moo-Yeal

Journal:

bioRxiv (2024): 2024--03

A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis

Authors:

Kang, Soo-Yeon and Kimura, Masaki and Shrestha, Sunil and Lewis, Phillip and Lee, Sangjoon and Cai, Yuqi and Joshi, Pranav and Acharya, Prabha and Liu, Jiafeng and Yang, Yong and others,

Journal:

bioRxiv (2023): 2023--03

References

View all 42 references: Citation Explorer

Inhibition of bile acid transport across Na+/taurocholate cotransporting polypeptide (SLC10A1) and bile salt export pump (ABCB 11)-coexpressing LLC-PK1 cells by cholestasis-inducing drugs

Authors:

Mita S, Suzuki H, Akita H, Hayashi H, Onuki R, Hofmann AF, Sugiyama Y.

Journal:

Drug Metab Dispos (2006): 1575

Oxidative stress induces internalization of the bile salt export pump, Bsep, and bile salt secretory failure in isolated rat hepatocyte couplets: a role for protein kinase C and prevention by protein kinase A

Authors:

Perez LM, Milkiewicz P, Elias E, Coleman R, Sanchez Pozzi EJ, Roma MG.

Journal:

Toxicol Sci (2006): 150

Radixin is required to maintain apical canalicular membrane structure and function in rat hepatocytes

Authors:

Wang W, Soroka CJ, Mennone A, Rahner C, Harry K, Pypaert M, Boyer JL.

Journal:

Gastroenterology (2006): 878

Small hepatocytes in culture develop polarized transporter expression and differentiation

Authors:

Sidler Pf, undefined and ler MA, Hochli M, Inderbitzin D, Meier PJ, Stieger B.

Journal:

J Cell Sci (2004): 4077

Vectorial transport of bile acids in immortalized mouse bile duct cells

Authors:

Kida M, Mano Y, Ueno Y, Kobayashi K, Goto J, Ishii M, Shimosegawa T.

Journal:

Hepatol Res (2003): 151

Physical properties

Molecular weight	926.12
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 13, 2025