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Coelenterazine 400a

Bioluminescence, a special form of chemiluminescence, is a natural phenomenon that emits cold light from the reaction catalyzed by the corresponding luciferase in biological systems. The bioluminescent techniques, such as bioluminescence imaging, BRET, and dual-luciferase reporter assay system, have drawn more and more attention due to their broad application in examining various biological processes in vitro and in vivo. This method has low background interference compared to fluorescence in that bioluminescence does not require any excitation light source. Coelenterazine 400a is a bisdeoxy derivative of coelenterazine that has an emission of ~395 nm following conversion by Renilla luciferase (Rluc). It is used in bioluminescence resonance energy transfer (BRET) protocols. Coelenterazine 400a is commonly paired with class 1 and 3 GFP acceptors, including GFP2 and GFP10. BRET assays are widely used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling.

References

View all 3 references: Citation Explorer
Direct comparison of fluorescence- and bioluminescence-based resonance energy transfer methods for real-time monitoring of thrombin-catalysed proteolytic cleavage.
Authors: Dacres, H and Dumancic, M M and Horne, I and Trowell, S C
Journal: Biosensors & bioelectronics (2009): 1164-70
Direct comparison of bioluminescence-based resonance energy transfer methods for monitoring of proteolytic cleavage.
Authors: Dacres, Helen and Dumancic, Mira M and Horne, Irene and Trowell, Stephen C
Journal: Analytical biochemistry (2009): 194-202
Bioluminescence measurements in mice using a skin window.
Authors: Huang, Qin and Acha, Victor and Yow, Raylon and Schneider, Erik and Sardar, Dhiraj K and Hornsby, Peter J
Journal: Journal of biomedical optics (2007): 054012
Page updated on November 9, 2024

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Catalog Number21167
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Physical properties

Molecular weight

391.47

Solvent

Ethanol

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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