Cy3 biotin conjugate

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1239.42
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.07
Correction Factor (280 nm)	0.073
Extinction coefficient (cm ^-1 M ^-1)	150000¹
Excitation (nm)	555
Emission (nm)	569
Quantum yield	0.15¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

1239.42

Correction Factor (260 nm)

0.07

Correction Factor (280 nm)

0.073

Extinction coefficient (cm ^-1 M ^-1)

150000¹

Excitation (nm)

555

Emission (nm)

569

Quantum yield

0.15¹

This bifunctional Cy3 biotin conjugate demonstrates excellent avidin binding and strong fluorescence upon binding to a protein or nucleic acid target. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. Cy3 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and emission. Cy3 Biotin is a water-soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cy3 biotin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	80.683 µL	403.415 µL	806.829 µL	4.034 mL	8.068 mL
5 mM	16.137 µL	80.683 µL	161.366 µL	806.829 µL	1.614 mL
10 mM	8.068 µL	40.341 µL	80.683 µL	403.415 µL	806.829 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.07
Correction Factor (280 nm)	0.073
Extinction coefficient (cm ^-1 M ^-1)	150000¹
Excitation (nm)	555
Emission (nm)	569
Quantum yield	0.15¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Cy3®-streptavidin conjugate	555	569	150000¹	0.15¹	0.07	0.073
Cy5 biotin conjugate	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
Cy7 biotin conjugate	756	779	250000	0.3	0.05	0.036

Images

Figure 1. Chemical structure for Cy3 biotin conjugate.

References

View all 50 references: Citation Explorer

A low-noise ratiometric fluorescence biosensor for detection of Pb2+ based on DNAzyme and exonuclease III-assisted cascade signal amplification.
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Journal: Analytical and bioanalytical chemistry (2022): 1899-1907

In Vitro and In Situ Activity-Based Labeling of Fibroblast Activation Protein with UAMC1110-Derived Probes.
Authors: Van Rymenant, Yentl and Tanc, Muhammet and Van Elzen, Roos and Bracke, An and De Wever, Olivier and Augustyns, Koen and Lambeir, Anne-Marie and Kockx, Mark and De Meester, Ingrid and Van Der Veken, Pieter
Journal: Frontiers in chemistry (2021): 640566

Multicolor fluorescence encoding of different microRNAs in lung cancer tissues at the single-molecule level.
Authors: Li, Chen-Chen and Chen, Hui-Yan and Luo, Xiliang and Hu, Juan and Zhang, Chun-Yang
Journal: Chemical science (2021): 12407-12418

Global Detection of Proteins by Label-Based Antibody Array.
Authors: Kuang, Zhizhou and Chen, Li-Pai and Huang, Ruochun and Huang, Ruo-Pan
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 45-53

The poly-thymine based DNA photolithography onto electrostatic coupling substrates.
Authors: Song, Youngjun
Journal: Materials science & engineering. C, Materials for biological applications (2020): 110795

Incorporation of isotopic, fluorescent, and heavy-atom-modified nucleotides into RNAs by position-selective labeling of RNA.
Authors: Liu, Yu and Holmstrom, Erik and Yu, Ping and Tan, Kemin and Zuo, Xiaobing and Nesbitt, David J and Sousa, Rui and Stagno, Jason R and Wang, Yun-Xing
Journal: Nature protocols (2018): 987-1005

Evaluation of Matrix Metalloproteinase Inhibition by Peptide Microarray-Based Fluorescence Assay on Polymer Brush Substrate and in Vivo Assessment.
Authors: Lei, Zhen and Chen, Hongda and Zhang, Hua and Wang, Yaoqi and Meng, Xianying and Wang, Zhenxin
Journal: ACS applied materials & interfaces (2017): 44241-44250

Rapid visual identification of PCR amplified nucleic acids by centrifugal gel separation: Potential use for molecular point-of-care tests.
Authors: Hwang, Sang-Hyun and Kim, Dong-Eun and Im, Ji-Hyun and Kang, Su-Jin and Lee, Do-Hoon and Son, Sang Jun
Journal: Biosensors & bioelectronics (2016): 829-34

Microarrays as Model Biosensor Platforms to Investigate the Structure and Affinity of Aptamers.
Authors: Martin, Jennifer A and Chushak, Yaroslav and Chávez, Jorge L and Hagen, Joshua A and Kelley-Loughnane, Nancy
Journal: Journal of nucleic acids (2016): 9718612

Applications of PLOR in labeling large RNAs at specific sites.
Authors: Liu, Yu and Yu, Ping and Dyba, Marzena and Sousa, Rui and Stagno, Jason R and Wang, Yun-Xing
Journal: Methods (San Diego, Calif.) (2016): 4-10