Cy3.5 Dye qPCR Calibration Plate Optimized for ABI7500 Fast 96-Well

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	N/A
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.08
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	150000
Excitation (nm)	579
Emission (nm)	591
Quantum yield	0.15

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Molecular weight

N/A

Correction Factor (260 nm)

0.08

Correction Factor (280 nm)

0.178

Extinction coefficient (cm ^-1 M ^-1)

150000

Excitation (nm)

579

Emission (nm)

591

Quantum yield

0.15

Cy3.5 Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR System with a Fast 96-well block. For most qPCR instruments, it is necessary to calibrate the device at least every six months. This calibration plate is ready to use without any additional preparation steps. The qPCR calibration plate might significantly improve qPCR results with multiplexing by more accurately representing fluorescent spectra used in your real-time experiments. Please refer to your instrument's guide for the detailed calibration operation.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.08
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	150000
Excitation (nm)	579
Emission (nm)	591
Quantum yield	0.15

Images

Figure 1. ABI 7500 FAST system Cy3.5 dye spectrum

References

View all 50 references: Citation Explorer

Development of a rapid qPCR method to quantify lactic acid bacteria in cold-smoked salmon.
Authors: Jérôme, Marc and Passerini, Delphine and Chevalier, Frédérique and Marchand, Laetitia and Leroi, Françoise and Macé, Sabrina
Journal: International journal of food microbiology (2022): 109504

Semi-Quantitative Detection of Drosophila suzukii (Diptera: Drosophilidae) From Bulk Trap Samples Using PCR Technology.
Authors: Renkema, Justin M and McFadden-Smith, Wendy and Chen, Shu
Journal: Journal of economic entomology (2022)

Continuous polymerase chain reaction microfluidics integrated with a gold-capped nanoslit sensing chip for Epstein-Barr virus detection.
Authors: Hsieh, Han-Yun and Chang, Ray and Huang, Yung-Yu and Juan, Po-Han and Tahara, Hidetoshi and Lee, Kuan-Yi and Vo, Di Ngoc Kha and Tsai, Ming-Han and Wei, Pei-Kuen and Sheen, Horn-Jiunn and Fan, Yu-Jui
Journal: Biosensors & bioelectronics (2022): 113672

XCL1 Aggravates Diabetic Nephropathy-Mediated Renal Glomerular Endothelial Cell Apoptosis and Inflammatory Response via Regulating p53/Nuclear Factor-Kappa B Pathway.
Authors: Zhang, Yuan and Chen, Xiaolan and Fan, Yaping and Liu, Jing and Yuan, Li
Journal: Nephron (2022): 84-98

DNA-based quantification of Fusarium oxysporum f. sp. vasinfectum in environmental soils to describe spatial variation in inoculum density.
Authors: Davis, Roy and Isakeit, Thomas and Chappell, Thomas
Journal: Plant disease (2022)

First case of SARS-CoV-2 RNA detection in municipal solid waste leachate from Brazil.
Authors: Mondelli, Giulliana and Silva, Ednei Rodrigues and Claro, Ieda Carolina Mantovani and Augusto, Matheus Ribeiro and Duran, Adriana Feliciano Alves and Cabral, Aline Diniz and de Moraes Bomediano Camillo, Lívia and Dos Santos Oliveira, Luísa Helena and de Freitas Bueno, Rodrigo
Journal: The Science of the total environment (2022): 153927

Precise RNA Quantification by Counting Individual RNA Molecules Using High-Sensitivity Capillary Flow Cytometry.
Authors: Yoo, Hee-Bong and Park, Sang-Ryoul and Hong, Kee-Suk and Yang, Inchul
Journal: Analytical chemistry (2022): 1752-1759

Accurate qPCR quantification in polymicrobial communities requires assessment of gDNA extraction efficiency.
Authors: Cerca, Nuno and Lima, Ângela and França, Angela
Journal: Journal of microbiological methods (2022): 106421

Lentiviral standards to determine the sensitivity of assays that quantify lentiviral vector copy numbers and genomic insertion sites in cells.
Authors: Corre, Guillaume and Seye, Ababacar and Frin, Sophie and Ferrand, Maxime and Winkler, Kathrin and Luc, Cyril and Dorange, Fabien and Rocca, Céline J and Galy, Anne
Journal: Gene therapy (2022)

One-Step Multiplexed Droplet Digital Polymerase Chain Reaction for Quantification of p190 BCR-ABL1 Fusion Transcript in B-Lymphoblastic Leukemia.
Authors: Martinez, Ryan J and Kang, Qing and Nennig, Davis and Bailey, Nathanael G and Brown, Noah A and Betz, Bryan L and Tewari, Muneesh and Thyagarajan, Bharat and Bachanova, Veronika and Mroz, Pawel
Journal: Archives of pathology & laboratory medicine (2022): 92-100