CUG [3-Carboxyumbelliferyl beta-D-galactopyranoside] *CAS 64664-99-9*

CUG

3-Carboxyumbelliferyl beta-D-galactopyranoside; CAS 64664-99-9

CUG is a fluorogenic galactosidase substrate with carboxy reactive handle that can be used for conjugation. The organic and inorganic esters and glycosides of 7-hydroxycoumarin are among the most commonly used fluorogenic substrates. These substrates yield water-soluble blue fluorescent coumarin fluorophores upon interaction with enzymes.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
14003	10 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CUG [3-Carboxyumbelliferyl beta-D-galactopyranoside] *CAS 64664-99-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	271.525 µL	1.358 mL	2.715 mL	13.576 mL	27.153 mL
5 mM	54.305 µL	271.525 µL	543.05 µL	2.715 mL	5.431 mL
10 mM	27.153 µL	135.763 µL	271.525 µL	1.358 mL	2.715 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	368.29 g/mol	=	mL	x	mM	=	mmol

References

View all 9 references: Citation Explorer

The translation of an antiapoptotic protein HIAP2 is regulated by an upstream open reading frame

Authors:

Warnakulasuriyarachchi D, Ungureanu NH, Holcik M.

Journal:

Cell Death Differ (2003): 899

Absence of effect of varying Thr-Leu codon pairs on protein synthesis in a T7 system

Authors:

Cheng L, Goldman E.

Journal:

Biochemistry (2001): 6102

The CUG-binding protein binds specifically to UG dinucleotide repeats in a yeast three-hybrid system

Authors:

Takahashi N, Sasagawa N, Suzuki K, Ishiura S.

Journal:

Biochem Biophys Res Commun (2000): 518

An AUG initiation codon, not codon-anticodon complementarity, is required for the translation of unleadered mRNA in Escherichia coli

Authors:

Van Etten WJ, Janssen GR.

Journal:

Mol Microbiol (1998): 987

Molecular characterisation of the pifC gene encoding translation initiation factor 3, which is required for normal photosynthetic complex formation in Rhodobacter sphaeroides NCIB 8253

Authors:

Babic S, Hunter CN, Rakhlin NJ, Simons RW, Phillips-Jones MK.

Journal:

Eur J Biochem (1997): 564

Physical properties

Molecular weight	368.29
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.0815
Excitation (nm)	352
Emission (nm)	407

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	64664-99-9

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on July 12, 2023