Cy3B azide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	684.86
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.048
Correction Factor (280 nm)	0.069
Extinction coefficient (cm ^-1 M ^-1)	120000¹
Excitation (nm)	560
Emission (nm)	571
Quantum yield	0.58¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Direct upgrades

Alternative formats

Overview SDS Protocol

Upgrade: iFluor® 555 azide

Molecular weight

684.86

Correction Factor (260 nm)

0.048

Correction Factor (280 nm)

0.069

Extinction coefficient (cm ^-1 M ^-1)

120000¹

Excitation (nm)

560

Emission (nm)

571

Quantum yield

0.58¹

Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. However, Cy3 suffers low fluorescence intensity. It is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3B azide can be used for conjugations with alkyne-containing biological molecules. Cy3® is the trademark of GE Healthcare.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3B azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	146.015 µL	730.076 µL	1.46 mL	7.301 mL	14.602 mL
5 mM	29.203 µL	146.015 µL	292.03 µL	1.46 mL	2.92 mL
10 mM	14.602 µL	73.008 µL	146.015 µL	730.076 µL	1.46 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.048
Correction Factor (280 nm)	0.069
Extinction coefficient (cm ^-1 M ^-1)	120000¹
Excitation (nm)	560
Emission (nm)	571
Quantum yield	0.58¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
Cy3B DBCO	560	571	120000¹	0.069
Cy3B TCO	560	571	120000¹	0.069
Cy3B Tetrazine	560	571	120000¹	0.069
AMCA Azide	346	434	19000	0.153
XFD488 azide Same Structure to Alexa Fluor™ 488 azide	490	520	71000	0.11
ICG azide	789	814	-	0.076
XFD647 Azide	650	671	270000	0.03

Images

Figure 1. Chemical structure for Cy3B azide.

References

View all 37 references: Citation Explorer

Fluorescence Anisotropy-Based Assay for Characterization of Ligand Binding Dynamics to GPCRs: The Case of Cy3B-Labeled Ligands Binding to MC4 Receptors in Budded Baculoviruses.
Authors: Veiksina, Santa and Tahk, Maris-Johanna and Laasfeld, Tõnis and Link, Reet and Kopanchuk, Sergei and Rinken, Ago
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 119-136

Quantitative analysis of fluorescent ligand binding to dopamine D3 receptors using live-cell microscopy.
Authors: Allikalt, Anni and Laasfeld, Tõnis and Ilisson, Mihkel and Kopanchuk, Sergei and Rinken, Ago
Journal: The FEBS journal (2021): 1514-1532

Fluorescent ligands for dopamine D2/D3 receptors.
Authors: Allikalt, Anni and Purkayastha, Nirupam and Flad, Khajidmaa and Schmidt, Maximilian F and Tabor, Alina and Gmeiner, Peter and Hübner, Harald and Weikert, Dorothee
Journal: Scientific reports (2020): 21842

Unifying Mechanism for Thiol-Induced Photoswitching and Photostability of Cyanine Dyes.
Authors: Gidi, Yasser and Payne, Liam and Glembockyte, Viktorija and Michie, Megan S and Schnermann, Martin J and Cosa, Gonzalo
Journal: Journal of the American Chemical Society (2020): 12681-12689

Fluorescence lifetime imaging with a single-photon SPAD array using long overlapping gates: an experimental and theoretical study.
Authors: Ardelean, Andrei and Ulku, Arin Can and Michalet, Xavier and Charbon, Edoardo and Bruschini, Claudio
Journal: Proceedings of SPIE--the International Society for Optical Engineering (2019)

Redox-Based Photostabilizing Agents in Fluorescence Imaging: The Hidden Role of Intersystem Crossing in Geminate Radical Ion Pairs.
Authors: Glembockyte, Viktorija and Cosa, Gonzalo
Journal: Journal of the American Chemical Society (2017): 13227-13233

Characterization of ligand binding to melanocortin 4 receptors using fluorescent peptides with improved kinetic properties.
Authors: Link, Reet and Veiksina, Santa and Rinken, Ago and Kopanchuk, Sergei
Journal: European journal of pharmacology (2017): 58-66

Predicting signatures of anisotropic resonance energy transfer in dye-functionalized nanoparticles.
Authors: Gil, Gabriel and Corni, Stefano and Delgado, Alain and Bertoni, Andrea and Goldoni, Guido
Journal: RSC advances (2016): 104648-104656

Homogeneous fluorescence anisotropy-based assay for characterization of ligand binding dynamics to GPCRs in budded baculoviruses: the case of Cy3B-NDP-α-MSH binding to MC4 receptors.
Authors: Veiksina, Santa and Kopanchuk, Sergei and Mazina, Olga and Link, Reet and Lille, Anne and Rinken, Ago
Journal: Methods in molecular biology (Clifton, N.J.) (2015): 37-50

Cholesterol-induced lipophobic interaction between transmembrane helices using ensemble and single-molecule fluorescence resonance energy transfer.
Authors: Yano, Yoshiaki and Kondo, Kotaro and Kitani, Ryota and Yamamoto, Arisa and Matsuzaki, Katsumi
Journal: Biochemistry (2015): 1371-9