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CUG [3-Carboxyumbelliferyl beta-D-galactopyranoside] *CAS 64664-99-9*
Ordering information
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
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| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 368.29 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (280 nm) | 0.0815 |
| Excitation (nm) | 352 |
| Emission (nm) | 407 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
| Overview |  SDSProtocol |
CAS 64664-99-9 | Molecular weight 368.29 | Correction Factor (280 nm) 0.0815 | Excitation (nm) 352 | Emission (nm) 407 |
CUG is a fluorogenic galactosidase substrate with carboxy reactive handle that can be used for conjugation. The organic and inorganic esters and glycosides of 7-hydroxycoumarin are among the most commonly used fluorogenic substrates. These substrates yield water-soluble blue fluorescent coumarin fluorophores upon interaction with enzymes.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of CUG [3-Carboxyumbelliferyl beta-D-galactopyranoside] *CAS 64664-99-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 271.525 µL | 1.358 mL | 2.715 mL | 13.576 mL | 27.153 mL |
| 5 mM | 54.305 µL | 271.525 µL | 543.05 µL | 2.715 mL | 5.431 mL |
| 10 mM | 27.153 µL | 135.763 µL | 271.525 µL | 1.358 mL | 2.715 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (280 nm) | 0.0815 |
| Excitation (nm) | 352 |
| Emission (nm) | 407 |
Images
Figure 1. Chemical structure for CUG [3-Carboxyumbelliferyl beta-D-galactopyranoside] *CAS 64664-99-9*
References
View all 9 references: Citation Explorer
The translation of an antiapoptotic protein HIAP2 is regulated by an upstream open reading frame
Authors: Warnakulasuriyarachchi D, Ungureanu NH, Holcik M.
Journal: Cell Death Differ (2003): 899
Authors: Warnakulasuriyarachchi D, Ungureanu NH, Holcik M.
Journal: Cell Death Differ (2003): 899
Absence of effect of varying Thr-Leu codon pairs on protein synthesis in a T7 system
Authors: Cheng L, Goldman E.
Journal: Biochemistry (2001): 6102
Authors: Cheng L, Goldman E.
Journal: Biochemistry (2001): 6102
The CUG-binding protein binds specifically to UG dinucleotide repeats in a yeast three-hybrid system
Authors: Takahashi N, Sasagawa N, Suzuki K, Ishiura S.
Journal: Biochem Biophys Res Commun (2000): 518
Authors: Takahashi N, Sasagawa N, Suzuki K, Ishiura S.
Journal: Biochem Biophys Res Commun (2000): 518
An AUG initiation codon, not codon-anticodon complementarity, is required for the translation of unleadered mRNA in Escherichia coli
Authors: Van Etten WJ, Janssen GR.
Journal: Mol Microbiol (1998): 987
Authors: Van Etten WJ, Janssen GR.
Journal: Mol Microbiol (1998): 987
Fibroblast growth factor 3, a protein with dual subcellular localization, is targeted to the nucleus and nucleolus by the concerted action of two nuclear localization signals and a nucleolar retention signal
Authors: Antoine M, Reimers K, Dickson C, Kiefer P.
Journal: J Biol Chem (1997): 29475
Authors: Antoine M, Reimers K, Dickson C, Kiefer P.
Journal: J Biol Chem (1997): 29475
A carboxyl-terminal domain in fibroblast growth factor (FGF)-2 inhibits FGF-1 release in response to heat shock in vitro
Authors: Shi J, Friedman S, Maciag T.
Journal: J Biol Chem (1997): 1142
Authors: Shi J, Friedman S, Maciag T.
Journal: J Biol Chem (1997): 1142
Molecular characterisation of the pifC gene encoding translation initiation factor 3, which is required for normal photosynthetic complex formation in Rhodobacter sphaeroides NCIB 8253
Authors: Babic S, Hunter CN, Rakhlin NJ, Simons RW, Phillips-Jones MK.
Journal: Eur J Biochem (1997): 564
Authors: Babic S, Hunter CN, Rakhlin NJ, Simons RW, Phillips-Jones MK.
Journal: Eur J Biochem (1997): 564
Increased ribosomal accuracy increases a programmed translational frameshift in Escherichia coli
Authors: Sipley J, Goldman E.
Journal: Proc Natl Acad Sci U S A (1993): 2315
Authors: Sipley J, Goldman E.
Journal: Proc Natl Acad Sci U S A (1993): 2315
Effect of structure of the initiator codon on translation in E. coli
Authors: Khudyakov Yu E, Neplyueva VS, Kalinina TI, Smirnov VD.
Journal: FEBS Lett (1988): 369
Authors: Khudyakov Yu E, Neplyueva VS, Kalinina TI, Smirnov VD.
Journal: FEBS Lett (1988): 369