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Cy3.5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*

ABI 7500 FAST system Cy3.5 dye spectrum
ABI 7500 FAST system Cy3.5 dye spectrum
ABI 7500 FAST system Cy3.5 dye spectrum
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Physical properties
Molecular weightN/A
Spectral properties
Correction Factor (260 nm)0.08
Correction Factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)150000
Excitation (nm)579
Emission (nm)591
Quantum yield0.15
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
Cy3.5 Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR System with a Fast 96-well block. For most qPCR instruments, it is necessary to calibrate the device at least every six months. This calibration plate is ready to use without any additional preparation steps. The qPCR calibration plate might significantly improve qPCR results with multiplexing by more accurately representing fluorescent spectra used in your real-time experiments. Please refer to your instrument's guide for the detailed calibration operation.


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Spectral properties

Correction Factor (260 nm)0.08
Correction Factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)150000
Excitation (nm)579
Emission (nm)591
Quantum yield0.15



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