Cy2DIGE NHS ester
Cy2DIGE NHS ester, an equivalent of Cy2® NHS ester minimal dye, is used for labeling proteins subject to DIGE analysis. Cy2DIGE is commonly used as a reference color together with C3DIGE and/or Cy5DIGE. Cy2DIGE, C3DIGE and Cy5DIGE are specially designed for precise comparison of protein expression in two or three samples of lysates. Perfect mobility matching and bright dye fluorescence allow the efficient detection and high resolution of minor proteins on 2D gel electrophoresis. Cy2DIGE, C3DIGE and Cy5DIGE are compatible to all imagers capable of detection of Cy2, Cy3, and Cy5 dyes. The gels labeled with Cy2DIGE, C3DIGE and Cy5DIGE are size- and charge-matched fluorescent dyes for detecting protein abundance differences in 2-D Fluorescence Difference Gel Electrophoresis (DIGE). Cy2DIGE, C3DIGE and Cy5DIGE detect up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel. The size- and charge-matched dyes enable co-migration of labeled samples within the gel. They are bright on proteins, allowing the minimal loss of signal during labeling, separation, and scanning. These dyes have minimal spectral overlap, minimizing the crosstalk that commonly contributes to high background. Their pH-insensitive fluorescence allows DIGEs run at a broad pH range. Cy2®, Cy3® and Cy5® are the trademarks of GE Healthcare.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cy2DIGE NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 147.601 µL | 738.007 µL | 1.476 mL | 7.38 mL | 14.76 mL |
5 mM | 29.52 µL | 147.601 µL | 295.203 µL | 1.476 mL | 2.952 mL |
10 mM | 14.76 µL | 73.801 µL | 147.601 µL | 738.007 µL | 1.476 mL |
Molarity calculator
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Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester* | 499 | 520 | 71000 | 0.30 | 0.11 |
XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester* | 343 | 441 | 19000 | 0.25 | 0.19 |
XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester* | 534 | 553 | 81000 | 0.24 | 0.09 |
XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester* | 590 | 618 | 90000 | 0.43 | 0.56 |
QXY21 NHS ester [equivalent to QSY-21 NHS ester] | - | - | 890001 | - | 0.32 |
XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester* | 553 | 568 | 150000 | 0.08 | 0.08 |
XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester* | 650 | 671 | 239000 | 0.00 | 0.03 |
XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester* | 681 | 704 | 184000 | 0.00 | 0.05 |
XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester* | 696 | 719 | 192000 | 0.00 | 0.07 |
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Citations
View all 34 citations: Citation Explorer
LncRNA AL592284. 1 facilitates proliferation and metastasis of cervical cancer cells via miR-30a-5p/Vimentin/EMT axis
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
Early quantitative profiling of differential retinal protein expression in lens-induced myopia in guinea pig using fluorescence difference two-dimensional gel electrophoresis
Authors: Wu, Y., Lam, C. S., Tse, D. Y., To, C. H., Liu, Q., McFadden, S. A., Chun, R. K., Li, K. K., Bian, J., Lam, C.
Journal: Mol Med Rep (2018): 5571-5580
Authors: Wu, Y., Lam, C. S., Tse, D. Y., To, C. H., Liu, Q., McFadden, S. A., Chun, R. K., Li, K. K., Bian, J., Lam, C.
Journal: Mol Med Rep (2018): 5571-5580
Proteomic Analysis of Bovine Pregnancy-specific Serum Proteins by 2D Fluorescence Difference Gel Electrophoresis
Authors: Lee, J. E., Lee, J. Y., Kim, H. R., Shin, H. Y., Lin, T., Jin, D. I.
Journal: Asian-Australas J Anim Sci (2015): 788-95
Authors: Lee, J. E., Lee, J. Y., Kim, H. R., Shin, H. Y., Lin, T., Jin, D. I.
Journal: Asian-Australas J Anim Sci (2015): 788-95
Comparative proteomic analysis of different Toxoplasma gondii genotypes by two-dimensional fluorescence difference gel electrophoresis combined with mass spectrometry
Authors: Zhou, D. H., Zhao, F. R., Nisbet, A. J., Xu, M. J., Song, H. Q., Lin, R. Q., Huang, S. Y., Zhu, X. Q.
Journal: Electrophoresis (2014): 533-45
Authors: Zhou, D. H., Zhao, F. R., Nisbet, A. J., Xu, M. J., Song, H. Q., Lin, R. Q., Huang, S. Y., Zhu, X. Q.
Journal: Electrophoresis (2014): 533-45
Comparative proteomic analysis of Dan'er malts produced from distinct malting processes by two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE)
Authors: Li, X., Jin, Z., Gao, F., Lu, J., Cai, G., Dong, J., Yu, J., Yang, M.
Journal: J Agric Food Chem (2014): 9310-6
Authors: Li, X., Jin, Z., Gao, F., Lu, J., Cai, G., Dong, J., Yu, J., Yang, M.
Journal: J Agric Food Chem (2014): 9310-6
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