Name: Cy3 biotin conjugate
Brand: AAT Bioquest
SKU: 3099
Price: 208 USD
Availability: InStock

Cy3 biotin conjugate

This bifunctional Cy3 biotin conjugate demonstrates excellent avidin binding and strong fluorescence upon binding to a protein or nucleic acid target. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. Cy3 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and emission. Cy3 Biotin is a water-soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
3099	5 mg	Price

References

View all 50 references: Citation Explorer

A low-noise ratiometric fluorescence biosensor for detection of Pb2+ based on DNAzyme and exonuclease III-assisted cascade signal amplification.

Authors:

Jin, Huali and Liu, Ruike and Bai, Tian and Wei, Min and He, Baoshan and Suo, Zhiguang

Journal:

Analytical and bioanalytical chemistry (2022): 1899-1907

In Vitro and In Situ Activity-Based Labeling of Fibroblast Activation Protein with UAMC1110-Derived Probes.

Authors:

Van Rymenant, Yentl and Tanc, Muhammet and Van Elzen, Roos and Bracke, An and De Wever, Olivier and Augustyns, Koen and Lambeir, Anne-Marie and Kockx, Mark and De Meester, Ingrid and Van Der Veken, Pieter

Journal:

Frontiers in chemistry (2021): 640566

Multicolor fluorescence encoding of different microRNAs in lung cancer tissues at the single-molecule level.

Authors:

Li, Chen-Chen and Chen, Hui-Yan and Luo, Xiliang and Hu, Juan and Zhang, Chun-Yang

Journal:

Chemical science (2021): 12407-12418

Global Detection of Proteins by Label-Based Antibody Array.

Authors:

Kuang, Zhizhou and Chen, Li-Pai and Huang, Ruochun and Huang, Ruo-Pan

Journal:

Methods in molecular biology (Clifton, N.J.) (2021): 45-53

The poly-thymine based DNA photolithography onto electrostatic coupling substrates.

Authors:

Song, Youngjun

Journal:

Materials science & engineering. C, Materials for biological applications (2020): 110795

Physical properties

Molecular weight	1239.42
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.07
Correction factor (280 nm)	0.073
Extinction coefficient (cm ^-1 M ^-1)	150000 ¹
Excitation (nm)	555
Emission (nm)	569
Quantum yield	0.15 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 13, 2025