Cy3B azide
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 684.86 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.048 |
Correction Factor (280 nm) | 0.069 |
Extinction coefficient (cm -1 M -1) | 1200001 |
Excitation (nm) | 560 |
Emission (nm) | 571 |
Quantum yield | 0.581 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Direct upgrades
iFluor® 555 azide |
Alternative formats
Cy3B maleimide |
Cy3B acid |
Cy3B alkyne |
Related products
Overview | ![]() ![]() |
Upgrade: iFluor® 555 azide
See also: Bioconjugation, Dye to Peptide Conjugation, Chemical Reagents, Classic Dyes, Cyanines, Peptide and Oligonucleotide Labeling
Molecular weight 684.86 | Correction Factor (260 nm) 0.048 | Correction Factor (280 nm) 0.069 | Extinction coefficient (cm -1 M -1) 1200001 | Excitation (nm) 560 | Emission (nm) 571 | Quantum yield 0.581 |
Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. However, Cy3 suffers low fluorescence intensity. It is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3B azide can be used for conjugations with alkyne-containing biological molecules. Cy3® is the trademark of GE Healthcare.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3B azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 146.015 µL | 730.076 µL | 1.46 mL | 7.301 mL | 14.602 mL |
5 mM | 29.203 µL | 146.015 µL | 292.03 µL | 1.46 mL | 2.92 mL |
10 mM | 14.602 µL | 73.008 µL | 146.015 µL | 730.076 µL | 1.46 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Correction Factor (260 nm) | 0.048 |
Correction Factor (280 nm) | 0.069 |
Extinction coefficient (cm -1 M -1) | 1200001 |
Excitation (nm) | 560 |
Emission (nm) | 571 |
Quantum yield | 0.581 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
Cy3B DBCO | 560 | 571 | 1200001 | 0.069 |
Cy3B TCO | 560 | 571 | 1200001 | 0.069 |
Cy3B Tetrazine | 560 | 571 | 1200001 | 0.069 |
AMCA Azide | 346 | 434 | 19000 | 0.153 |
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide* | 490 | 520 | 71000 | 0.11 |
ICG azide | 789 | 814 | - | 0.076 |
XFD647 Azide | 650 | 671 | 270000 | 0.03 |
References
View all 37 references: Citation Explorer
Fluorescence Anisotropy-Based Assay for Characterization of Ligand Binding Dynamics to GPCRs: The Case of Cy3B-Labeled Ligands Binding to MC4 Receptors in Budded Baculoviruses.
Authors: Veiksina, Santa and Tahk, Maris-Johanna and Laasfeld, Tõnis and Link, Reet and Kopanchuk, Sergei and Rinken, Ago
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 119-136
Authors: Veiksina, Santa and Tahk, Maris-Johanna and Laasfeld, Tõnis and Link, Reet and Kopanchuk, Sergei and Rinken, Ago
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 119-136
Quantitative analysis of fluorescent ligand binding to dopamine D3 receptors using live-cell microscopy.
Authors: Allikalt, Anni and Laasfeld, Tõnis and Ilisson, Mihkel and Kopanchuk, Sergei and Rinken, Ago
Journal: The FEBS journal (2021): 1514-1532
Authors: Allikalt, Anni and Laasfeld, Tõnis and Ilisson, Mihkel and Kopanchuk, Sergei and Rinken, Ago
Journal: The FEBS journal (2021): 1514-1532
Fluorescent ligands for dopamine D2/D3 receptors.
Authors: Allikalt, Anni and Purkayastha, Nirupam and Flad, Khajidmaa and Schmidt, Maximilian F and Tabor, Alina and Gmeiner, Peter and Hübner, Harald and Weikert, Dorothee
Journal: Scientific reports (2020): 21842
Authors: Allikalt, Anni and Purkayastha, Nirupam and Flad, Khajidmaa and Schmidt, Maximilian F and Tabor, Alina and Gmeiner, Peter and Hübner, Harald and Weikert, Dorothee
Journal: Scientific reports (2020): 21842
Unifying Mechanism for Thiol-Induced Photoswitching and Photostability of Cyanine Dyes.
Authors: Gidi, Yasser and Payne, Liam and Glembockyte, Viktorija and Michie, Megan S and Schnermann, Martin J and Cosa, Gonzalo
Journal: Journal of the American Chemical Society (2020): 12681-12689
Authors: Gidi, Yasser and Payne, Liam and Glembockyte, Viktorija and Michie, Megan S and Schnermann, Martin J and Cosa, Gonzalo
Journal: Journal of the American Chemical Society (2020): 12681-12689
Fluorescence lifetime imaging with a single-photon SPAD array using long overlapping gates: an experimental and theoretical study.
Authors: Ardelean, Andrei and Ulku, Arin Can and Michalet, Xavier and Charbon, Edoardo and Bruschini, Claudio
Journal: Proceedings of SPIE--the International Society for Optical Engineering (2019)
Authors: Ardelean, Andrei and Ulku, Arin Can and Michalet, Xavier and Charbon, Edoardo and Bruschini, Claudio
Journal: Proceedings of SPIE--the International Society for Optical Engineering (2019)
Redox-Based Photostabilizing Agents in Fluorescence Imaging: The Hidden Role of Intersystem Crossing in Geminate Radical Ion Pairs.
Authors: Glembockyte, Viktorija and Cosa, Gonzalo
Journal: Journal of the American Chemical Society (2017): 13227-13233
Authors: Glembockyte, Viktorija and Cosa, Gonzalo
Journal: Journal of the American Chemical Society (2017): 13227-13233
Characterization of ligand binding to melanocortin 4 receptors using fluorescent peptides with improved kinetic properties.
Authors: Link, Reet and Veiksina, Santa and Rinken, Ago and Kopanchuk, Sergei
Journal: European journal of pharmacology (2017): 58-66
Authors: Link, Reet and Veiksina, Santa and Rinken, Ago and Kopanchuk, Sergei
Journal: European journal of pharmacology (2017): 58-66
Predicting signatures of anisotropic resonance energy transfer in dye-functionalized nanoparticles.
Authors: Gil, Gabriel and Corni, Stefano and Delgado, Alain and Bertoni, Andrea and Goldoni, Guido
Journal: RSC advances (2016): 104648-104656
Authors: Gil, Gabriel and Corni, Stefano and Delgado, Alain and Bertoni, Andrea and Goldoni, Guido
Journal: RSC advances (2016): 104648-104656
Homogeneous fluorescence anisotropy-based assay for characterization of ligand binding dynamics to GPCRs in budded baculoviruses: the case of Cy3B-NDP-α-MSH binding to MC4 receptors.
Authors: Veiksina, Santa and Kopanchuk, Sergei and Mazina, Olga and Link, Reet and Lille, Anne and Rinken, Ago
Journal: Methods in molecular biology (Clifton, N.J.) (2015): 37-50
Authors: Veiksina, Santa and Kopanchuk, Sergei and Mazina, Olga and Link, Reet and Lille, Anne and Rinken, Ago
Journal: Methods in molecular biology (Clifton, N.J.) (2015): 37-50
Cholesterol-induced lipophobic interaction between transmembrane helices using ensemble and single-molecule fluorescence resonance energy transfer.
Authors: Yano, Yoshiaki and Kondo, Kotaro and Kitani, Ryota and Yamamoto, Arisa and Matsuzaki, Katsumi
Journal: Biochemistry (2015): 1371-9
Authors: Yano, Yoshiaki and Kondo, Kotaro and Kitani, Ryota and Yamamoto, Arisa and Matsuzaki, Katsumi
Journal: Biochemistry (2015): 1371-9