Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>
Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>
logo
AAT Bioquest

Cy3B azide

Product Image
Product Image
Gallery Image 1
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight684.86
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.048
Correction Factor (280 nm)0.069
Extinction coefficient (cm -1 M -1)1200001
Excitation (nm)560
Emission (nm)571
Quantum yield0.581
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Direct upgrades
iFluor® 555 azide

OverviewpdfSDSpdfProtocol


Molecular weight
684.86
Correction Factor (260 nm)
0.048
Correction Factor (280 nm)
0.069
Extinction coefficient (cm -1 M -1)
1200001
Excitation (nm)
560
Emission (nm)
571
Quantum yield
0.581
Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. However, Cy3 suffers low fluorescence intensity. It is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3B azide can be used for conjugations with alkyne-containing biological molecules. Cy3® is the trademark of GE Healthcare.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3B azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM146.015 µL730.076 µL1.46 mL7.301 mL14.602 mL
5 mM29.203 µL146.015 µL292.03 µL1.46 mL2.92 mL
10 mM14.602 µL73.008 µL146.015 µL730.076 µL1.46 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.048
Correction Factor (280 nm)0.069
Extinction coefficient (cm -1 M -1)1200001
Excitation (nm)560
Emission (nm)571
Quantum yield0.581

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)
Cy3B DBCO56057112000010.5810.069
Cy3B TCO56057112000010.5810.069
Cy3B Tetrazine56057112000010.5810.069
AMCA Azide34643419000-0.153
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide*499520730000.921-
ICG azide789814--0.076
XFD647 Azide6506712700000.3310.03

Images


References


View all 37 references: Citation Explorer
Fluorescence Anisotropy-Based Assay for Characterization of Ligand Binding Dynamics to GPCRs: The Case of Cy3B-Labeled Ligands Binding to MC4 Receptors in Budded Baculoviruses.
Authors: Veiksina, Santa and Tahk, Maris-Johanna and Laasfeld, Tõnis and Link, Reet and Kopanchuk, Sergei and Rinken, Ago
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 119-136
Quantitative analysis of fluorescent ligand binding to dopamine D3 receptors using live-cell microscopy.
Authors: Allikalt, Anni and Laasfeld, Tõnis and Ilisson, Mihkel and Kopanchuk, Sergei and Rinken, Ago
Journal: The FEBS journal (2021): 1514-1532
Fluorescent ligands for dopamine D2/D3 receptors.
Authors: Allikalt, Anni and Purkayastha, Nirupam and Flad, Khajidmaa and Schmidt, Maximilian F and Tabor, Alina and Gmeiner, Peter and Hübner, Harald and Weikert, Dorothee
Journal: Scientific reports (2020): 21842
Unifying Mechanism for Thiol-Induced Photoswitching and Photostability of Cyanine Dyes.
Authors: Gidi, Yasser and Payne, Liam and Glembockyte, Viktorija and Michie, Megan S and Schnermann, Martin J and Cosa, Gonzalo
Journal: Journal of the American Chemical Society (2020): 12681-12689
Fluorescence lifetime imaging with a single-photon SPAD array using long overlapping gates: an experimental and theoretical study.
Authors: Ardelean, Andrei and Ulku, Arin Can and Michalet, Xavier and Charbon, Edoardo and Bruschini, Claudio
Journal: Proceedings of SPIE--the International Society for Optical Engineering (2019)
Redox-Based Photostabilizing Agents in Fluorescence Imaging: The Hidden Role of Intersystem Crossing in Geminate Radical Ion Pairs.
Authors: Glembockyte, Viktorija and Cosa, Gonzalo
Journal: Journal of the American Chemical Society (2017): 13227-13233
Characterization of ligand binding to melanocortin 4 receptors using fluorescent peptides with improved kinetic properties.
Authors: Link, Reet and Veiksina, Santa and Rinken, Ago and Kopanchuk, Sergei
Journal: European journal of pharmacology (2017): 58-66
Predicting signatures of anisotropic resonance energy transfer in dye-functionalized nanoparticles.
Authors: Gil, Gabriel and Corni, Stefano and Delgado, Alain and Bertoni, Andrea and Goldoni, Guido
Journal: RSC advances (2016): 104648-104656
Homogeneous fluorescence anisotropy-based assay for characterization of ligand binding dynamics to GPCRs in budded baculoviruses: the case of Cy3B-NDP-α-MSH binding to MC4 receptors.
Authors: Veiksina, Santa and Kopanchuk, Sergei and Mazina, Olga and Link, Reet and Lille, Anne and Rinken, Ago
Journal: Methods in molecular biology (Clifton, N.J.) (2015): 37-50
Cholesterol-induced lipophobic interaction between transmembrane helices using ensemble and single-molecule fluorescence resonance energy transfer.
Authors: Yano, Yoshiaki and Kondo, Kotaro and Kitani, Ryota and Yamamoto, Arisa and Matsuzaki, Katsumi
Journal: Biochemistry (2015): 1371-9