Coelenterazine cp *UltraPure grade*

Coelenterazine cp

UltraPure grade

Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin's bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 µM to >100 µM. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10-20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.

Protocol

COA

Catalog	Size	Price	Quantity
21151	250 ug	Price
21157	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Ethanol needed to reconstitute specific mass of Coelenterazine cp *UltraPure grade* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	240.685 µL	1.203 mL	2.407 mL	12.034 mL	24.069 mL
5 mM	48.137 µL	240.685 µL	481.371 µL	2.407 mL	4.814 mL
10 mM	24.069 µL	120.343 µL	240.685 µL	1.203 mL	2.407 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	415.48 g/mol	=	mL	x	mM	=	mmol

Citations

View all 7 citations: Citation Explorer

Intracellular Ca2+ is important for flagellin-triggered defense in Arabidopsis and involves inositol polyphosphate signaling

Authors:

Ma, Yi and Zhao, Yichen and Berkowitz, Gerald A

Journal:

Journal of Experimental Botany (2017)

Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells

Authors:

Harikumar, Kaleeckal G and Yan, Yan and Xu, Ting-Hai and Melcher, Karsten and Xu, H Eric and Miller, Laurence J

The CLAVATA signaling pathway mediating stem cell fate in shoot meristems requires Ca2+ as a secondary cytosolic messenger

Authors:

Chou, Hsuan and Zhu, Yingfang and Ma, Yi and Berkowitz, Gerald A

Journal:

The Plant Journal (2016): 494--506

Secondary Messengers Involvement in Plant Signal Transduction

Authors:

Chou, Hsuan

Orchestration of salivary secretion mediated by two different dopamine receptors in the blacklegged tick Ixodes scapularis

Authors:

Kim, Donghun and Simo, Ladislav and Park, Yoonseong

Journal:

Journal of Experimental Biology (2014): 3656--3663

References

View all 150 references: Citation Explorer

In vivo testing of Renilla luciferase substrate analogs in an orthotopic murine model of human glioblastoma

Authors:

Otto-Duessel M, Khankaldyyan V, Gonzalez-Gomez I, Jensen MC, Laug WE, Rosol M.

Journal:

Mol Imaging (2006): 57

Mitochondrial adaptations within chronically ischemic swine myocardium

Authors:

McFalls EO, Sluiter W, Schoonderwoerd K, Manintveld OC, Lamers JM, Bezstarosti K, van Beusekom HM, Sikora J, Ward HB, Merkus D, Duncker DJ.

Journal:

J Mol Cell Cardiol (2006): 980

Blue fluorescent protein from the calcium-sensitive photoprotein aequorin: catalytic properties for the oxidation of coelenterazine as an oxygenase

Authors:

Inouye S, Sasaki S.

Journal:

FEBS Lett (2006): 1977

Self-illuminating quantum dots light the way

Authors:

Frangioni JV., undefined

Journal:

Nat Biotechnol (2006): 326

Transient expression of apoaequorin in zebrafish embryos: extending the ability to image calcium transients during later stages of development

Authors:

Cheung CY, Webb SE, Meng A, Miller AL.

Journal:

Int J Dev Biol (2006): 561

Physical properties

Molecular weight	415.48
Solvent	Ethanol

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	123437-25-2

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Page updated on July 26, 2023