Coelenterazine 400a

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Physical properties

Molecular weight	391.47
Solvent	Ethanol

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

See also: Intracellular Ions

Molecular weight

391.47

Bioluminescence, a special form of chemiluminescence, is a natural phenomenon that emits cold light from the reaction catalyzed by the corresponding luciferase in biological systems. The bioluminescent techniques, such as bioluminescence imaging, BRET, and dual-luciferase reporter assay system, have drawn more and more attention due to their broad application in examining various biological processes in vitro and in vivo. This method has low background interference compared to fluorescence in that bioluminescence does not require any excitation light source. Coelenterazine 400a is a bisdeoxy derivative of coelenterazine that has an emission of ~395 nm following conversion by Renilla luciferase (Rluc). It is used in bioluminescence resonance energy transfer (BRET) protocols. Coelenterazine 400a is commonly paired with class 1 and 3 GFP acceptors, including GFP2 and GFP10. BRET assays are widely used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling.

Calculators

Common stock solution preparation

Table 1. Volume of Ethanol needed to reconstitute specific mass of Coelenterazine 400a to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	255.447 µL	1.277 mL	2.554 mL	12.772 mL	25.545 mL
5 mM	51.089 µL	255.447 µL	510.895 µL	2.554 mL	5.109 mL
10 mM	25.545 µL	127.724 µL	255.447 µL	1.277 mL	2.554 mL

Molarity calculator

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Images

Figure 1. Coelenterazine 400a is a bisdeoxy derivative of coelenterazine that has an emission ~395 nm following conversion by Renilla luciferase (Rluc).

References

View all 3 references: Citation Explorer

Direct comparison of fluorescence- and bioluminescence-based resonance energy transfer methods for real-time monitoring of thrombin-catalysed proteolytic cleavage.
Authors: Dacres, H and Dumancic, M M and Horne, I and Trowell, S C
Journal: Biosensors & bioelectronics (2009): 1164-70

Direct comparison of bioluminescence-based resonance energy transfer methods for monitoring of proteolytic cleavage.
Authors: Dacres, Helen and Dumancic, Mira M and Horne, Irene and Trowell, Stephen C
Journal: Analytical biochemistry (2009): 194-202

Bioluminescence measurements in mice using a skin window.
Authors: Huang, Qin and Acha, Victor and Yow, Raylon and Schneider, Erik and Sardar, Dhiraj K and Hornsby, Peter J
Journal: Journal of biomedical optics (2007): 054012