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Covidyte™ IF670
Coronaviruses (CoVs) can infect humans and multiple species of animals, causing a wide spectrum of diseases. In late 2019, a novel coronavirus, termed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was determined as a cause for several cases of respiratory disease (Covid-19). The virus rapidly spread worldwide. As of May 31st, 2022, more than 6.2 million people have died from coronavirus worldwide, and ~530 million cases have been reported. Currently, there are not any specific and effective options available for treating Covid-19. At present the clinical treatment of Covid-19 is mainly symptomatic combined with repurposing of already marketed antiviral drugs such as Remdesivir and antibiotics to treat secondary infections. There is an extremely urgent need for the development of specific antiviral therapeutics and vaccines against SARS-CoV-2. The coronavirus main protease, which plays a pivotal role in viral gene expression and replication through the proteolytic processing of replicase polyproteins, is an attractive target for anti-CoV drug design. The inhibition of viral proteases necessary for proteolytic processing of polyproteins has been a successful strategy in the treatment of human immunodeficiency virus (HIV) and hepatitis C respectively, proving the potential of protease inhibitors for the treatment of viral infections. Similarly, the main protease of SARS-CoV-2 is thought to be essential for viral replication and, therefore, is regarded as promising target for antiviral therapy of Covid-19. Covidyte™ IF670 is a peptide substrate containing 12 amino acid sequence (VNSTLQSGLRKM) that can be cleaved by coronavirus proteases. The dark-FRET peptide contains Tide Quencher™ 5 (TQ5) as a quencher and iFluor® 670 as a fluorescent donor on the N-and C-terminals respectively where the fluorescence of iFluor® 670 is effectively quenched by TQ5 when the peptide is intact. When the peptide is hydrolyzed by coronavirus proteases, the iFluor® 670 fragment generates significantly enhanced fluorescence since its fluorescence is no longer quenched by TQ5. The activity of coronavirus proteases can be effectively monitored by the fluorescence intensity of iFluor® 670. Covidyte™ IF670 is a robust high throughput screening tool for searching inhibitors of coronavirus proteases. Comparing to the commonly used EDANS substrates (such as Covidyte™ ED450), the iFluor® 670 substrate has much stronger and longer fluorescence that is less interfered by colored compounds that often cause false positive hits.
Proteases play essential roles in protein activation, cell regulation and signaling, as well as in the generation of amino acids for protein synthesis or utilization in other metabolic pathways. FRET protease substrates are widely used for detecting protease activities, in particular, for virus protease that often require a long peptide sequence for optimal binding such as coronavirus, HIV and HCV proteases. The internally quenched FRET peptide substrate is digested by a protease to generate the highly fluorescent peptide fragment. The fluorescence increase is proportional to the protease activity. Tide Quencher™ dyes have been proven to be the extremely effective quenchers for developing FRET protease substrates for high throughput screening applications together with our bright Tide Fluor™ and iFluor® dyes.
Proteases play essential roles in protein activation, cell regulation and signaling, as well as in the generation of amino acids for protein synthesis or utilization in other metabolic pathways. FRET protease substrates are widely used for detecting protease activities, in particular, for virus protease that often require a long peptide sequence for optimal binding such as coronavirus, HIV and HCV proteases. The internally quenched FRET peptide substrate is digested by a protease to generate the highly fluorescent peptide fragment. The fluorescence increase is proportional to the protease activity. Tide Quencher™ dyes have been proven to be the extremely effective quenchers for developing FRET protease substrates for high throughput screening applications together with our bright Tide Fluor™ and iFluor® dyes.
protocol
Protocol
COA
CatalogSize
Price
Quantity
13542100 tests
Price
135431000 tests
Price
 
Physical properties
Molecular weight3067.69
SolventDMSO
Spectral properties
Correction factor (260 nm)0.03
Correction factor (280 nm)0.03
Correction factor (656 nm)0.0793
Extinction coefficient (cm -1 M -1)
250000
1
Excitation (nm)656
Emission (nm)670
Quantum yield
0.25
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings
Fluorescence microplate reader
Excitation640 nm
Emission680 nm
Cutoff660 nm
Recommended plateSolid black
Documents
Protocol
Safety Data Sheet (Catalog 13542)
Safety Data Sheet (Catalog 13543)
Certificate of Analysis
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Page updated on October 20, 2025