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Cy3 biotin conjugate
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1239.42 |
| Solvent | Water |
Spectral properties
| Correction Factor (260 nm) | 0.07 |
| Correction Factor (280 nm) | 0.073 |
| Extinction coefficient (cm -1 M -1) | 1500001 |
| Excitation (nm) | 555 |
| Emission (nm) | 569 |
| Quantum yield | 0.151 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Related products
| Overview |  SDSProtocol |
See also: Cyanines
Molecular weight 1239.42 | Correction Factor (260 nm) 0.07 | Correction Factor (280 nm) 0.073 | Extinction coefficient (cm -1 M -1) 1500001 | Excitation (nm) 555 | Emission (nm) 569 | Quantum yield 0.151 |
This bifunctional Cy3 biotin conjugate demonstrates excellent avidin binding and strong fluorescence upon binding to a protein or nucleic acid target. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. Cy3 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and emission. Cy3 Biotin is a water-soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Cy3 biotin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 80.683 µL | 403.415 µL | 806.829 µL | 4.034 mL | 8.068 mL |
| 5 mM | 16.137 µL | 80.683 µL | 161.366 µL | 806.829 µL | 1.614 mL |
| 10 mM | 8.068 µL | 40.341 µL | 80.683 µL | 403.415 µL | 806.829 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.07 |
| Correction Factor (280 nm) | 0.073 |
| Extinction coefficient (cm -1 M -1) | 1500001 |
| Excitation (nm) | 555 |
| Emission (nm) | 569 |
| Quantum yield | 0.151 |
Images
Figure 1. Chemical structure for Cy3 biotin conjugate.
References
View all 50 references: Citation Explorer
A low-noise ratiometric fluorescence biosensor for detection of Pb2+ based on DNAzyme and exonuclease III-assisted cascade signal amplification.
Authors: Jin, Huali and Liu, Ruike and Bai, Tian and Wei, Min and He, Baoshan and Suo, Zhiguang
Journal: Analytical and bioanalytical chemistry (2022): 1899-1907
Authors: Jin, Huali and Liu, Ruike and Bai, Tian and Wei, Min and He, Baoshan and Suo, Zhiguang
Journal: Analytical and bioanalytical chemistry (2022): 1899-1907
In Vitro and In Situ Activity-Based Labeling of Fibroblast Activation Protein with UAMC1110-Derived Probes.
Authors: Van Rymenant, Yentl and Tanc, Muhammet and Van Elzen, Roos and Bracke, An and De Wever, Olivier and Augustyns, Koen and Lambeir, Anne-Marie and Kockx, Mark and De Meester, Ingrid and Van Der Veken, Pieter
Journal: Frontiers in chemistry (2021): 640566
Authors: Van Rymenant, Yentl and Tanc, Muhammet and Van Elzen, Roos and Bracke, An and De Wever, Olivier and Augustyns, Koen and Lambeir, Anne-Marie and Kockx, Mark and De Meester, Ingrid and Van Der Veken, Pieter
Journal: Frontiers in chemistry (2021): 640566
Multicolor fluorescence encoding of different microRNAs in lung cancer tissues at the single-molecule level.
Authors: Li, Chen-Chen and Chen, Hui-Yan and Luo, Xiliang and Hu, Juan and Zhang, Chun-Yang
Journal: Chemical science (2021): 12407-12418
Authors: Li, Chen-Chen and Chen, Hui-Yan and Luo, Xiliang and Hu, Juan and Zhang, Chun-Yang
Journal: Chemical science (2021): 12407-12418
Global Detection of Proteins by Label-Based Antibody Array.
Authors: Kuang, Zhizhou and Chen, Li-Pai and Huang, Ruochun and Huang, Ruo-Pan
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 45-53
Authors: Kuang, Zhizhou and Chen, Li-Pai and Huang, Ruochun and Huang, Ruo-Pan
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 45-53
The poly-thymine based DNA photolithography onto electrostatic coupling substrates.
Authors: Song, Youngjun
Journal: Materials science & engineering. C, Materials for biological applications (2020): 110795
Authors: Song, Youngjun
Journal: Materials science & engineering. C, Materials for biological applications (2020): 110795
Incorporation of isotopic, fluorescent, and heavy-atom-modified nucleotides into RNAs by position-selective labeling of RNA.
Authors: Liu, Yu and Holmstrom, Erik and Yu, Ping and Tan, Kemin and Zuo, Xiaobing and Nesbitt, David J and Sousa, Rui and Stagno, Jason R and Wang, Yun-Xing
Journal: Nature protocols (2018): 987-1005
Authors: Liu, Yu and Holmstrom, Erik and Yu, Ping and Tan, Kemin and Zuo, Xiaobing and Nesbitt, David J and Sousa, Rui and Stagno, Jason R and Wang, Yun-Xing
Journal: Nature protocols (2018): 987-1005
Evaluation of Matrix Metalloproteinase Inhibition by Peptide Microarray-Based Fluorescence Assay on Polymer Brush Substrate and in Vivo Assessment.
Authors: Lei, Zhen and Chen, Hongda and Zhang, Hua and Wang, Yaoqi and Meng, Xianying and Wang, Zhenxin
Journal: ACS applied materials & interfaces (2017): 44241-44250
Authors: Lei, Zhen and Chen, Hongda and Zhang, Hua and Wang, Yaoqi and Meng, Xianying and Wang, Zhenxin
Journal: ACS applied materials & interfaces (2017): 44241-44250
Rapid visual identification of PCR amplified nucleic acids by centrifugal gel separation: Potential use for molecular point-of-care tests.
Authors: Hwang, Sang-Hyun and Kim, Dong-Eun and Im, Ji-Hyun and Kang, Su-Jin and Lee, Do-Hoon and Son, Sang Jun
Journal: Biosensors & bioelectronics (2016): 829-34
Authors: Hwang, Sang-Hyun and Kim, Dong-Eun and Im, Ji-Hyun and Kang, Su-Jin and Lee, Do-Hoon and Son, Sang Jun
Journal: Biosensors & bioelectronics (2016): 829-34
Microarrays as Model Biosensor Platforms to Investigate the Structure and Affinity of Aptamers.
Authors: Martin, Jennifer A and Chushak, Yaroslav and Chávez, Jorge L and Hagen, Joshua A and Kelley-Loughnane, Nancy
Journal: Journal of nucleic acids (2016): 9718612
Authors: Martin, Jennifer A and Chushak, Yaroslav and Chávez, Jorge L and Hagen, Joshua A and Kelley-Loughnane, Nancy
Journal: Journal of nucleic acids (2016): 9718612
Applications of PLOR in labeling large RNAs at specific sites.
Authors: Liu, Yu and Yu, Ping and Dyba, Marzena and Sousa, Rui and Stagno, Jason R and Wang, Yun-Xing
Journal: Methods (San Diego, Calif.) (2016): 4-10
Authors: Liu, Yu and Yu, Ping and Dyba, Marzena and Sousa, Rui and Stagno, Jason R and Wang, Yun-Xing
Journal: Methods (San Diego, Calif.) (2016): 4-10