Cy3B PEG12 NHS ester

• Amine-reactive NHS ester for efficient conjugation to primary amines on proteins, peptides, and other biomolecules
• Cy3B fluorophore offers superior brightness and photostability compared to traditional Cy3 dyes
• PEG12 spacer enhances water solubility and reduces nonspecific binding and steric hindrance
• Excitation/Emission maxima: ~559 nm / 570 nm, compatible with Cy3 filter sets
• Ideal for high-sensitivity applications such as fluorescence microscopy, flow cytometry, FISH, and single-molecule imaging

Cy3B PEG12 NHS ester is a bright, amine-reactive fluorescent labeling reagent that combines the superior photostability of the Cy3B fluorophore with the enhanced solubility and reduced steric hindrance of a PEG12 spacer. This compound features an N-hydroxysuccinimide (NHS) ester functional group, which reacts efficiently with primary amines on proteins, peptides, and other biomolecules under mild conditions. The inclusion of a PEG12 linker improves hydrophilicity and minimizes nonspecific interactions, making it especially suitable for applications that require high labeling specificity and minimal background signal. Cy3B is known for its high quantum yield and resistance to photobleaching, offering significant advantages over traditional Cy3 dyes in demanding applications such as fluorescence microscopy, flow cytometry, and single-molecule detection. With excitation and emission maxima around 560 nm and 571 nm, respectively, Cy3B PEG12 NHS ester is compatible with standard Cy3 filter sets and serves as a reliable choice for the development of robust, high-performance fluorescent probes.