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Cy3 biotin conjugate

This bifunctional Cy3 biotin conjugate demonstrates excellent avidin binding and strong fluorescence upon binding to a protein or nucleic acid target. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. Cy3 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and emission. Cy3 Biotin is a water-soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG spacer between biotin moiety and fluorescent tag minimizes the steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Spectrum

References

View all 50 references: Citation Explorer
A low-noise ratiometric fluorescence biosensor for detection of Pb2+ based on DNAzyme and exonuclease III-assisted cascade signal amplification.
Authors: Jin, Huali and Liu, Ruike and Bai, Tian and Wei, Min and He, Baoshan and Suo, Zhiguang
Journal: Analytical and bioanalytical chemistry (2022): 1899-1907
In Vitro and In Situ Activity-Based Labeling of Fibroblast Activation Protein with UAMC1110-Derived Probes.
Authors: Van Rymenant, Yentl and Tanc, Muhammet and Van Elzen, Roos and Bracke, An and De Wever, Olivier and Augustyns, Koen and Lambeir, Anne-Marie and Kockx, Mark and De Meester, Ingrid and Van Der Veken, Pieter
Journal: Frontiers in chemistry (2021): 640566
Multicolor fluorescence encoding of different microRNAs in lung cancer tissues at the single-molecule level.
Authors: Li, Chen-Chen and Chen, Hui-Yan and Luo, Xiliang and Hu, Juan and Zhang, Chun-Yang
Journal: Chemical science (2021): 12407-12418
Global Detection of Proteins by Label-Based Antibody Array.
Authors: Kuang, Zhizhou and Chen, Li-Pai and Huang, Ruochun and Huang, Ruo-Pan
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 45-53
The poly-thymine based DNA photolithography onto electrostatic coupling substrates.
Authors: Song, Youngjun
Journal: Materials science & engineering. C, Materials for biological applications (2020): 110795
Page updated on November 12, 2024

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Catalog Number3099
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Physical properties

Molecular weight

1239.42

Solvent

Water

Spectral properties

Correction Factor (260 nm)

0.07

Correction Factor (280 nm)

0.073

Extinction coefficient (cm -1 M -1)

1500001

Excitation (nm)

555

Emission (nm)

569

Quantum yield

0.151

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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